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41.
42.
以EGFP为标记基因,利用重新构建的pAV-EGFP质粒,采用直接注射方法,进行了离体和在体基因转移研究。实验结果显示,经脂质体包裹后,质粒可以有效地进行细胞转移;DNA表达载体肌肉直接注射后,利用共聚焦显微镜可以在注射点周围的肌细胞观察到绿色荧光蛋白;而在对侧肢PCR和共聚焦显微镜的结果均为阴性。实验结果表明,肌肉直接注射DNA可以在肌肉得到表达,将会为治疗肌肉损伤带来新的方法。 相似文献
43.
Transcribing sign communication used simultaneously with spoken English presents investigators with a unique problem: the singular quality of the bimodal communicative interactions cannot be accurately depicted using accepted conventions for recording either spoken or signed language samples. This article proposes guidelines for transcribing such data. The need for guidelines arose during an earlier study of the language development of a hearing child of deaf parents. To meet the immediate needs of that study, rules and conventions from previous studies were combined with newly generated ones, resulting in the guidelines proposed in this article. The guidelines can be applied to data in which intermodal linguistic influence is suspected. 相似文献
44.
Irvin J. Lehmann 《Educational Measurement》1990,9(1):3-10
What are the roots of NCME? What were our initial goals and how well have we attained them? Are we drifting away from our practitioner orientation? What should be our policy on important matters in educational practice and our role in sociopolitical issues in educational measurement? 相似文献
45.
目前使用机器学习方法对稀有的PPII二级结构进行预测工作还比较少见.本文在分析数据集的特征基础之上,采用加强局部信息的改进编码方法,并使用生物信息学中常用的BP神经网络来预测PPII二级结构.通过对不同输入窗口长度与不同隐层节点数的神经网络进行训练和测试,得出在输入窗口长度为5个氨基酸残基和隐结点数为8时PPII螺旋结构能够被很好地预测出来,此时的预测敏感度为82.5%. 相似文献
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V. J. Hinton D. C. De Vivo R. Fee E. Goldstein Y. Stern 《Learning disabilities research & practice》2004,19(3):146-154
Duchenne Muscular Dystrophy (DMD) is a neurogenetic developmental disorder that presents with progressive muscular weakness. It is caused by a mutation in a gene that results in the absence of specific products that normally localize to muscle cells and the central nervous system (CNS). The majority of affected individuals have IQs within the normal range, generally with lower verbal than performance IQ scores. Prior work has demonstrated selective deficits on tests of verbal span and immediate memory. For the current study, 26 boys with DMD (and normal intellectual function) and their unaffected siblings were evaluated. Paired comparisons demonstrated that the children with DMD had significantly poorer academic achievement scores than their siblings, even though their vocabulary levels and home and educational environments were comparable. Children with DMD also had more behavioral concerns, physical disabilities, and poorer verbal memory spans. Linear regression indicated that behavioral concerns, executive function, and physical disability did not contribute substantially to academic performance, whereas performance on verbal span did. DMD presents with a selective developmental aberration in verbal span that has wide‐ranging consequences on learning skills. 相似文献
49.
As a part of efforts to evaluate and monitor the increasing public investment in early childhood education, teachers are being asked to assess children's school readiness. In this study, preschool teachers and kindergarten teachers rated children's skills in three areas (kindergarten readiness, academic skills, and communication skills), and these ratings were compared with direct assessments of the children's skills. Ratings by both groups of teachers tended to be more highly related to basic skills, such as counting and number naming, than to abilities such as solving applied problems and using expressive and receptive vocabulary. Preschool teachers' ratings had a lower association with children's observed skills and abilities than kindergarten teachers' ratings. Ratings of children attending Head Start were systematically inflated, but this relationship was mediated to a significant extent by the teachers' levels of education. More educated teachers rated children in a manner consistent with the children's directly assessed skills. Implications of these findings for informing future efforts to assess school readiness by using teacher ratings are discussed. 相似文献
50.
博物馆与传承和保护传统手工艺 总被引:3,自引:0,他引:3
传统手工艺作为一种重要的化遗产,其有形的物质载体的保护已得到相当重视,而其历史积累的工艺流程、操作技巧、技术经验等无形财产的保护,亟待引起足够的重视。长期以来,博物馆一直是担负科学保护化遗产重任的最有力、最重要的机构,在传承和保护传统手工艺有形的和无形的遗产中,应如何利用自己的设施、人才和经验方面的优势发挥作用,履行应尽的职责? 相似文献