Fertilization in flowering plants

After the pollen grain reaches the stigma through outsourced agents (pollinators), the next step before fertilization is to select the right type of pollen. Similar to a marriage in human beings, fiowering plants also have evolved elaborate screening process to select the right pollen grains and to reject the wrong ones. Even after initial screening for the right pollen, the pistil imposes a tough competition amongst them, comparable to a swayamvara of Indian mythology, to select the best available pollen. Flowering plants have evolved into a matriarchal society. The selection of the male partner is totally the prerogative of the mother (pistil); the boy (pollen grain) and the girl (ovule) has no say in this selection.     

Molecularly imprinted polymers as the extracted sorbents of clenbuterol ahead of liquid chromatographic determination

A pre-treatment methodology for clenbuterol hydrochloride (CLEN) isolation and enrichment in a complex matrix environment was developed through exploiting molecularly imprinted polymers (MIPs). CLEN-imprinted polymers were synthesized by the combined use of ally-β-cyclodextrin (ally-β-CD) and methacrylic acid (MAA), allyl-β-CD and acrylonitrile (AN), and allyl-β-CD and methyl methacrylate (MMA) as the binary functional monomers. MAA-linked allyl-β-CD MIPs (M-MAA) were characterized by Fourier transform-infrared (FT-IR) spectroscopy and a scanning electron microscope (SEM). Based upon the results, M-MAA polymers generally proved to be an excellent selective extraction compared to its references: AN-linked allyl-β-CD MIPs (M-AN) and MMA-linked allyl-β-CD MIPs (M-MMA). M-MAA polymers were eventually chosen to run through a molecularly imprinted solid-phase extraction (MISPE) micro-column to enrich CLEN residues spiked in pig livers. A high recovery was achieved, ranging from 91.03% to 96.76% with relative standard deviation (RSD) ≤4.45%.     

In vitro remineralization of hybrid layers using biomimetic analogs

Resin-dentin bond degradation is a major cause of restoration failures. The major aim of the current study was to evaluate the impact of a remineralization medium on collagen matrices of hybrid layers of three different adhesive resins using nanotechnology methods. Coronal dentin surfaces were prepared from freshly extracted premolars and bonded to composite resin using three adhesive resins (FluoroBond II, Xeno-III-Bond, and iBond). From each tooth, two central slabs were selected for the study. The slabs used as controls were immersed in a simulated body fluid (SBF). The experimental slabs were immersed in a Portland cement-based remineralization medium that contained two biomimetic analogs (biomineralization medium (BRM)). Eight slabs per group were retrieved after 1, 2, 3, and 4 months, respectively and immersed in Rhodamine B for 24 h. Confocal laser scanning microscopy was used to evaluate the permeability of hybrid layers to Rhodamine B. Data were analyzed by analysis of variance (ANOVA) and Tukey’s honest significant difference (HSD) tests. After four months, all BRM specimens exhibited a significantly smaller fluorescent area than SBF specimens, indicating a remineralization of the hybrid layer (P≤0.05). A clinically applicable biomimetic remineralization delivery system could potentially slow down bond degradation.     

A study of airfoil parameterization,modeling, and optimization based on the computational fluid dynamics method

An excellent airfoil with a high lift-to-drag ratio may decrease oil consumption and enhance the voyage. Based on NACA 0012, an improved airfoil is explored in this paper. The class/shape function transformation has been proved to be a good method for airfoil parameterization, and in this paper it is modified to improve imitation accuracy. The computational fluid dynamics method is applied to obtain numerically the aerodynamic parameters of the parameterized airfoil, and the result is proved credible by comparison with available experimental data in the open literature. A polynomial-based response surface model and the uniform Latin hypercube sampling method are employed to decrease computational cost. Finally, the nonlinear programming by quadratic Lagrangian method is utilized to modify the multi-island genetic algorithm, which has an improved optimization effect than the method used on its own. The obtained result shows that the modified class/shape function transformation method produces a better imitation of an airfoil in the nose and tail regions than the original method, and that it will satisfy the tolerance zone of the model in a wind tunnel. The response surface model based on the uniform Latin hypercube sampling method gives an accurate prediction of the lift-to-drag ratio with changes in the design variables. The numerical result of the flow around the airfoil shows reasonable agreement with the experimental data graphically and quantitatively. Ultimately, an airfoil with better capacity than the original one is acquired using the multi-island genetic algorithm based nonlinear programming by quadratic Lagrangian optimization method. The pressure contours and lift-to-drag ratio along with the attack angle have been compared with those of the original airfoil, and the results demonstrate the strength of the optimized airfoil. The process for exploring an improved airfoil through parameterization to optimization is worth referencing in future work.     

Chinese middle school teachers’ preferences regarding performance evaluation measures

Teacher performance evaluation currently is receiving unprecedented attention from policy makers, scholars, and practitioners worldwide. This study is one of the few studies of teacher perceptions regarding teacher performance measures that focus on China. We employed a quantitative dominant mixed research design to investigate Chinese teachers’ preferences for teacher performance measures and the possible reasons for their preferences. Considering the quantitative results, the surveyed Chinese teachers identified fulfillment of additional roles, collaboration with faculty and staff, and students’ test scores as the most important measures for performance evaluation. Qualitatively, the most important measures that the surveyed teachers reported were the teacher’s morality, workload, and students’ test scores.     

Self-reported symptoms and risk factors for digital ischaemia among international world-class beach volleyball players

The prevalence of ischaemia-related symptoms is remarkably high among elite indoor volleyball players. Since the exposure to sport-specific demands may be higher in beach volleyball compared to indoor volleyball, the aim of this study was to assess the prevalence of ischaemia-related symptoms and associated risk factors among world-class beach volleyball players. Therefore, a questionnaire survey was performed among beach volleyball players active during the 2013 Grand Slam Beach Volleyball in the Netherlands. In total, 60 of the 128 beach volleyball players (47%) participated: 26 males and 34 females from 17 countries. The self-reported prevalence of cold or blue or pale digits in the dominant hand during or immediately after practice or competition was 38% (n = 23). Two risk factors were independently associated with symptoms of blue or pale digits: more than 14 years playing volleyball (odds ratio (OR) 4.42, 90% confidence interval (90% CI) 1.30–15.07) and sex (female) (OR 4.62, 90% CI 1.15–18.57). In conclusion, the prevalence of symptoms associated with digital ischaemia is high among international world-class beach volleyball players. Female sex and the length of the volleyball career were independently associated with an increased risk of ischaemia-related symptoms. The high prevalence of these seemingly innocuous symptoms and possible associated risk factors warrant regular monitoring since early detection can potentially prevent thromboembolic complications and irreversible tissue damage.     

Do karate fighters use situational probability information to improve decision-making performance during on-mat tasks?

This study examined the contribution of situational probability information to the perceptual and cognitive processes underlying decision-making behaviour during in situ Karate performance. Experts and novices were required to make decisions about various attacks in different fight scenarios. The order in which the fight situations were presented was modified in order to provide advanced probability information and identify whether fighters were able to use the latter information to make better decisions. Specifically, one of the attacks was repeated every four actions. Results revealed that experts were more accurate and faster than their less skilled counterparts to block and counterattack the opponent. The experts picked up the occurrence of the attack pattern after the fifth repetition whereas novices did not. This enabled experts to improve decision time and decision accuracy. Findings suggest that such superiority could stem from the perceptual and cognitive skills possessed by the experts, thus giving them the opportunity to recognise a situation more easily. This was reinforced by gaze behaviour which demonstrated that experts used a more efficient search strategy involving fewer fixations of longer duration on a lesser number of areas relative to the novices. Moreover, experts generated more refined karate-specific knowledge structures compared with the novices.     

Mediating effects of resistance training skill competency on health-related fitness and physical activity: the ATLAS cluster randomised controlled trial

The purpose of this study was to examine the mediating effect of resistance training skill competency on percentage of body fat, muscular fitness and physical activity among a sample of adolescent boys participating in a school-based obesity prevention intervention. Participants were 361 adolescent boys taking part in the Active Teen Leaders Avoiding Screen-time (ATLAS) cluster randomised controlled trial: a school-based program targeting the health behaviours of economically disadvantaged adolescent males considered “at-risk” of obesity. Body fat percentage (bioelectrical impedance), muscular fitness (hand grip dynamometry and push-ups), physical activity (accelerometry) and resistance training skill competency were assessed at baseline and post-intervention (i.e., 8 months). Three separate multi-level mediation models were analysed to investigate the potential mediating effects of resistance training skill competency on each of the study outcomes using a product-of-coefficients test. Analyses followed the intention-to-treat principle. The intervention had a significant impact on the resistance training skill competency of the boys, and improvements in skill competency significantly mediated the effect of the intervention on percentage of body fat and the combined muscular fitness score. No significant mediated effects were found for physical activity. Improving resistance training skill competency may be an effective strategy for achieving improvements in body composition and muscular fitness in adolescent boys.     

Dribbling determinants in sub-elite youth soccer players

Dribbling speed in soccer is considered critical to the outcome of the game and can assist in the talent identification process. However, little is known about the biomechanics of this skill. By means of a motion capture system, we aimed to quantitatively investigate the determinants of effective dribbling skill in a group of 10 Under-13 sub-elite players, divided by the median-split technique according to their dribbling test time (faster and slower groups). Foot-ball contacts cadence, centre of mass (CoM), ranges of motion (RoM), velocity and acceleration, as well as stride length, cadence and variability were computed. Hip and knee joint RoMs were also considered. Faster players, as compared to slower players, showed a 30% higher foot-ball cadence (3.0 ± 0.1 vs. 2.3 ± 0.2 contacts · s^?1, P < 0.01); reduced CoM mediolateral (0.91 ± 0.05 vs. 1.14 ± 0.16 m, P < 0.05) and vertical (0.19 ± 0.01 vs. 0.25 ± 0.03 m, P < 0.05) RoMs; higher right stride cadence (+20%, P < 0.05) with lower variability (P < 0.05); reduced hip and knee flexion RoMs (P < 0.05). In conclusion, faster players are able to run with the ball through a shorter path in a more economical way. To effectively develop dribbling skill, coaches are encouraged to design specific practices where high stride frequency and narrow run trajectories are required.