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Ioannis N. Katis Judith A. Holloway Jens Madsen Saul N. Faust Spiros D. Garbis Peter J. S. Smith David Voegeli Dan L. Bader Robert W. Eason Collin L. Sones 《Biomicrofluidics》2014,8(3)
We report the Laser Induced Forward Transfer (LIFT) of antibodies from a liquid donor film onto paper receivers for application as point-of-care diagnostic sensors. To minimise the loss of functionality of the active biomolecules during transfer, a dynamic release layer was employed to shield the biomaterial from direct exposure to the pulsed laser source. Cellulose paper was chosen as the ideal receiver because of its inherent bio-compatibility, liquid transport properties, wide availability and low cost, all of which make it an efficient and suitable platform for point-of-care diagnostic sensors. Both enzyme-tagged and untagged IgG antibodies were LIFT-printed and their functionality was confirmed via a colorimetric enzyme-linked immunosorbent assay. Localisation of the printed antibodies was exhibited, which can allow the creation of complex 2-d patterns such as QR codes or letters for use in a final working device. Finally, a calibration curve was determined that related the intensity of the colour obtained to the concentration of active antibodies to enable quantitative assessment of the device performance. The motivation for this work was to implement a laser-based procedure for manufacturing low-cost, point-of-care diagnostic devices on paper. 相似文献
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Harriet L. Schwartz Elizabeth L. Holloway 《Mentoring & Tutoring: Partnership in Learning》2013,21(1):115-135
Meaningful academic relationships between adult master’s students and their professors can both deepen students’ learning and serve as a regenerating force for professors. This study employed grounded theory methods to explore the question, “What goes on in relational practice between master’s students and professors?” I interviewed 10 matched pairs of recent alumni and professors who identified as having “a meaningful academic relationship”. Dimensional analysis surfaced two core dimensions: reconstructing and regenerating. In reconstructing, the students’ core dimension reveals the student experience of reconstructing, or understanding more deeply, theory or one’s self. In the case of regenerating, the professor’s core dimension identifies the professors’ experience of “giving back” through their teaching and extending their professional reach by training others. These experiences serve to reinvigorate professors over the course of their careers. In addition, findings in this study resonate with sensitizing concepts including relational cultural theory and relational practice. Finally, the analysis surfaced evidence supporting authentic teaching concepts and connected these concepts to faculty and student learning partnerships. 相似文献
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Susan M. Holloway Patricia A. Gouthro 《Discourse: Studies in the Cultural Politics of Education》2011,32(1):29-41
Drawing upon our experience as university faculty in teaching novice educators, we explore the issue of resistance from students around learning that entails critical reflection. By novice educators, we refer to pre-service teacher candidates and graduate students in Education faculties, particularly graduate students in adult education/lifelong learning programs, who often do not have prior Education degrees or extensive experience as educators. We believe novice educators should be taught to be critically reflective, regardless of whether they teach in schools, government, the not-for-profit sector, or business sector. 相似文献
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Previous work suggests that children largely perceive science as the passive observation and recording of events. In contrast, studies of practicing scientists show a concern with building and testing models. In this study we investigated the role of a design context for developing children's understanding of science as the construction and revision of models. Grade 1–2 children were given the task of building a model that works like a human elbow. Via discussion, model building, evaluation, and revision, children came to understand that not only motion but also constraints on motion were important qualities to include in their models. Moreover, review of classroom activity and analysis of the postmodeling interview suggest that as early as first grade, children's model-evaluation skills may be quite amenable to development: In comparison to a nonmodeling peer group, modelers were largely able to ignore perceptual qualities when asked to judge the functional qualities of models. Further, they showed an understanding of the modeling process in general that was similar to that of children 3–4 years older. © 1997 John Wiley & Sons, Inc. J Res Sci Teach 34 : 125–143, 1997. 相似文献
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Jiří Baláš David Giles Leona Chrastinová Kateřina Kárníková Jan Kodejška Alžběta Hlaváčková 《Journal of sports sciences》2017,35(10):989-994
The aim of this study was to examine the effect of alterations in potential lead fall distance on the hormonal responses of rock climbers. Nine advanced female climbers completed two routes while clipping all (PRO-all) or half (PRO-½) of the fixed points of protection. Venous blood samples were analysed for total catecholamines, noradrenaline (norepinephrine), adrenaline (epinephrine), dopamine, lactate, cortisol and serotonin. Differences between the two conditions pre, immediately post and 15 min post climbing were assessed using a 2 × 3 repeated measures ANOVA. All hormones and blood lactate concentrations increased significantly (P < 0.05) immediately post climb, except for cortisol. Peak cortisol concentrations did not occur until 15 min post ascent. Further, significant interactions between climbing and clipping conditions were found for total catecholamines (890% of basal concentration in PRO-½ vs. 568% in PRO-all), noradrenaline (794% vs. 532%) and dopamine (500% vs. 210%). There were no significant interactions for adrenaline (1920% vs. 1045%), serotonin (150% vs. 127%) or lactate (329% vs. 279%). The study showed a greater catecholamine response with an increase in potential lead fall distance. The most pronounced increases seen in catecholamine concentration were reported for dopamine and noradrenaline. 相似文献
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Jeffrey N. Wherry Nick Paal John B. Jolly Balkozar Adam Carol Holloway Betty Everett Lela Vaught 《Psychology in the schools》1993,30(1):29-36
The discriminant and concurrent validity of the Gordon Diagnostic System (GDS) was investigated in 29 youngsters categorized into “normals” or “ADHDs” based on teacher ratings. The results failed to demonstrate the discriminant validity of any GDS score regardless of the behavior rating used. The Vigilance Correct and Vigilance Omission scores were significantly correlated with ADHD Rating Scale scores completed by teachers. The sample size in the study demands cautious interpretation of these results; however, the authors suggest the continued use of multiple behavior ratings by teachers as the “gold standard” for the classification of youngsters with a suspected Attention-deficit Hyperactivity Disorder. 相似文献