题目：遗传改造细菌基因组的策略：基因定点突变、基因失活和基因过表达

With the availability of the whole genome sequence of Escherichia coli or Corynebacterium glutamicum, strategies for directed DNA manipulation have developed rapidly. DNA manipulation plays an important role in understanding the function of genes and in constructing novel engineering bacteria according to requirement. DNA manipulation involves modifying the autologous genes and expressing the heterogenous genes. Two alternative approaches, using electroporation linear DNA or recombinant suicide plasmid, allow a wide variety of DNA manipulation. However, the over-expression of the desired gene is generally executed via plasmid-mediation. The current review summarizes the common strategies used for genetically modifying E. coli and C. glutamicum genomes, and discusses the technical problem of multi-layered DNA manipulation. Strategies for gene over-expression via integrating into genome are proposed. This review is intended to be an accessible introduction to DNA manipulation within the bacterial genome for novices and a source of the latest experimental information for experienced investigators.     

Emotional intelligence and academic attainment of British secondary school children: a cross-sectional survey

Trait emotional intelligence (trait EI) covers a wide range of self-perceived skills and personality dispositions such as motivation, confidence, optimism, peer relations and coping with stress. In the last few years, there has been a growing awareness that social and emotional factors play an important part in students’ academic success and it has been claimed that those with high scores on a trait EI measure perform better. This research investigated whether scores on a questionnaire measure of trait EI were related to school performance in a sample of British pupils. Results showed that high performing students had higher trait EI scores than low performing students and that some aspects of trait EI (motivation and low impulsivity) as well as total trait EI were significant predictors of academic achievement after controlling for prior attainment at school. Therefore, initiatives to develop the emotional and social abilities of schoolchildren might be worthwhile and more effective than concentrating solely on teaching and curriculum activities.     

An associative account of Orthographic Satiation in Chinese characters

Prolonged inspection of a Chinese character induces a feeling of uncertainty of the character, a phenomenon named as Orthographic Satiation. However, there has not been direct evidence showing that such satiation does occur at the orthographic level. To investigate whether Chinese satiation occurs at the orthographic level or at other levels, the present study applies a speeded repetition priming paradigm to Chinese, which has been successfully used to examine Semantic Satiation in English. When judging whether a category name matched an exemplar word, participants showed gradually slower responses as the character standing for the category name was repeated, indicating the occurrence of satiation. However, repetition of a character without accessing its meaning, or repeated access to its meaning without repeating the character itself, did not elicit satiation. The results suggest Orthographic Satiation occurs not at the orthographic level, but at a level associating orthography and meaning during Chinese character reading.     

Going Deep into Mechanisms for Moral Reasoning Growth: How Deep Learning Approaches Affect Moral Reasoning Development for First-year Students

The purpose of this paper was to determine the effects of deep approaches to learning on the moral reasoning development of 1,457 first-year students across 19 institutions. Results showed a modest positive relationship between our measures of deep approaches to learning and moral reasoning at the end of the first year of college even after controlling for precollege moral reasoning. After accounting for a host of demographic and relevant student characteristics and for the natural clustering of students, we found that the integrative learning subscale, which captures students’ participation in activities designed to integrate information from varied sources and diverse perspectives, positively affected moral reasoning among first-year students. Implications for researchers and practitioners are discussed.     

Auditory perceptual processing in reading disabled children

One hundred and three children attending Learning Assistance Centres due to reading difficulties and one hundred and three matched, average readers were administered a battery of auditory perceptual processing tasks. The battery was composed of auditory analysis and synthesis, auditory sequential memory, auditory discrimination, and phonemic segmentation tasks. A principal components analysis yielded four factors. These were determined to be advanced phonological awareness, sequential memory, discrimination, and simple phonological awareness. Discriminant analyses, using the factor scores, indicated that three of the four factors were able to discriminate between the able and disabled readers. Most notable among these was advanced phonological awareness. Auditory discrimination could not discriminate between the groups. The results suggest that there may not be one underlying phonological ability implicated in successful reading acquisition. Furthermore, it is clear that two levels of phonological awareness exist and that screening and diagnostic instruments should address both in order to have predictive validity.     

Towards Building Direct Educational Partnership: The Foundation of Shanxi University in 1902

The foundation of Shanxi University is a prime example of the collaborative efforts in higher education between the Chinese and British in late Qing China (1842–1912). Both sides made compromises, with the Chinese adapting their ideas of educational sovereignty, and the British agreeing to work under the supervision of the local government. Such a collaboration was made possible by the individual personalities of the two founders and their visions for China’s higher education. The dual structure of the university, with one department to teach Chinese learning and the other to teach Western learning, showed that, at a local and personal level, these officials and missionaries opted for direct cooperation, despite the myriad changes and upheavals following the Boxer Movement. In addition, by allowing foreign missionaries into critical roles in a government university, the principle of ti-yong dualism was gradually being adapted. The formation of Shanxi University not only demonstrates the dynamics of the Sino-Western educational relationship and the roles individuals played in it, but also stimulates reflection on China’s contemporary cross-border partnerships in higher education