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Lamberti F Luni C Zambon A Andrea Serra P Giomo M Elvassore N 《Biomicrofluidics》2012,6(2):24114-2411413
Miniaturization in biological analyses has several advantages, such as sample volume reduction and fast response time. The integration of miniaturized biosensors within lab-on-a-chip setups under flow conditions is highly desirable, not only because it simplifies process handling but also because measurements become more robust and operator-independent. In this work, we study the integration of flow amperometric biosensors within a microfluidic platform when analyte concentration is indirectly measured. As a case study, we used a platinum miniaturized glucose biosensor, where glucose is enzymatically converted to [Formula: see text] that is oxidized at the electrode. The experimental results produced are strongly coupled to a theoretical analysis of fluid dynamic conditions affecting the electrochemical response of the sensor. We verified that the choice of the inlet flow rate is a critical parameter in flow biosensors, because it affects both glucose and [Formula: see text] transport, to and from the electrode. We identify optimal flow rate conditions for accurate sensing at high time resolution. A dimensionless theoretical analysis allows the extension of the results to other sensing systems according to fluid dynamic similarity principles. Furthermore, we developed a microfluidic design that connects a sampling unit to the biosensor, in order to decouple the sampling flow rate from that of the actual measurement. 相似文献
93.
Today’s youth spend a lot of time on the Internet where they meet a multimodal world. The focus in the present study has been on the skills and abilities needed for on-line reading. This study explores reading on the Internet, with pairs of Swedish students aged 10 and 15. The pairs completed tasks on the Internet and these sessions were video-taped. Five main categories of skills and abilities were found: traditional literacy, multimodal literacy, path-finding, IT abilities, and information abilities. The results support earlier research in the field at large, and also add to the literature on on-line reading, in areas such as the crucial need for the ability to spell and knowing web address conventions in English. 相似文献
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Tomas R. Giberson Monica W. Tracey Marguerite T. Harris 《Performance Improvement Quarterly》2006,19(4):43-61
This article explores the use of an evaluation model of learning and development utilizing formative, summative, and confirmative steps, along with a framework for developing evaluation tools aligned with organizational change goals. A case study is presented in which formative, summative, and confirmative evaluations were used to assess materials and learners following implementation of a new performance management process, tools, and supporting training. A specific model for developing evaluation tools and techniques is introduced as a way to integrate the evaluation process with the specific content of a learning and development intervention, as well as link to higher‐order cultural change goals. Results of the case study suggest that evaluation tools can provide evidence of improvement and target areas for further work. Recommendations are provided for the researcher or practitioner interested in applying the same or similar models to evaluation. 相似文献
97.
Gunvor Birkeland Wilhelmsen Monica L. Aanstad Eva Iren B. Leirvik 《Support for Learning》2015,30(2):134-149
This article presents experiences from vision research implemented in education and argues for the need for teachers with visual competence and insight into suitable methods for stimulation and learning. A new type of continuing professional development (CPD) focuses on the role of vision in children's learning and development, the consequences of frequently occurring vision disturbances and related new educational possibilities. Participating teachers undertake a period of practical teaching training in their home community. Two students' cases visualise the need to break out of the narrow definition of visual impairment and become capable of evaluating the visual challenges affecting many pupils and their learning. The two cases presented represent various issues of visual challenges; both reached a better functional level, including better reading, following improved vision after the educational interventions. These results illustrate that vision competence in schools is most useful and ought to be widely accessible. 相似文献
98.
The Italian middle school in a deregulation era: modernity through path-dependency and global models
Monica E. Mincu 《比较教育学》2015,51(3):446-462
In the current context of intensified moves towards educational deregulation, the configuration of the Italian middle school and its relationship to education governance is an interesting case. Historically, it represents a unique example of the successful ‘decision-making' model of the welfarist era. Despite some internal constraints, at the end of the 1970s it was considered a progressive model of schooling for its time. At present, however, internal institutional processes and path-dependencies have rendered its comprehensiveness relatively formalised and weak. A key finding of this article is that ideas, such as the ‘two people’ theory, have played a crucial role in shaping actors' strategies and goals, while references to world models, in particular the English model, have mediated the reception of global policies. Cultural factors, such as the role played by families, are deeply linked to structural issues and inequalities. An ethnographic account depicts a welfarist institution whose internal processes and links to the wider society may be seen as an example of Italian modernity that ‘speaks for itself’. Fieldwork clarifies the role of the teachers in mediating a path-dependent and cultural reproduction pattern. I argue that partial deregulation is a major source of inequalities at the middle school level, engendered by both new and old mechanisms, which further intensifies a selective path in education. This analysis will show how global deregulation trends meet local and enduring path-dependencies. 相似文献
99.
Classroom discussions have become a centerpiece of reform efforts in science education because talk mediates the joint co-constructing of knowledge in science classrooms. Although decades of research underscore the importance of talk in supporting science learning, the science education community continues to grapple with how to support teachers and students in navigating the uncertainty that is associated with doing knowledge building work. To address these challenges, we must examine not just what gets constructed (the scientific ideas), but how knowledge is co-constructed by teachers and students (the process of building those ideas) amidst uncertainty. In this study, we propose a conceptual tool for identifying organizational, epistemic, and interpretive metadiscourse markers (MDMs) in science talk. We highlight how teachers and students use these three types of MDMs as they navigate uncertainty while connecting ideas within and across multiple turns of talk, leveraging resources for knowledge building, and making interpretations about one another's ideas. We conclude with a set of suggestions for how researchers and teachers can utilize this framework to attend to the ways that MDMs index the organizational, epistemic, and interpretive dimensions of uncertainty in the knowledge building process. 相似文献
100.
This research focuses specifically on uncertainty and information seeking in a digital environment. In this research we argue that different types of uncertainty are associated with the information seeking process and that, with the proliferation of new and different search tools, sources and channels, uncertainty, positive/desirable or negative/undesirable, continues to be a significant factor in the search process. Users may feel uncertain at any stage of the information search and retrieval process and uncertainty may remain even after completion of the process resulting in what may be called persistent uncertainty. An online questionnaire was used to collect data from users in the higher education sector. There were three parts to the questionnaire focusing on: information seeking activities, information seeking problems, and access to specific information channels or sources. Quantitative analysis was carried out on the data collected through the online questionnaire. A total of 668 responses were returned from the chosen user categories of academic staff, research staff and research students. This research has shown that there are some information seeking activities and information seeking problems that are the most common causes of uncertainty among significant number of users from different disciplines, age, gender, ICT skills, etc. This is also the case with respect to access to and use of specific information sources/channels, although the degrees of uncertainty in relation are relatively small. Possible implications of this study and further research issues are indicated. 相似文献