Designing and validating an autoverification system of biochemical test results in Hatay Mustafa Kemal University,clinical laboratory

IntroductionAutoverification (AV) is a postanalytical tool that uses algorithms to validate test results according to specified criteria. The Clinical and Laboratory Standard Institute (CLSI) document for AV of clinical laboratory test result (AUTO-10A) includes recommendations for laboratories needing guidance on implementation of AV algorithms. The aim was to design and validate the AV algorithm for biochemical tests.Materials and methodsCriteria were defined according to AUTO-10A. Three different approaches for algorithm were used as result limit checks, which are reference range, reference range ± total allowable error, and 2nd and 98th percentile values. To validate the algorithm, 720 cases in middleware were tested. For actual cases, 3,188,095 results and 194,520 reports in laboratory information system (LIS) were evaluated using the AV system. Cohen’s kappa (κ) was calculated to determine the degree of agreement between seven independent reviewers and the AV system.ResultsThe AV passing rate was found between 77% and 85%. The highest rates of AV were in alanine transaminase (ALT), direct bilirubin (DBIL), and magnesium (Mg), which all had AV rates exceeding 85%. The most common reason for non-validated results was the result limit check (41%). A total of 328 reports evaluated by reviewers were compared to AV system. The statistical analysis resulted in a κ value between 0.39 and 0.63 (P < 0.001) and an agreement rate between 79% and 88%.ConclusionsOur improved model can help laboratories design, build, and validate AV systems and be used as starting point for different test groups.     

我国高校编辑出版教育刍议

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我国加入WTO后，企业竞争将会更加残酷也更加激烈。企业竞争取胜的关键在于努力塑造和选择合适的竞争优势。而塑造和选择合适的竞争优势，需要企业在认知和把握竞争优势内涵的基础上，分析创建竞争优势的各种途径，并通过取舍和整合方式选择更能发挥自身优势的方法，将自己的资源和能力集中于这些方法，创建强大的竞争优势。     

利用期权理论对债转股比例的定量研究

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基本RS触发器电路结构教学探讨

基本RS触发器是最简单的触发器,是构成其它类型触发器的基本单元.     

面向国家战略需求 实现生命科学与生物技术的跨越发展

生命科学与生物技术直接关系到社会发展、国民经济增长、食物安全,城乡人民健康水平和生活环境质量的改善.本文从国家战略需求和科学发展态势的分析入手,阐述了我院生命科学与生物技术的竞争地位和发展动态,从学科部署、研究平台建设和队伍建设等方面提出了跨越发展的战略举措.     

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有着姣好的气质、锐利的眼神、可塑性极高的外型,童星出身的吉娜·马隆至今已出道20多年,演出超过20部电影,类型横跨恐怖、古装、经典文学以及奇幻电影等,可算是好莱坞新一代女星中实力强劲的演技派。马隆的童年生活可谓是居无定所,她由同性恋母亲及其女友一同抚养长大,在她9岁前就搬过27次家,丰富的生活经历为马隆从事演员这一职业打下了基础。1996年,12岁的马隆就凭借着电影处女作《卡罗莱纳     

新烟碱杀虫剂噻虫嗪免疫学特性研究

通过化学修饰合成了噻虫嗪（thiamethoxam,TMX）人工半抗原,采用碳二亚胺法（EDC）将该抗原与牛血清蛋白和卵清蛋白偶联成功制备了分子结合比合理的免疫抗原（TMX-BSA）和包被抗原（TMX-OVA）.对经TMX-BSA免疫的6周龄Balb/c实验鼠的免疫抗血清进行了间接ELISA和阻断ELISA,初步明确了抗噻虫嗪多克隆抗体（TMX-pAb）的免疫学特性,为噻虫嗪残留免疫学快速检测技术开发和检测产品研制奠定了坚实基础.     

皖江城市带文化创意产业的区位选择优化路径探析

根据产业区位理论,产业布局有其自身的规律性。合理的产业区位布局有利于产业的健康发展。通过考察发现皖江城市带的文化创意产业的区位分布存在着主观随意性、与当地的资源状况结合程度不够等一些不合理因素。对此应采取以下对策:统一规划,避免重复建设和过于分散化经营现象;充分利用区域资源优势和文化特色打造产业集群;政策引导与市场导向有机结合;打破行政区划的限制,按照市场和消费者群体的空间特征安排产业的空间布局;精心打造创意城市。     

Application of sigma metrics for the assessment of quality assurance using the MQ-2000 PT HbA1c analyzer

Introduction

Glycosylated hemoglobin (HbA1c) concentrations measured in clinical chemistry laboratories show large differences between their interlaboratory reported values. Laboratory measurements of quality performance should be based on quantitative data. The sigma metrics model provides an objective method for the assessment of current HbA1c assays and is useful in quality management planning. The aim of our study was to evaluate the analytical performance of the MQ-2000 PT HbA1c analyzer test results in the context of our operating conditions on the sigma scale.

Materials and methods

The coefficient of variation was determined from the calculated mean and standard deviation evaluated from internal quality control (QC) (N = 168 days) (Shanghai Huachen Biological Reagent Co. Ltd, China) data, and records of external quality data (KBUDEK, İstanbul, Turkey) measured in the period from May to November 2013 were used to determine the bias. The resulting data and total allowable error rate (TEA = 10%) from the Clinical Laboratory Improvement Amendments of 1988 (CLIA’88) were used to calculate the sigma level.

Results

The calculated coefficient of variations (CVs) at the two levels, normal (QC1 = 36.6 ± 2.38 mmol/mol) and pathological (QC2 = 84.7 ± 2.68 mmol/mol), were 6.5% and 3.1%, respectively. The average bias between the external QC and MQ-2000 PT during the study period was 4.3%. The calculated average sigma value was 1.19.

Conclusions

The MQ-2000 PT HbA1c is a new analyser in the market; there is need for improvement and the method should be controlled with greater attention to ensure quality.Key words: diabetes mellitus, hemoglobin A1c protein, human, chromatography, high pressure liquid chromatography