一种基于WEB的在线考试系统身份认证方案的设计与实现

针对基于WEB的在线考试系统如何解决登录考试系统的身份认证以及页面之间的认证,给出了一种合理的解决方案。该方案成功、有效地在本校计算机病例模拟考试系统中运用。     

Mallat和小波算法在车牌定位系统中的研究

为了解决含噪车牌图像的定位问题,文章对小波分析在图像的降噪和边缘检测的原理及算法方面进行了深入的研究,在此基础上提出了一种基于Mallat和小波分析的边缘检测和图像分割方法。根据该方法,开发了基于VC＋＋6.0的定位系统,实验结果表明,定位效果好,分割精度高,可以达到98.5%的准确率。     

环境工程本科专业教育与职业拓展有机结合的探讨

环境专业教育是中国环境工作中最重要的一个环节,其主要任务是有计划地培养环保科技人才和管理人才。本文对当前环境工程本科专业教育、毕业生就业状况和未来对环保人才的需求和要求进行了分析,初步探讨了专业教育与从业要求及职业拓展的适应性调整,为环境工程专业本科教学改革与发展提供新的思路。     

将民办高校大学生思想政治教育生活化

当代大学生信息量大、思想活跃．在纷繁复杂的社会生活中大学生的思想政治教育工作越加重要。     

简论病毒式网络视频广告的发展

病毒式网络视频广告是一种新兴的网络广告形式,它以相对低廉的广告制作费用在互联网上将产品或服务的信息以创意化的手段展现给受众,并刺激他们主动、迅速、有效地将广告内容传播给更多的人,从而产生极大的影响力。病毒式网络视频广告从早期的＂推动式＂发展到现在的＂交互式＂,＂毒性＂变得更强,＂参与性＂变得更高。     

浅谈形成性评价在体职院英语教学中的运用

我们在探究如何提高体职院学生的英语教学效果的时候,不仅要注意教材、教法、工具手段,也应该重视评价方式。而形成性评价对改善英语课的教学活动效果起着重要的作用。本文以浙江体育职业技术学院的竞技体育专业的学生为范例,分析了如何在体职院的英语课堂上运用形成性评价的方式来促进学生参与各种课堂交际活动和小组合作活动的积极性。     

浅析苏州红木雕在中国木雕界的杰出地位

本文基于传统手工艺木雕的研究,展示了丰富多彩的木雕历史、工艺介绍。今天远离了手工艺的我们,在审视和研究手工艺时,对它的评价是不高的,可喜的是苏州木雕并没有被人们所遗忘,并没有被历史的尘沙所掩埋。越来越多的人开始关注它,因为这是历史留下来的瑰宝,是值得后代人去继承和探讨的。     

幼师生心理危机预警模型研究

幼师生心理危机是影响学校稳定和发展的重要因素。针对预警在幼师生心理危机管理中的重要性,结合心理危机产生的原因,提出了幼师生心理危机预警指标体系。考虑到指标信息的模糊性,引入多层次模糊综合评判方法,建立了幼师生心理危机预警模型,以期在实践中得到应用。     

Fabrication of a gel particle array in a microfluidic device for bioassays of protein and glucose in human urine samples

This paper describes a simple method for fabricating a series of poly(ethylene glycol) diacrylate (PEG-DA) hydrogel microstructures inside microfluidic channels as probe for proteins and glucose. In order to demonstrate the feasibility of this newly developed system, bovine serum albumin (BSA) was chosen as a model protein. PEG microcolumns were used for the parallel detection of multiple components. Using tetrabromophenol blue (TBPB) and the horseradish peroxidase/glucose oxidase reaction system, bovine serum albumin (BSA) and glucose in human urine were detected by color changes. The color changes for BSA within a concentration range of 1-150 μM, and glucose within a range of 50 mM-2 M could be directly distinguished by eyes or precisely identified by optical microscope. To show the practicability of the gel particle array, protein and glucose concentrations of real human urine samples were determined, resulting in a good correlation with hospital analysis. Notably, only a 5 μL sample was needed for a parallel measurement of both analytes. Conveniently, no special readout equipment or power source was required during the diagnosis process, which is promising for an application in rapid point-of-care diagnosis.     

Label-free microfluidic characterization of temperature-dependent biomolecular interactions

We present a microfluidic approach to characterizing temperature-dependent biomolecular interactions. Solvated L-arginine vasopressin (AVP) and its immobilized RNA aptamer (spiegelmer) were allowed to achieve equilibrium binding in a microchip at a series of selected temperatures. Unbound AVP were collected and analyzed with matrix-assisted laser desorption∕ionization mass spectrometry (MALDI-MS), yielding melting curves that reveal highly temperature-dependent zones in which affinity binding (36–45 °C) or dissociation (25–33 °C and 50–65 °C) occurs. Additionally, temperature-dependent binding isotherms were constructed; from these, thermodynamic quantities involved in binding were extracted. The results illustrated a strong change in heat capacity of interaction for this system, suggesting a considerable thermodynamic influence controlling vasopressin-spiegelmer interaction.