Astudy on mycobacterial proteins in the serodiagnosis of tuberculosis using ELISA and western blot

Protein fraction termed P₂ was obtained from sonicates of Mycobacterium smegmatis, subjected to ammonium sulphate precipitation. P₂ fraction was further fractionated into 4 fractions (PeakI-IV) by DEAE cellulose ion-exchange chromatography. ELISA was performed on the sera of 104 tuberculous cases and 62 controls, using P₂ fractions Peak I as test antigens. Though the mean ELISA values in tuberculous cases were higher than the controls, no statistically significant difference was found between the two. Antigens P₂, Peak I and IV were tested on Western blots with pooled sera from tuberculous cases as well as controls. P₂ fraction, Peak-I and IV were separated on PAGE-SDS, electroblotted onto nitrocellulose Sheets and the blots were subjected to ELISA. Peak IV appeared as a single band (M.W. 55,000). P₂ fraction exhibited some discriminatory bands on development of blots.     

‘The more things change…?’: the Thatcher years and educational reform in Scotland

The period of the Thatcher Government continues to have special significance for politics and governance in Scotland. In the 11 years of the Thatcher Government, landmark legislation and reforms affected key areas of the Scottish society and economy. Education featured prominently in the Thatcherite agenda in Scotland. In Scotland, the education system’s association with national identity had particular implications for educational policy making and implementation in Scotland under the Thatcher Government. The distinctiveness of the Scottish education system presented particular problems for a government intent on challenging the social democratic consensus. Opposition to the Thatcher Government, especially its perceived attack on the social democratic underpinnings of the welfare state which included the state schooling system, re‐ignited the home rule campaign in Scotland in the late 1980s. The article examines both key legislation, namely the Education (Scotland) Act 1981, the School Board (Scotland) Act 1988 and the Self‐Governing Schools etc. (Scotland) Act 1989, and also key non‐legislative reforms to curriculum and assessment under the Thatcher Government in the area of public (state) schooling. The article argues that these reforms continue to influence the educational policy debates in Scotland today.     

‘The constant rejections hurt’: Skills and personal attributes needed to successfully complete a thesis by publication

The thesis by publication (TBP) is an increasingly popular approach to doctoral education that sees candidates publishing their research during their candidature, and including these outputs in their final thesis submission. Responsive to the realities of modern academia, a TBP provides doctoral candidates with opportunities to engage in the scholarly publication process, and to build their research portfolio. As the TBP gains further traction in many fields and countries, there is a need to better understand the skills and attributes needed to complete a TBP successfully. This paper draws on the views of 246 recent doctoral graduates of Australian universities to identify the skills and attributes they felt supported their success. Their responses have implications for the development and refinement of institutional policies and programs that are sensitive to the needs and experiences of doctoral candidates seeking to publish during candidature and create a TBP. As editors, peer‐reviewers, and publishers see a growing influx of papers sole‐ or co‐authored by doctoral candidates in response to the trend toward TBP, they may find themselves involved in supporting students to develop the skills and attributes needed to successfully publish during candidature.     

Graduate Student Space and Service Needs: A Recommendation for a Cross-campus Solution

Focus group methodology was used to investigate graduate students' cross-campus technology, space and service needs. Although the library provides valued services and spaces, graduate students need enhanced and more equitable support for their roles as teachers and scholars. Librarians can provide leadership and act as advocates for graduate student needs in partnership with other campus stakeholders.     

Developing and using a rubric for evaluating evidence-based medicine point-of-care tools

Objective:

The research sought to establish a rubric for evaluating evidence-based medicine (EBM) point-of-care tools in a health sciences library.

Methods:

The authors searched the literature for EBM tool evaluations and found that most previous reviews were designed to evaluate the ability of an EBM tool to answer a clinical question. The researchers'' goal was to develop and complete rubrics for assessing these tools based on criteria for a general evaluation of tools (reviewing content, search options, quality control, and grading) and criteria for an evaluation of clinical summaries (searching tools for treatments of common diagnoses and evaluating summaries for quality control).

Results:

Differences between EBM tools'' options, content coverage, and usability were minimal. However, the products'' methods for locating and grading evidence varied widely in transparency and process.

Conclusions:

As EBM tools are constantly updating and evolving, evaluation of these tools needs to be conducted frequently. Standards for evaluating EBM tools need to be established, with one method being the use of objective rubrics. In addition, EBM tools need to provide more information about authorship, reviewers, methods for evidence collection, and grading system employed.

Highlights

• Eleven of the fourteen previous evidence-based medicine (EBM) tool evaluations were based on clinicians evaluating tools based on their perception of the products'' ability to answer a clinical question.
• EBM tools'' evidence summaries are not updated as often as products claim.
• Although many EBM tools claim to be evidence based, only 74% of the 70 evaluated treatment summaries included graded evidence.

Implications

• To offer the best tools for users, medical libraries should evaluate EBM resources regularly, including the quality of the evidence provided.
• Medical librarians have a role to play in evaluating the quality of EBM products and can develop assessment tools to aid in this evaluation.

     

How can Booktok on TikTok inform readers' advisory services for young people?

Libraries provide readers' advisory services to young people to foster reading for pleasure. The Booktok community on TikTok is a resource that can be drawn upon to support promotion of books with this demographic. A hybrid content analysis of 116 TikTok videos explores presence of hashtags, authors and books, and recurring themes promoted in Booktok videos. Popular hashtags build community and promoted authors and books are a small and select group. Young people use Booktok to provide recommendations, explore reader experience and emotional response, generate reader community and identity, discuss fictional characters and places, develop and promote writing, and discuss personal library management and being a reader in the family. Libraries' reader advisory services for young people can use these findings to employ language of the discourse community for insider signposting of the library's physical space and give greater primacy to the emotional response evoked by books and reading.     

Socio-emotional well-being benefits of yoga for atypically developing children

This pilot study set out to examine the impact of a regular practice of yoga within schooling curricular hours on the well-being and behaviors of atypically developing children. The design of this study was based on a qualitative hermeneutic-phenomenology approach. The intervention consisted of 5 weekly sessions of yoga of 15 minutes each and composed of breathing techniques (pranayama), postures (pranayama), and lying down relaxation exercises (yoga nidra). The research site was a special needs school in South Auckland, New Zealand in an area characterized by poor socio-economic living conditions with a composite range of different ethnic groups. The findings of this study suggest that a regular practice of yoga can have numerous benefits for children on the Autism Spectrum Disorder (ASD). They found yoga to be a fun-filled and engaging experience. They improved significantly in their flexibility levels and general physical health. Emotionally, the children reported that the breathing techniques allowed them to feel calmer and more relaxed. The children felt that incorporating a regular practice of yoga within formal curriculum will be another effective tool to enhancing their overall health and well-being.