Integration of spatial relationships and temporal relationships in humans

Three experiments tested human participants on a two-dimensional, computer, landmark-based search task to assess the integration of independently acquired spatial and temporal relationships. Experiment 1 showed that A-B spatial training followed by B-outcome spatial training resulted in spatial integration in such a way that A was effectively associated with the outcome. Experiment 2 showed that A-B spatial and temporal training followed by B-outcome spatial and temporal training resulted in integration that created both spatial and temporal relationships between A and the outcome. Experiment 3 refuted an alternative explanation, one that is based on decision-making speed, to the temporal-integration strategy that was suggested by Experiment 2. These results replicate in humans the observations regarding spatial integration made by Sawa, Leising, and Blaisdell (2005) using a spatial-search task with pigeons, and they extend those observations to temporal integration.     

On-chip three-dimensional tumor spheroid formation and pump-less perfusion culture using gravity-driven cell aggregation and balanced droplet dispensing

This paper presents a spheroid chip in which three-dimensional (3D) tumor spheroids are not only formed by gravity-driven cell aggregation but also cultured at the perfusion rates controlled by balanced droplet dispensing without fluidic pumps. The previous spheroid chips require additional off-chip processes of spheroid formation and extraction as well as bulky components of fluidic pumps. However, the present spheroid chip, where autonomous medium droplet dispensers are integrated on a well array, achieves the on-chip 3D tumor spheroid formation and perfusion culture using simple structure without bulky fluidic pumps. In the experimental study, we demonstrated that the spheroid chip successfully forms 3D tumor spheroids in the wide diameter range of 220 μm–3.2 mm (uniformity > 90%) using H358, H23, and A549 non-small cell lung cancer cells. At the pump-less perfusion culture (Q = 0.1–0.3 μl/min) of spheroids, the number of H358 cells in the spheroid increased up to 50% from the static culture (Q = 0 μl/min) and the viability of the cultured cells also increased about 10%. Therefore, we experimentally verified that the perfusion environment created by the spheroid chip offers a favourable condition to the spheroids with high increase rate and viability. The present chip achieves on-chip 3D tumor spheroid formation and pump-less perfusion culture with simple structure, thereby exhibiting potential for use in integrated in-vivo-like cell culture systems.     

Theory development and convergence of human resource fields: Implications for human performance technology

This study examines major theory developments in human resource (HR) fields and discusses implications for human performance technology (HPT). Differentiated HR fields are converging to improve organizational performance through knowledge‐based innovations. Ruona and Gibson (2004) made a similar observation and analyzed the historical evolution and convergence of three HR‐related fields: human resource management (HRM), human resource development (HRD), and organization development (OD). A field left out in their analysis is HPT. Many learning professionals recognize HPT as a more comprehensive approach to improving organizational performance issues (Molenda & Pershing, 2008; Pershing, 2006). However, little research has been done to advance the theory development of HPT and discuss its relationships within HR fields. This study adds a new perspective to that of Ruona and Gibson's historical analysis of HR fields by examining convergence issues from a theory development perspective.     

Timing of triggering in relation to the cardiac cycle in nonelite rifle shooters

This study investigated the timing of the trigger pull in relation to the cardiac cycle during air rifle shooting. Electrocardiogram) was recorded from 20 male nonelite rifle shooters in two separate testing sessions. The testing was conducted at an indoor shooting range using an optoelectronic shooting system. Each participant fired a shot in the standing position at a distance of 10 m from the target. The results showed that compared to random triggering, the shooters fired more often during the phase of 10-50% of the R wave-to-R wave (R-R) interval and less often during the phase of 50-90%. With regard to performance accuracy, the participants exhibited average or above average performance, when the shot occurred in the beginning (0-50%) and in the end (70-99%) of the R-R interval. The less optimal range for the triggering was located within the 50-70% phase of the R-R interval. The length of R-R interval (i.e., heart rate) did not affect the relation of shot placement within the cardiac cycle to the accuracy of shooting performance. The present results extend previous findings by showing that in air rile shooting the optimum firing time within the cardiac cycle may be located in the systolic phase. It is argued that the systole-diastole dichotomy is not a sufficiently accurate way to investigate the effects of shot placement in the cardiac cycle. Further research is needed to examine the extent to which the relationship between the timing of triggering within the cardiac cycle and shooting performance depends a shooter's skill level.     

专业元数据词表研究

因特网已成为全球性的信息环境，我们可以跨越时空获取信息资源。然而，当用户使用自身社区的术语和元数据方案来检索信息时，现在的因特网技术还没有成熟到能提供专业信息设施。元数据担任很重要的角色来帮助实现社区导向的信息环境。本文进行了两项案例研究，包括建立专业主题词表(用于图书馆和图书馆信息科学资源主题网关(LIS)的核心主题词表)和用于地方社区门户网站的主题词表。从这两个案例可以看出小型的主题词表对专业服务是非常有用的，并且维护工作对词表的建立和应用是至关紧要的。为了在网络上建立专业信息环境，我们必须解决对元数据方案来说相悖的两个需求——社区中的专业化(或者本地化)和社区之间的互操作。这篇文章提供了一个概念模型来理解解决这个问题所要涉及的诸多方面。     

Review Article: Recent advancements in optofluidic flow cytometer

There is an increasing need to develop optofluidic flow cytometers. Optofluidics, where optics and microfluidics work together to create novel functionalities on a small chip, holds great promise for lab-on-a-chip flow cytometry. The development of a low-cost, compact, handheld flow cytometer and microfluorescence-activated cell sorter system could have a significant impact on the field of point-of-care diagnostics, improving health care in, for example, underserved areas of Africa and Asia, that struggle with epidemics such as HIV∕AIDS. In this paper, we review recent advancements in microfluidics, on-chip optics, novel detection architectures, and integrated sorting mechanisms.     

A Comparison of Label Switching Algorithms in the Context of Growth Mixture Models

Simulation studies involving mixture models inevitably aggregate parameter estimates and other output across numerous replications. A primary issue that arises in these methodological investigations is label switching. The current study compares several label switching corrections that are commonly used when dealing with mixture models. A growth mixture model is used in this simulation study, and the design crosses three manipulated variables—number of latent classes, latent class probabilities, and class separation, yielding a total of 18 conditions. Within each of these conditions, the accuracy of a priori identifiability constraints, a priori training of the algorithm, and four post hoc algorithms developed by Tueller et al.; Cho; Stephens; and Rodriguez and Walker are tested to determine their classification accuracy. Findings reveal that, of all a priori methods, training of the algorithm leads to the most accurate classification under all conditions. In a case where an a priori algorithm is not selected, Rodriguez and Walker’s algorithm is an excellent choice if interested specifically in aggregating class output without consideration as to whether the classes are accurately ordered. Using any of the post hoc algorithms tested yields improvement over baseline accuracy and is most effective under two-class models when class separation is high. This study found that if the class constraint algorithm was used a priori, it should be combined with a post hoc algorithm for accurate classification.