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Patric Wallin Carl Zandén Bj?rn Carlberg Nina Hellstr?m Erkenstam Johan Liu Julie Gold 《Biomicrofluidics》2012,6(2)
The properties of a cell’s microenvironment are one of the main driving forces in cellular fate processes and phenotype expression invivo. The ability to create controlled cell microenvironments invitro becomes increasingly important for studying or controlling phenotype expression in tissue engineering and drug discovery applications. This includes the capability to modify material surface properties within well-defined liquid environments in cell culture systems. One successful approach to mimic extra cellular matrix is with porous electrospun polymer fiber scaffolds, while microfluidic networks have been shown to efficiently generate spatially and temporally defined liquid microenvironments. Here, a method to integrate electrospun fibers with microfluidic networks was developed in order to form complex cell microenvironments with the capability to vary relevant parameters. Spatially defined regions of electrospun fibers of both aligned and random orientation were patterned on glass substrates that were irreversibly bonded to microfluidic networks produced in poly-dimethyl-siloxane. Concentration gradients obtained in the fiber containing channels were characterized experimentally and compared with values obtained by computational fluid dynamic simulations. Velocity and shear stress profiles, as well as vortex formation, were calculated to evaluate the influence of fiber pads on fluidic properties. The suitability of the system to support cell attachment and growth was demonstrated with a fibroblast cell line. The potential of the platform was further verified by a functional investigation of neural stem cell alignment in response to orientation of electrospun fibers versus a microfluidic generated chemoattractant gradient of stromal cell-derived factor 1 alpha. The described method is a competitive strategy to create complex microenvironments invitro that allow detailed studies on the interplay of topography, substrate surface properties, and soluble microenvironment on cellular fate processes. 相似文献
226.
Amanda E. Guyer Erin B. McClure-Tone Nina D. Shiffrin Daniel S. Pine Eric E. Nelson 《Child development》2009,80(4):1000-1015
Neural correlates of social-cognition were assessed in 9- to- 17-year-olds ( N = 34) using functional magnetic resonance imaging. Participants appraised how unfamiliar peers they had previously identified as being of high or low interest would evaluate them for an anticipated online chat session. Differential age- and sex-related activation patterns emerged in several regions previously implicated in affective processing. These included the ventral striatum, hippocampus, hypothalamus, and insula. In general, activation patterns shifted with age in older relative to younger females but showed no association with age in males. Relating these neural response patterns to changes in adolescent social-cognition enriches theories of adolescent social development through enhanced neurobiological understanding of social behavior. 相似文献
227.
Sibling relationship quality and social understanding (second-order false belief, conflict interpretation, and narrative conflict perspective references) were examined as unique and interactive correlates of sibling conflict behavior in 62 dyads (older M age = 8.39 years and younger M age = 6.06 years). High-quality relationships were associated with positive conflict processes. Younger siblings' second-order false belief scores were negatively associated with constructive conflict strategies, and older siblings' narrative self-referential focus was negatively associated with compromise. Associations between younger children's social understanding (conflict interpretation and narrative perspective references) and siblings' dyadic conflict behavior were moderated by relationship quality. Results suggest that links between social understanding and conflict behavior should be considered in conjunction with the quality of children's relationships. 相似文献
228.
Nina Witoszek 《Environmental Education Research》2018,24(6):831-844
The article compares how the UN-initiated education for sustainable development (ESD) has fared in three seemingly dissimilar countries: Norway, a wealthy, ‘post-materialist’ liberal democracy, Ghana, a developing democratic country, and China, a fast catching-up, centrally- steered economy. The study – based on an analysis of national ESD programmes, schoolbooks and qualitative interviews with teachers and students – discusses some of the pivotal reasons for the decline in ESD schooling in all three countries. It also explores surprising ‘archipelagos of pedagogical innovation’, as shown by one of the high schools in Ghana. Our conclusions are that, apart from specific, cultural and political contexts which influence ESD, students’ socio-environmental literacy in the examined countries has been affected by an ever more pervasive competitive and neoliberal mindset. Further, in all three cases, the agenda of ‘sustainable development’ suffers from a ‘narrative and mythical deficit’: a lack of a mobilizing story, the absence of which reduces the attractiveness of sustainability ideals and inhibits their empowering potential. 相似文献
229.
Richard Quentin Shin Lance Christian Smith Kristopher Michael Goodrich Nina Daniele LaRosa 《International journal for the advancement of counseling》2011,33(2):113-126
Extant research has neglected to explore the diversity representation of counselor education programs. This pilot study sought
to examine the extent to which CACREP-accredited master’s-level programs attend to the representation of people of color,
individuals with dis/abilities, lesbian, gay, bisexual (LGB), and transgender persons within admissions, enrollment, and graduation
data. The findings indicated little consistency in the maintenance of students’ demographic characteristics among participating
programs. The authors contend that counseling programs need to critically analyze their policies and procedures to ensure
a responsive milieu for students from diverse backgrounds. Implications for the counseling field are presented. 相似文献
230.
ON THE EPISTEMOLOGICAL PRESUPPOSITIONS OF REFLECTIVE ACTIVITIES 总被引:1,自引:0,他引:1
Nina Bonderup Dohn 《Educational theory》2011,61(6):671-708
Reflection is an ambiguous buzzword in contemporary educational and professional settings. Work has been done to clarify the concept theoretically, but a gap remains between such clarifications and actual reflective activities in educational and work‐related practices. Reflective activities embody epistemological presuppositions about the nature of competence, knowledge, and learning, and about the relation between thinking, communicating, and acting. In this article, Nina Bonderup Dohn identifies the epistemological presuppositions of two paradigm cases of reflection (“solitaire reflection” and “communicative reflection”) and assesses these against a view of knowledge, competence, learning, and action inspired by the Scandinavian interpretation of Ludwig Wittgenstein's philosophy as well as by insights from phenomenology and situated learning. The outcome of Dohn's assessment is that the presuppositions of the paradigm cases are misguided and, therefore, so are the reflective activities. The problems and pitfalls that result from this situation are identified and illustrated with empirical examples. Dohn concludes by suggesting an alternative paradigm: “situated reflection.” 相似文献