Situated trajectories of learning in vocational training interactions

This paper investigates smaller-scale transitions that are part of the longer-term processes of subjective transformation and adaptation to new professional competencies for learners in the field of vocational education. On the conceptual level, it proposes to view transitions as intermediate states in situated trajectories of learning. The notion of trajectory aims to capture that (a) learning occurs through situated and highly contextualized micro activities and (b) that these activities occur within historical sequences of events, which come to form over time dynamic trajectories. The ingredients constitutive of a trajectory of learning are first defined. The notions presented are next applied to the empirical analysis of one sequence of learning, in which we show the transitions undergone by one object of knowledge as it is being taught to different apprentices in a workshop. On the theoretical plane, the paper thus proposes to view transitions as microdynamics of change. On the methodological plane, it contributes to identifying possible empirical sources and methodological frames to study micro-transitional phenomena.     

Screening the active constituents of Chinese medicinal herbs as potent inhibitors of Cdc25 tyrosine phosphatase, an activator of the mitosis-inducing p34cdc2 kinase

Objective: To screen and evaluate the active constituents of Chinese medicinal herbs as potent inhibitors of Cdc25 phosphatase. Methods: The affinity chromatography purified glutashione-S-transferase/Cdc25A phosphatase fusion protein and Cdc2/cyclin B from the extracts of starfish M phase oocytes are used as the cell cycle-specific targets for screening the antimitotic constituents. We tested 9 extracts isolated from the Chinese medicinal herbs and vegetables including the agents currently used in cancer treatment by measuring the inhibition of Cdc25A phosphatase and Cdc2 kinase activity. The antitumor activity of the extracts was also evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and flow cytometry. Results: Cdc25A inhibitory activity and antitumor activity are detected in the extracts isolated from three Chinese medicinal herbs Agrimona pilosa; Herba solani lyrati; Galla chinesis. Conclusion: We found three extracts isolated from Chinese medicinal herbs have potential inhibitory activity of Cdc25 phosphatase using a highly specific mechanism-based screen assay for antimitotic drug discovery.     

Fingertip force and electromyography of finger flexor muscles during a prolonged intermittent exercise in elite climbers and sedentary individuals

Abstract

The aim of this study was to characterize forearm muscle fatigue identified by the decrease in electromyogram median frequency and/or fingertip force during intermittent exercise. Nine elite climbers (international competitive level, USA 5.14a on sight) and ten non-climbers were instructed to maintain a fingertip force of 80% of their maximal voluntary contraction force on a dynamometer mimicking a rock climbing grip during a 5 s effort/5 s rest cycle for 36 repetitions (i.e. 6 min of exercise). Elite climbers lasted twice as long as non-climbers (climbers: 3 min; non-climbers: 1 min 30 s) before the force could no longer be maintained (i.e. the failure point). After this moment, fingertip force decreased and stabilized until the end of the exercise around 50% maximum voluntary contraction force in non-climbers and 63% in elite climbers. Electromyogram median frequency showed a greater decrease in non-climbers than in elite climbers before the failure point. No change in median frequency was observed after the failure point in elite climbers or in non-climbers. These results confirm that elite climbers are better adapted than non-climbers for performing the intermittent fingertip effort before the failure point. After this point, the better fingertip force of elite climbers suggests different forearm muscle properties, while the electromyography results do not provide any indication about the fatigue process.     

A comparison of methods to determine maximal accumulated oxygen deficit in running

Abstract

The aim of this study was to compare maximal accumulated oxygen deficit (hereafter O₂ deficit) estimated from the methods of Whipp et al. (1986 Whipp, B. J., Ward, S. A. and Wasserman, K. 1986. Respiratory markers of the anaerobic threshold. Advances in Cardiology, 35: 47–64. [Crossref], [PubMed] , [Google Scholar]), Medbo et al. (1988 Medbo, J. I., Mohn, A. C., Tabata, I., Bahr, R., Vaage, O. and Sejersted, O. M. 1988. Anaerobic capacity determined by maximal accumulated O₂ deficit. Journal of Applied Physiology, 64: 50–60. [PubMed], [Web of Science ®] , [Google Scholar]), and Hill et al. (1998 Hill, D. W., Ferguson, C. S. and Ehler, K. L. 1998. An alternative method to determine maximal accumulated O₂ deficit in runners. European Journal of Applied Physiology, 79: 114–117. [Crossref], [Web of Science ®] , [Google Scholar]) to determine whether they agree sufficiently to be used interchangeably. Nineteen moderately to highly trained endurance runners first performed an incremental test to exhaustion for the determination of maximal oxygen uptake ([Vdot]O_2max) and peak treadmill speed, followed by six randomly ordered constant-speed tests at 95, 100, 105, 110, 110, and 120% of peak treadmill speed. All tests were separated by at least 72 h and were performed within 4 weeks. The method of Whipp produced an O₂ deficit estimate that was lower than that derived from the method of Hill or Medbo (bias ± 95% limits of agreement: ?29.6 ± 36.6 and ?26.1 ± 32.8 ml · kg^?1, respectively; P < 0.001). The O₂ deficit did not differ between the methods of Hill and Medbo (bias ± 95% limits of agreement: 3.5 ± 41.6 ml · kg^?1; n.s.). However, poor correlations (0.21 < r < 0.33; n.s.) together with wide limits of agreement between O₂ deficit estimates (70 – 80% of the mean response) clearly question using these methods interchangeably.     

Impact of the choice of threshold on physical activity patterns in free living conditions among adolescents measured using a uniaxial accelerometer: The HELENA study

Abstract

The aim of this study was to assess the impact of the choice of threshold on physical activity patterns measured in adolescents under free living conditions (FLC) using a uniaxial accelerometer. The study comprised 2043 adolescents (12.5–17.5 years) participating in the HELENA Study. Participants wore a uniaxial accelerometer for 7 days. The PA patterns were assessed using thresholds determined from six different studies. For each of the thresholds used, the number of adolescents fulfilling the recommendation of 60 min of moderate to vigorous PA (MVPA) per day was also calculated. A significant difference was found between thresholds regardless of the activity level: differences of 38%, 207%, 136%, and 2780% for sedentary, light, moderate, and vigorous intensity PA, respectively (P < 0.001). Time of MVPA varied between methods from 25.3 to 55.2 min · day^–1. The number of adolescents fulfilling the recommendation varied from 5.9% to 37% according to the thresholds used. The kappa coefficient for concordance in the assessment of the number of adolescents achieving the PA recommendations was generally low. The definition of the threshold for PA intensity may considerably affect the PA patterns in FLC when assessed using a uniaxial accelerometer and the number of participants fulfilling the recommendations.     

The effects of task constraints on the organization of interception movements

In the light of the intensity coupling effects reported in the literature, subjects' capacities for independently controlling the absolute velocity of their movement at the point of interception was evaluated by asking them to learn to propel orthogonally approaching balls, varying in their speed of approach, into target boxes placed at various distances from the interception point. As required for successful accomplishment of the task, movement velocity was found to be adapted to the distance to be covered, with the near target giving rise to lower velocities and the far target giving rise to higher velocities even when the conditions were presented in random order. Nevertheless, even though target distance accounted for a substantial proportion of the total variance, a small but significant effect of ball approach speed on movement velocity was found, suggesting that intensity coupling is a persistent but modulable phenomenon in interception tasks.     

The role of the entry-and-stretch phase at the different paces of race in front crawl swimming

The aim of this study was to determine the role played by the entry-and-stretch phase in the coordination of swimming, at the different paces of race. Three national level swimmers (two men and one woman) were recorded, in lateral and bottom views, in three swimming paces: sprint (50 m and 100 m), middle-distance (200 m and 400 m) and long-distance (800 m and 1500 m). Anatomical landmark positions were obtained by manual digitalisation of the videos. Computational fluid dynamics and experimental studies (with a strain gauge balance and particle image velocimetry method) were used to measure and to calculate the external forces applied to the hand and to the forearm and to visualise the flow around the profile. Entry-and-stretch is the phase which varies the most according to the swimming pace. This phase can be decomposed into two sub-phases: one, the extension forward coordinated with the insweep of the opposite arm, and another one, the rotation downward coordinated with the upsweep. Results show that, at the three paces, this phase is not propulsive and could contribute essentially to maintain the horizontal balance of the body.     

Physical activity awareness of European adolescents: The HELENA study

The aim of this study was to assess physical activity (PA) awareness of adolescents and to identify anthropometric and psychosocial factors that can lead to under or overestimation of PA. This study included 2044 adolescents. Participants wore a uniaxial accelerometer for 7 days to measure PA and completed a self-rated questionnaire about PA. Adolescents were classified into four PA awareness subgroups (realistically active, overestimators, underestimators and realistically inactive) according to the self-rated and objective assessment of PA. Characteristics and psychosocial factors of the self-rated physically active groups were compared using bivariate and multivariate mixed logistic regression models. Forty-five percent of adolescents reported their PA levels correctly (34.8% realistically active and 10.1% realistically inactive). Among the 59.4% who were objectively inactive, 82.9% tended to overestimate their PA level. Adolescents who overestimated their PA level were older (P < .05), had more support from mother and a best friend (P < .05) and had higher cardiorespiratory fitness (P < .001) compared with those who were realistically active. A significant number of adolescents believe that they are physically active when they are not. Improving awareness, especially in the high-risk groups identified here, might help to bring about behavioural changes in physically inactive adolescents.     

Greater fatigability in knee-flexors vs. knee-extensors after a standardized fatiguing protocol

The present study aimed to investigate the effects of a standardized fatiguing protocol on central and peripheral fatigue in knee-flexors and knee-extensors. Thirteen healthy men (age: 23?±?3 years; height: 1.78?±?0.09 m; body-mass: 73.6?±?9.2?kg) volunteered for the present study. Maximal voluntary contraction (MVC), Electromyography (EMG) activity, voluntary activation level (VAL) as an index of central fatigue and twitch potentiation as an index of peripheral fatigue were measured before and after the fatiguing protocol. The fatiguing protocol consisted of a 0.6 duty-cycle to exhaustion (6?s isometric contraction, 4?s recovery) at 70% MVC. After the fatiguing protocol, MVC decreased in both (Effect-size (ES)?=?1.14) and knee-extensors (ES?=?1.14), and EMG activity increased in both knee-flexors (ES?=?2.33) and knee-extensors (ES?=?1.54). Decreases in VAL occurred in knee-flexors (ES?=?0.92) but not in knee-extensors (ES?=?0.04). Decreases in potentiation occurred in both knee-flexors (ES?=?0.84) and knee-extensors (ES?=?0.58). The greater central occurrence of fatigue in knee-flexors than in knee-extensors may depend on the different muscle morphology and coupled with a greater tolerance to fatigue in knee-extensors. The present data add further insight to the complicated knee-flexors-to-knee-extensors strength relationship and the mechanisms behind the different occurrence of fatigue.