A low cost design and fabrication method for developing a leak proof paper based microfluidic device with customized test zone

This article describes a fabrication process for the generation of a leak proof paper based microfluidic device and a new design strategy for convenient incorporation of externally prepared test zones. Briefly, a negative photolithographic method was used to prepare the device with a partial photoresist layer on the rear of the device to block the leakage of sample. Microscopy and Field Emission Scanning Electron Microscopy data validated the formation of the photoresist layer. The partial layer of photoresist on the device channel limits sample volume to 7 ± 0.2 μl as compared to devices without the partial photoresist layer which requires a larger sample volume of 10 ± 0.1 μl. The design prototype with a customized external test zone exploits the channel protrusions on the UV exposed photoresist treated paper to bridge the externally applied test zone to the sample and absorbent zones. The partially laminated device with an external test zone has a comparatively low wicking speed of 1.8 ± 0.9 mm/min compared to the completely laminated device with an inbuilt test zone (3.3 ± 1.2 mm/min) which extends the reaction time between the analyte and reagents. The efficacy of the prepared device was studied with colorimetric assays for the non-specific detection of protein by tetrabromophenol blue, acid/base with phenolphthalein indicator, and specific detection of proteins using the HRP-DAB chemistry. The prepared device has the potential for leak proof detection of analyte, requires low sample volume, involves reduced cost of production (∼$0.03, excluding reagent and lamination cost), and enables the integration of customized test zones.     

试论杨丽萍原生态歌舞剧的特色——以《藏谜》为例

<藏谜>是即<云南映象>之后杨丽萍推出的又一大型原生态歌舞剧,一经问世就受到了大家的关注.杨丽萍原生态歌舞剧能受到大家的关注,在于它具有与以往歌舞剧不同的三个特色:原汁原味的藏民生活;坚守传统的藏族文化;原生态也需要创新.当然,杨丽萍的原生态歌舞剧也有不尽人意之处,需要我们辨证地看待.     

Prevalence of multiple antibiotic resistance and R-plasmid inEscherichia coli isolates of hospital sewage of Aligarh city in India

R-plasmids that transfer antibiotic resistance are common in the non-pathogenicEscherichia coli of the gastro-intestinal tract of human beings and domestic animals, which inturn may enter into sewage. Therefore we have isolated 30Escherichia coli isolates from hospital sewage of Aligarh city. These isolates were tested for their resistance and sensitivity against 10 antibiotics. 90% isolates showed resistance against ampicillin and sulphamethizole. Of the total 30E. coli isolates 86.6% were resistant to erythromycin and rifampicin but none of them was resistant to kanamycin and streptomycin. Plasmids (mol. wt. 16.5 mega daltons) were isolated from five differentE. coli strains which harboured only a single plasmid and were characterized on the basis of antibiogram. Moreover, the transformation experiments were also performed to confirm the resistant character on the plasmid. We conclude that multiple drug resistance among most of theE. coli isolates is plasmid borne.     

Current/voltage-mode universal filter using FTFNs

A new configuration realizing current/voltage-mode (CM/VM) universal filter using two four terminal floating nullors (FTFN) and four passive components is presented. The current-mode configuration has single input and three outputs and realises simultaneously lowpass (LP), bandpass (BP) and highpass (HP) responses from which allpass (AP) and notch functions can also be implemented. The voltage-mode filter has three inputs and two outputs and can be configured to realise all the five filtering functions. The circuit in current-mode uses grounded capacitors which are ideal for monolithic integration. Besides using a bare minimum number of passive components, the topology enjoys low active and passive sensitivity figures. Experimental and PSPICE simulation results are also included.     

Meeting linguistic challenges in the science classroom: pre-service ESL teachers' strategies

This study explores the perceptions and challenges of Malaysian pre-service teachers teaching science in a second language. A qualitative case study method was used with multiple sources of data to provide insights into the challenges that a group of pre-service teachers faced during their teaching practicum. The pre-service teachers had to overcome various challenges that arose due to factors such as lack of competency in the medium of instruction and the students' varying levels of language ability. In addressing the challenges faced, they employed various strategies, including code switching and mixing, teacher-student collaboration, rephrasing and re-emphasizing, the use of instructional aids, and the use of analogies. The evidence presented in this study suggests that attention should be given to addressing the English language policy in the science teacher education programme.     

Analyzing the Social Networks of High- and Low-Performing Students in Online Discussion Forums

An ego network is an individual’s social netwrk relationships with core members. In this study, the ego network parameters in online discussion spaces of high- and low-performing students were compared. The extent to which students’ ego networks changed over the course were also analyzed. Participation in 7 weeks of online discussions were analyzed for 12 high-performing students and 9 low-performing students. Results suggested that ego networks’ compositions of high- and low-performing students were significantly different. Particularly, high-performing students had denser ego networks and tended to exhibit a higher level of centralities than low-performing students. Results of network visualization indicated that high-performing students increased and kept their networks stable over the course in comparison to low-performing students, who had fragmented networks. Several networks’ change mechanisms for high- and low-performing groups are also identified and discussed.     

The Diffusion and Transmission of Football in the Straits Settlements and Malay States, 1874–1899: Early Inter-Settlement Games and Inter-State Competition Among European Clubs and Teams

Abstract

Since 1786 the British occupied Penang, Malacca, and Singapore known as the Straits Settlements. They began indirect rule in Selangor, Perak, Negri Sembilan, and Pahang to form the Federated Malay States in 1896. Cricket and later, football was played by the military and European clubs like the Singapore Cricket Club, the Penang Cricket Club, Selangor Club Lake Club, and Perak Club. The Chinese and Malay in the Straits formed their clubs along ethnic lines. The outstation Malay States clubs of the Kajang Recreation Club, Klang Club, and Malay States Guides had a mixed team of European and Natives. The football tournaments in Singapore were exclusively for Europeans. While the Rodger Football Cup in Selangor was for various non-European teams located in the many districts around Kuala Lumpur. After the railway lines began linking in land towns of Kuala Lumpur, Taiping to Seremban to their respective ports, football teams travelled to other settlements to play matches, facilitating the growth of inter-settlement matches. It enabled the Federated Malay States to organized the first inter-state league in 1899. The diffusion and transmission of football in the Malay States should be seen in context social and political changes in the Malaya Peninsula and its economic growth.     

Training Transfer and Transfer Motivation: The Influence of Individual,Environmental, Situational,Training Design,and Affective Reaction Factors

The purpose of this study is to examine the effects of individual, environmental, training design, and affective reaction factors on training transfer and transfer motivation. To determine the relationship between these factors and their influence on training transfer and to test the model, the researchers collected data from employees in the Malaysian banking sector. Structural equation modeling with Amos 16 was used to test the model and determine the relationship. The study suggested that training stakeholders should manage the training program effectively. Transfer is maximized when trainees have social support, high performance self‐efficacy, and transfer motivation. Stakeholders (e.g., trainers, trainees, supervisors, and peers) are important to the training transfer process, as are learner readiness, trainee reaction, instrumentality, and training retention. This study revealed that perceived content validity and transfer design work together and influence the trainee's performance self‐efficacy. In other words, if trainers want to improve the performance self‐efficacy level of trainees, they need to explain how the trainee can transfer the learned skills at the workplace and make sure the content of the training is similar to the actual job. The main objective of training programs is to align the employee's expertise with organizational goals. Organizations can achieve their desired objectives only when employees transfer the learned skills on the job. Unfortunately, employees often transfer only a small percentage of skills they have learned in training. To effectively manage their training programs, organizations need to identify and focus on the factors that resist effective training transfer.     

PROCRASTINATION AND THE 2 × 2 ACHIEVEMENT GOAL FRAMEWORK IN MALAYSIAN UNDERGRADUATE STUDENTS

The present study investigated academic procrastination in the context of the 2 × 2 goal achievement theoretical framework within a population of 450 Malaysian undergraduate students, aged 18 to 25 years. Participants completed the Achievement Goal Questionnaire and the Tuckman Procrastination Test. Approach dimensions of both the mastery and performance goal orientations were found to be positively correlated with procrastination, but the avoidance dimensions of the mastery and performance goal orientations were not associated with procrastination. In regression analyses, mastery approach was the strongest predictor of procrastination, suppressing the effect of performance approach. The results suggest that the Western values underlying the achievement goals portrayed by the goal achievement model may not accurately reflect the motivations that are intrinsic to non‐Western individuals. Further research is required to either validate such a tool in non‐Western cultures or, alternatively, investigate alternative factors that may potentially influence procrastination within this demographic.     

萎叶提取物增强5-氟尿嘧啶对结肠癌细胞HT29和HCT116的生长抑制作用

研究目的：探讨萎叶（PB）提取物对5-氟尿嘧啶（5-FU）抑制结肠癌细胞HT29和HCT116生长的影响。研究方法：HT29和HCT116细胞分别给予PB、5-FU以及两种药物联合治疗24小时,应用等效线图法分析PB和5-FU的药效学相互作用,AnnexinV／PI染色法检测HT29和HCT116细胞的凋丁L=情况,高效液相色谱法排除PB和5-FU间任何可能的相互化学作用。重要结论：联合PB,低剂量5-FU可以在短时间内起到细胞毒作用,而单独应用PB或5-FU治疗较联合治疗可以诱导更多细胞发生凋亡。进一步采用等效线图法分析显示PB和5-Fu的联合作用在抑制结肠癌细胞HT29和HCT116的生长中分别体现出协同和拮抗作用。因此可以认为在HT29细胞中,PB使得较低剂量5-FU发挥最大抑制结肠癌细胞生长效果,然而在HCT116细胞中,PB没有显著降低5-FU的药物浓度,说明PB和5-FU的相互作用不仅仅体现在诱导细胞凋亡方面。