三坐标测量机在磁基准中的应用

本文对三坐标测量机（CMM）的用途、基本工作原理、使用工具和方法论以及六级聚焦进行探讨,指出其优势和广阔的应用前景,同时也指出了需要注意的几个方面。     

Herceptin functionalized microfluidic polydimethylsiloxane devices for the capture of human epidermal growth factor receptor 2 positive circulating breast cancer cells

Building on recent breakthroughs in the field of microfluidic-based capture of rare cancer cells circulating in the blood, the present article reports on the use of Herceptin functionalized PDMS devices designed to efficiently capture from blood cancer cells, overexpressing the tyrosine kinase human epidermal growth factor receptor (HER2). The identification of patients overexpressing HER2 is critical as it typically associates with an aggressive disease course in breast cancer and poor prognosis. Importantly, HER2 positive patients have been found to significantly benefit from Herceptin (Trastuzumab), a humanized monoclonal antibody (MAb) against HER2. Disposable PDMS devices prepared using standard soft lithography were functionalized by the plasma polymerization of an epoxy-containing monomer. The epoxy-rich thin film (AGEpp) thus created could be conjugated with Herceptin either directly or through a polyethylene glycol interlayer. The properties and reactivity toward the monoclonal antibody conjugation of these coatings were determined using x-ray photoelectron spectroscopy; direct conjugation provided a good compromise in reactivity and resistance to biologically nonspecific fouling and was selected. Using the breast cancer cell line SK-BR-3 as a model for cells overexpressing HER2, the immunocapture efficacy of the Herceptin functionalized PDMS was demonstrated in model studies. Validation studies confirmed the ability of the device to efficiently capture (～80% capture yield) HER2 positive cells from full blood.     

我们都曾是哭鼻子的小屁孩儿

那时花开,流水芳年。 青春很锋利,很容易就划开记忆的容颜,岁月如梭,稍纵即逝,我们还没来得及回忆青涩和苦涩,感受阵痛和欣喜,时间就呼啸而过了。     

虎年说虎

——现役脑残之王:马布里作为当年96黄金一代的球员之一,马布里如今的生活简直可以用穷困潦倒来形容,自从当年他自称天下第一控卫起,马布里的生涯评定便每况愈下。自此,马布里就开始了与全世界对抗的戏码。最初在森林狼,他就因不满加内特拿到亿元高薪而愤然离开,拆散了一对本该是黄金搭档的组合。在转投纽约后,尽管身为纽约之子,但尼克斯宁可掏出两千万也要请这位大爷走人。自从被尼克斯队抛弃之     

终极娱乐——给魔术师和心灵魔术师的导演笔记（节选）

第二章神秘娱乐的层次关系 从我们的这边看出去,我们带给观众的表演是魔术效果（effect）。而从观众的角度来看呢,我们的演出可分成三种不同的感受：     

Extra-curricular participation in sports and socio-demographic factors in Spanish adolescents: the AVENA study

The aims of this study were to identify differences between the sexes in extra-curricular participation in sports and to determine its association with body fat and socio-demographic factors in Spanish adolescents. A total of 2165 adolescents (1124 males and 1041 females) aged 13.0-18.5 years from the AVENA Study participated. Participants filled in an ad hoc questionnaire for extra-curricular participation in sports, which was the dependent variable. Independent variables were: age, percent body fat, and father's and mother's educational level and occupation. Chi-square tests and logistic regression were applied. Bivariate analysis showed for male adolescents that age and father's occupation were related to extra-curricular participation in sports. In addition, body fat and mother's education and occupation (all P?相似文献   

创造奇迹的人

福泽林盖从不相信＂奇迹＂。但在他坚决否认这个世界有奇迹的时候,他发现自己就能创造奇迹。那是一个停电的夜晚,他当时正与他的朋友托德·比米什在一家酒吧喝酒。