Effect of oral ingestion of different forms of silver on tissue content of some essential elements in chicks

Silver is ingested in India in the form of varak, Ayurvedic bhasm and water contained in silver utensils, but nobody knows their effects. We have examined the effect of interaction of silver ingested in the form of varak (leaf), bhasm (ash) and water (colloidal solution) on some essential elements (Na, K, Ca, Mg, Cu, Fe and Zn) in blood, liver, spleen and kidneys of chicks. The results showed significant rise of silver in all the tissues and significant fall in copper in the blood. The greatest rise was obtained in blood silver by silver sol while it had the lowest amount of silver. Iron significantly increased in tissues but somewhat decreased in blood. Zinc content increased in all the tissues by leaf and ash but decreased by sol. No consistent pattern was seen in case of other elements.     

Effect of vitamin E on protein bound carbhohydrate complexes in radiation treated oral squamous cell carcinoma patients

Serum glycoproteins were evaluated in oral squamous cell carcinoma patients treated with radiotherapy and also the effect of vitamin E was studied. Cell surface glycoconjugates are important parameters in the detection of malignancy. Thus, the objective of the present study is to evaluate the efficacy of vitamin E on glycoproteins in oral cavity cancer patients treated with radiotherapy. The study includes 26 age and sex matched normal healthy individuals and 26 patients with squamous cell carcinoma of oral cavity. These patients were divided into two groups, one for radiotherapy alone (at a dosage of 6000 cGy in five fractions per week for a period of six weeks) and the other for radiotherapy plus vitamin E supplementation (at a dosage of 400 IU / day of vitamin E) for the entire period of radiotherapy. Levels of hexose, hexosamine, fucose and sialic acid were increased in oral squamous cell carcinoma patients and a significant decrease was observed in radiation treated patients when compared to control. The levels of glycoconjugates were significantly decreased in radiation treated patients supplemented with vitamin E. This measurement may be useful in assessing disease progression and identifying patients resistant to therapy and a possible role of vitamin E on reduction in glycoconjugate levels of radiation treated oral squamous cell carcinoma patients.     

hs-CRP and oxidative stress in young CAD patients: A pilot study

Premature coronary artery disease (CAD) is common in India. We, therefore, studied oxidative stress, dyslipidemia, and high sensitivity-C reactive protein (hs-CRP) levels in young CAD patients. Present study consisted of male CAD patients below 40 years and age and sex matched healthy controls (n=30 each). Fasting blood samples were analyzed for serum lipid profile, malondialdehyde, antioxidant enzymes and hs-CRP levels. Dyslipidemia was observed in 90% of the young CAD patients, of which 72.2% showed increased serum triglycerides and decreased HDL-cholesterol. LDL-cholesterol levels were high in 77.8%. Serum malondialdehyde and hs-CRP levels were increased significantly (p<0.0001) as compared to controls. hs-CRP levels were in high risk range in all the young patients. However, glutathione peroxidase activity was reduced significantly (p<0.05). Our data suggests that elevated hs-CRP levels along with dyslipidemia and oxidative stress adds to the predictive value of premature CAD in young Indians.     

Preliminary studies on the detection ofMycobacterium avium-intracellulare complex using DNA probe from a clinical isolate

Genomic DNA from a clinical isolate ofMycobacterium avium-intracellulare complex was purified and cloned in PBR 322 at the tetracycline resistance site using Bam HI restriction enzyme. A 16 kb cloned fragment was purified, radiolabeled and used as a probe. Genomic DNA isolated from nineteen MAC strains, threeM. tuberculosis strains and oneM. kansasii strain were digested with Eco RI restriction enzyme, Southern blotted and hybridized with the 16 kb cloned and labeled fragment. Twelve MAC strains showed positive hybridization although five strains gave faint signals. Positive hybridization was noted in two out of the threeM. tuberculosis strains, possibly due to shared DNA homology. No signal was received from the singleM. kansasii strain used in this study.     

Carbohydrate gel ingestion during running in the heat on markers of gastrointestinal distress

The purpose of this study is to measure the effects of carbohydrate ingestion during exercise in the heat by measuring markers of gastrointestinal damage and inflammation. Methods: Active subjects (n?=?7) completed two 60-min running trials in a heated environment (70% VO₂max, 30°C). At minute 20 of exercise, subjects consumed a carbohydrate gel (Cho) (27?g), or a non-carbohydrate placebo (nCho). Plasma endotoxin, I-FABP, TNF-α, IL-6, IL-1β, IL-10, and MCP-1 were measured pre-exercise, 20-min post-exercise, and again 2-h, and 4-h post-exercise. Results: Endotoxin increased 20-min post-exercise compared to pre in the Cho trial only (p?=?.03). I-FABP levels increased 20-min post-exercise in the Cho trial only compared to pre-exercise (p?=?.003). I-FABP levels were also increased in Cho trial 20-min post-exercise when compared to same time point in the nCho trial (p?=?.032). TNF-α increased 20-min post-exercise in the Cho trial only compared to pre (p?=?.03). Plasma IL-6 concentration increased 20-min post-exercise when compared to pre in both the Cho (p?=?.002) and nCho (p?=?.009), but remained elevated at the 2-h time point in the nCho trial (p?=?.03). I-FABP and several plasma cytokines (TNF-α, MCP-1, Il-6) returned to baseline sooner in the Cho trial. Conclusions: Ingestion of carbohydrate gel during exercise in the heat enhances markers of gastrointestinal wall damage.     

Development and validation of Mobile Learning Acceptance Measure

The growth of Smartphone usage, increased acceptance of electronic learning (E-learning), the availability of high reliability mobile networks and need for flexibility in learning have resulted in the growth of mobile learning (M-learning). This has led to a tremendous interest in the acceptance behaviors related to M-learning users among the information systems researchers. Despite a large amount of significant research in the field of M-learning, the measurement of user acceptance of M-learning has not received attention. This research study intends to develop and validate a survey that measures users’ acceptance of M-learning. A total sample of 806 university and higher college students from different institutes in Oman participated in this study. This research study was conducted in two stages. The first stage using a sample size of 388 students initiated a generic questionnaire, and examined factorial validity and reliability. The second stage that was conducted using different sample of 418 students employed confirmatory factor analysis to establish factorial validity and measurement invariance. A correlated six-factor model (Flexibility, Suitability, Enjoyment, Efficiency, Economic and Social) was fit using maximum likelihood estimation. The internal consistency and item reliability of Mobile Learning Acceptance Measure was found to be at acceptable level for both samples.     

Exploring evidence of reflective thinking in student artifacts of blogging-mapping tool: a design-based research approach

Reflective learning can assist a learner’s purposeful and conscious manipulation of ideas toward meaningful learning and knowledge integration. Blogs have been used to support reflection, but Blogs usually do not provide overt mechanisms for students to make connections between different parts of an experience, which is integral to reflective activity. A blogging-mapping tool was designed to support the reflective learning process. The tool allowed students to write blog posts, attach up to five keywords to each post, and link the keywords on a concept map. This study aimed to seek patterns of participants’ use of the tool, including evidence of reflective learning, identified as integration between concept maps and blogs. Nine graduate students participated in the study for a semester. Data analysis included examining concept maps for knowledge integration over time. Participants’ various mapping activities implied different levels of thinking. Some participants’ final concept maps showed signs that they tried to integrate knowledge around the content area. Yet, the inconsistent and inconclusive patterns of participants’ concept mapping activities signaled that more strategies were needed to sustain their commitment.