Simple surface modification of poly(dimethylsiloxane) for DNA hybridization

Here, we present a simple chemical modification of poly(dimethylsiloxane) (PDMS) by curing a mixture of 2 wt% undecylenic acid (UDA) in PDMS prepolymer on a gold-coated glass slide. This gold slide had been previously pretreated with a self-assembled hydrophilic monolayer of 3-mercaptopropionic acid (MPA). During curing of the UDA∕PDMS prepolymer, the hydrophilic UDA carboxyl moieties diffuses toward the hydrophilic MPA carboxyl moieties on the gold surface. This diffusion of the UDA within the PDMS prepolymer to the surface is a direct result of surface energy minimization. Once completely cured, the PDMS is peeled off the gold substrate, thereby exposing the interfacial carboxyl groups. These groups are then available for subsequent attachment of 5(')-amino terminated DNA oligonucleotides via amide linkages. Our results show that the covalently tethered oligonucleotides can successfully capture fluorescein-labeled complementary oligonucleotides via hybridization, which are visualized using fluorescence microscopy.     

Claiming more: the Increased Voluminosity of Patent Applications and its Determinants

The size of patent applications has doubled over the past two decades, resulting in a dramatic surge in the workload of patent offices all over the world and serious concerns over patent quality standards. The current paper investigates the sources of this inflation in claims and pages for EPO applications. Four hypotheses are quantitatively examined: the diffusion of national drafting practices, the complexity of research activities, the emergence of new sectors, and filing strategies. The results validate the four hypotheses. They reveal major differences across countries in patent drafting styles, especially between Civil and Common Law countries, the latter being characterized by much larger patents. Second, the success of the PCT route is leading to the harmonizing of drafting styles worldwide on the US model. This paper therefore challenges the commonly accepted idea that more claims reflect a broader scope of protection by showing that the size of patents is partly due to institutional changes in the system.     

Time courses of emotions experienced after a mountain ultra-marathon: Does emotional intelligence matter?

This research examined the time courses of emotions in sport settings (anxiety, dejection, anger, happiness, excitement) experienced by mountain ultra-marathon (MUM) runners within the month following a demanding MUM race and the role of emotional intelligence (EI) in these time courses. A six-wave one-month longitudinal design was used with one measurement point within two days before the race to measure EI and five time points within the month following the race to assess emotions experienced among a sample of 29 runners. Results of multilevel growth curve analyses showed significant linear decreases of dejection and anxiety and a significant linear increase of anger. EI was related to the intercept (level at the end of the MUM race) of happiness, excitement and dejection. Moreover the interaction of EI with time was associated with happiness, excitement and anger. This means that high and low emotional intelligent runners exhibited distinct trajectories of emotional intelligence within the month following the MUM race. Indeed, trait-EI appeared to have a protective role against stress process leading to emotional adjustment within the recovery period following an ultra-endurance event. As such, consultants and coaches could conduct specific program over the sport season designed to enhance trait-EI of MUM runners.     

Mechanics of standing and crouching sprint starts

The aim of this study was to compare the kinetic and kinematic parameters of standing and crouch sprint starts. Parallel starts (PS), false starts (FS), jump starts (JS) and crouch starts (3PS) were compared. Eighteen participants performed each start on a force plate and six infrared cameras captured the three-dimensional coordinates of 36 retro-reflective markers. Performance during a five-metre sprint (T_5m) was analysed. Duration of the start phase (T_start), mean values of horizontal and total ground reaction forces (GRFs) (F_{x_mean} and F_{tot_mean}), ratio of force (RF), maximal power (P_max) and kinetic energy (KE) of each limb were calculated. Significant differences were found for T_5m, T_start, KE, P_max, F_{x_mean}, F_{tot_mean} and RF for the crouch start compared to the other starts (P ≤ 0.05). Significant correlations were found between T_5m and T_start (r = 0.59; P ≤ 0.001), and T_5m and P_max, F_{x_mean} and RF (?0.73 ≤ r ≤ ?0.61; P ≤ 0.001). To conclude, the crouch start resulted in the best performance because T_start was shorter, producing greater P_max, F_{x_mean} with a more forward orientation of the resultant force. Greater KE of the trunk in each start condition demonstrated the role of the trunk in generating forward translation of the centre of mass (CM).     

Upper limb and trunk muscle activity patterns during seated and standing cycling

The objective of this study is to clarify the functional roles of upper limb muscles during standing and seated cycling when power output increases. We investigated the activity of seven upper limb and trunk muscles using surface electromyography (EMG). Power outputs ranged from ~100–700 W with a pedalling frequency of 90 revolution per minute. Three-dimensional handle and pedal forces were simultaneously recorded. Using non-negative matrix factorisation, we extracted muscle synergies and we analysed the integrated EMG and EMG temporal patterns. Most of the muscles showed tonic activity that became more phasic as power output increased. Three muscle synergies were identified, associated with (i) torso stabilisation, (ii) compensation/generation of trunk accelerations and (iii) upper body weight support. Synergies were similar for seated and standing positions (Pearson’s r > 0.7), but synergy #2 (biceps brachii, deltoidus and brachioradialis) was shifted forward during the cycle (~7% of cycle). The activity levels of synergy #1 (latissimus dorsi and erector spinae) and synergy #2 increased markedly above ~500 W (i.e., ~+40–70% and +130–190%) and during periods corresponding to ipsi- and contralateral downstrokes, respectively. Our study results suggest that the upper limb and trunk muscles may play important roles in cycling when high power outputs are required.     

Relationships between cohesion, collective efficacy and performance in professional basketball teams: an examination of mediating effects

The main aim of this study was to examine mediating effects in the relationships between cohesion, collective efficacy and performance in professional basketball teams. A secondary aim was to examine the correlates of collective efficacy in a professional sport. A total of 154 French and foreign professional players completed French or English versions of questionnaires about cohesion and collective efficacy. Two composite measures of individual performance were used (pre- and post-performance). Individual-level analyses were performed. Regression analyses supported two mediating relationships with collective efficacy as a mediator of the pre-performance - Group integration-task relationship, and Group integration-task as a mediator of the pre-performance - collective efficacy relationship. Statistical analyses indicated that neither Group integration-task nor collective efficacy was a better mediator in the relationship between pre-performance and the other group variables. Results also revealed positive relationships between three dimensions of cohesion (i.e. Individual attractions to the group-task, Group integration-task, Group integration-social) and collective efficacy. These findings suggest that in professional basketball teams, staff members should look after athletes who perform at a lower or below their usual level because their performances might lead them into a downward cohesion - collective efficacy spiral. Staff members should also develop a high quality of group functioning, both on and off the basketball court, given its relationship with collective efficacy.     

Practicing induction: a generative dance between newcomers and organizations

Research on induction of newcomers is primarily focused on individual processes, such as acquisition of knowledge and socialization in order to create a smooth and frictionless entry period. The interest of our research, however, is the processes that happen on the organizational level. We claim that induction potentially triggers both individual and organizational learning and by drawing on practice-based theory we discuss how the interplay between individual and the organization, what we call a generative dance, ignites both kinds of learning. We focus on and describe the interplay that takes place in particular induction events and analyze the “dance” through the lens of learning. The article concludes with a brief discussion about the implications for practitioners and the challenges and future research prospects we have encountered. We draw on an empirical enquiry in two organizational settings, a Danish management consulting company and a German retail bank.

Development of an automatic and mobile X-ray robot: An innovative approach to medical imaging through mechatronics

The objective of this work is the development of an automatic and mobile X-ray robot that is becoming necessary in a changing environment (aging society, growth of the developing countries, etc.). Specifically, there are three sides to such a project. First, there is the mechanical side, i.e. the design and implementation of motors on every mobile part of an X-ray unit allowing it to move around a standing patient. Then, comes the electronic side, i.e. the use of potentiometers and limit switches as feedback combined with microcontrollers. And last, the algorithm that computes three-dimensional models from two-dimensional X-rays.     

Protective effect of endolithic fungal hyphae on oolitic limestone buildings

This study presents characterizations of weathering forms of the same oolitic limestone from four quarries and eight monuments exposed on various environmental conditions focusing on the waterproofing effect of endolithic organic matter. Patinas were analyzed by X-ray diffraction (XRD), scanning electron microscopy with energy dispersive X-ray spectrometry (SEM-EDX), capillarity coefficient through weathered and unweathered sides, gypsum content and porous network morphology by epoxy resin molding. Study of weathering forms on old quarries indicates that lichens colonization (Verrucaria nigrescens and Caloplaca aurantia) can fill the superficial porous network with a dense network of lichenised fungal hyphae. Capillary coefficient measurement on natural and calcinated samples showed that endolithic organic matter can waterproof the stone and could act as a sulfate contamination barrier. Similar endolithic organic layer due to ancient lichens growth are found on some antique monuments of the Nîmes downtown and could explain their well-preserved state, unlike decayed 19th century churches that were never colonized by lichens.