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121.
Paolo Guidoni 《International Journal of Science Education》2013,35(2):133-140
This article presents figures which show that environmental education is part of the curriculum for different subjects in West German schools. Criticism is made in the following areas: still too little time is used for environmental education; a lack of innovation in using new methods; a lack of adequate scope for teachers to shape their own teaching in a non‐disciplinary, action‐ and situation‐oriented way. 相似文献
122.
Paolo Stievano Silvia Michetti Shawn M. McClintock Gabriel Levi Teresa Gloria Scalisi 《Reading and writing》2016,29(7):1497-1510
Handwriting is a complex activity that involves continuous interaction between lowerlevel perceptual-motor and higher-level cognitive processes. All handwriting models describe involvement of executive functions (EF) in handwriting development. Particular EF domains associated with handwriting include maintenance of information in working memory, inhibition of prepotent responses, and shifting and sustaining of attention. Generativity, an EF domain that has not been extensively studied, may play a role in the acquisition and automation of handwriting. We hypothesized that neuropsychological performances and handwriting fluency would be mutually associated, and that visuospatial generativity could have a specific role in handwriting speed. We enrolled 96 children who completed a comprehensive neuropsychological battery. The study found that handwriting speed was associated with visuospatial fluency, which involves behavioural execution and initiation. In learning and performing handwriting, there is an inherent non-exclusive role of fine motor skills. 相似文献
123.
Objective
The reliability of child witness testimony in sexual abuse cases is often controversial, and few tools are available. Criteria-Based Content Analysis (CBCA) is a widely used instrument for evaluating psychological credibility in cases of suspected child sexual abuse. Only few studies have evaluated CBCA scores in children suspected of being sexually abused. We designed this study to investigate the reliability of CBCA in discriminating allegations of child sexual abuse during court hearings, by comparing CBCA results with the court's final, unappealable sentence. We then investigated whether CBCA scores correlated with age, and whether some criteria were better than others in distinguishing cases of confirmed and unconfirmed abuse.Methods
From a pool of 487 child sexual abuse cases, confirmed and unconfirmed cases were selected using various criteria including child IQ ≥ 70, agreement between the final trial outcome and the opinion of 3 experts, presence of at least 1 independent validating informative component in cases of confirmed abuse, and absence of suggestive questions during the child's testimonies. This screening yielded a study sample of 60 confirmed and 49 unconfirmed cases. The 14 item version of CBCA was applied to child witness testimony by 2 expert raters.Results
Of the 14 criteria tested, 12 achieved satisfactory inter-rater agreement (Maxwell's Random Error). Analyses of covariance, with case group (confirmed vs. unconfirmed) and gender as independent variables and age as a covariate, showed no main effect of gender. Analyses of the interaction showed that the simple effects of abuse were significant in both sex. Nine CBCA criteria were satisfied more often among confirmed than unconfirmed cases; seven criteria increased with age.Conclusion
CBCA scores distinguish between confirmed and unconfirmed cases. The criteria that distinguish best between the 2 groups are Quantity of Details, Interactions, and Subjective Experience. CBCA scores correlate positively with age, and independently from abuse; all the criteria test except 2 (Unusual Details and Misunderstood Details) increase with age. The agreement rate could be increased by merging criteria Unusual and Superfluous details that achieve a low inter-rater agreement when investigated separately.Practice implication
Given its ability to distinguish between confirmed and unconfirmed cases of suspected child abuse, the CBCA could be a useful tool for expert opinion. Because our strict selection criteria make it difficult to generalize our results, further studies should investigate whether the CBCA is equally useful in the cases we excluded from our study (for example mental retardation). 相似文献124.
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126.
More than 20 years ago, electrical impedance spectroscopy (EIS) was proposed as a potential characterization method for flow cytometry. As the setup is comparably simple and the method is label-free, EIS has attracted considerable interest from the research community as a potential alternative to standard optical methods, such as fluorescence-activated cell sorting (FACS). However, until today, FACS remains by and large the laboratory standard with highly developed capabilities and broad use in research and clinical settings. Nevertheless, can EIS still provide a complement or alternative to FACS in specific applications? In this Perspective, we will give an overview of the current state of the art of EIS in terms of technologies and capabilities. We will then describe recent advances in EIS-based flow cytometry, compare the performance to that of FACS methods, and discuss potential prospects of EIS in flow cytometry. 相似文献
127.
Camilla Luni Hope C. Feldman Michela Pozzobon Paolo De Coppi Carl D. Meinhart Nicola Elvassore 《Biomicrofluidics》2010,4(3)
This work presents the development of an array of bioreactors where finely controlled stirring is provided at the microliter scale (100–300 μl). The microliter-bioreactor array is useful for performing protocol optimization in up to 96 parallel experiments of hematopoietic stem cell (HSC) cultures. Exploring a wide range of experimental conditions at the microliter scale minimizes cost and labor. Once the cell culture protocol is optimized, it can be applied to large-scale bioreactors for stem cell production at the clinical level. The controlled stirring inside the wells of a standard 96-well plate is provided by buoyancy-driven thermoconvection. The temperature and velocity fields within the culture volume are determined with numerical simulations. The numerical results are verified with experimental velocity measurements using microparticle image velocimetry (μPIV) and are used to define feasible experimental conditions for stem cell cultures. To test the bioreactor array’s functionality, human umbilical cord blood-derived CD34+ cells were cultured for 7 days at five different stirring conditions (0.24–0.58 μm∕s) in six repeated experiments. Cells were characterized in terms of proliferation, and flow cytometry measurements of viability and CD34 expression. The microliter-bioreactor array demonstrates its ability to support HSC cultures under stirred conditions without adversely affecting the cell behavior. Because of the highly controlled operative conditions, it can be used to explore culture conditions where the mass transport of endogenous and exogenous growth factors is selectively enhanced, and cell suspension provided. While the bioreactor array was developed for culturing HSCs, its application can be extended to other cell types. 相似文献
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129.
Lavinia de Ferri Pier Paolo Lottici Andrea Lorenzi Angelo Montenero Emma Salvioli-Mariani 《Journal of Cultural Heritage》2011,12(4):356
The growing interest in the conservation of historic buildings encourages the development of water-repellent materials and methodologies to consolidate and/or protect stones. Recently, particular attention was devoted to composites of inorganic oxides nanoparticles and hybrid siloxane or silicone polymers. Here we present a study on the water repellence of a thin protective coating obtained through sol-gel process starting from Glymo and Dynasylan 40®, and loading the silicate matrix with nano-sized silica particles (Aeroxide LE1® -Degussa-Evonik). The coatings were applied to limestone, sandstone and granite samples. The silica nanoparticles have been characterized by XRD, the siloxane matrix through micro-Raman spectroscopy, while the surface morphology was examined by SEM. The efficacy of the treatments has been evaluated through static contact angle measurements and capillary water absorption. The optical appearance of the coatings was evaluated by colorimetric measurements. Nanoparticles at suitable concentration gave high values for the static contact angle (up to ∼ 150°), for all stone species even for four months exposure to atmospheric conditions. The coatings, in the water capillary absorption tests, however, behave as expected only for granite even if the long-term water direct contact reduces the hydrophobicity. 相似文献
130.
This paper analyses the market transformation in heritage tourism destinations when excessive tourism demand determines the emergence of a class of excursionists among visitors. Building on the approach of Keane (1997) and Shapiro (1983), some important dimensions of sustainable tourism development are highlighted. The lesser capacity of excursionists to learn the true quality of the tourist goods provides an opportunity for producers to cut back on quality. To serve high quality goods and keep up the reputation of the destination, producers need to gain a mark-up on price that might not be sustained in a competitive market. Hence the decline in ``high-paying' demand segments, increasingly substituted by visitors with lesser quality expectations, has significant consequences on the use and preservation of the heritage. The proposed formulation allows the identification of appropriate policy instruments to reverse this process. 相似文献