Inhibitory and bactericidal activity of the calmodulin antagonist,trifluoperazine, againstMycobacterium avium in vitro and within human monocyte-derived macrophages

The activity of a calmodulin antagonist, trifluoperazine (TFP), was testedin vitro againstMycobacterium avium (ATCC 25291). The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of this compound forM. avium were 20 and 30 μg/ml, respectively. TFP was also found to completely inhibit the growth of 10 isolates ofM. avium (5 patient isolates and 5 environmental isolates) at 30 μg/ml. At near neutral pH (6.8), the MIC of TFP was found to be 20 μg/ml. However, at pH 5.5 (intracellular pH of macrophages), there was a decrease in the inhibitory activity of the compound against this organism. Interestingly, 99.6% ofM. avium within human monocyte-derived macrophages were killed at a drug concentration of 30 μg/ml, which correlates well with the MBC of TFP againstM. avium in vitro. Although the MIC for TFP appears to be higher than that forMycobacterium tuberculosis H₃₇R_v, our studies suggest that calmodulin antagonists might be useful as drugs against infection due toM. avium. It is suggested that administration of TFP in combination with other known drugs may enhance the overall bactericidal effect.     

Negotiating Genre and New Media for STEM News

ABSTRACT

This article—co-authored by a transdisciplinary team of social scientists and journalists in the United States—traces changes to the news landscape in recent decades, and asks: How are legacy media producers grappling with these new realities? As part of a four-year collaboration on young adult news consumption, we take a participatory action research approach to this question, tacking back and forth between newsroom concepts and anthropological ones in pursuit of a synthesis that strengthens both. Starting from anthropological frameworks of participation, the authors argue that broadcast videos typically position their audiences as overhearers rather than interlocutors, while the reverse is true for social media, and that these tendencies shape audience expectations. We find that many audiences have what we call poetic motivations: they are drawn to stories that exemplify their genre. For example, the participatory nature of social media genres translates well to a more candid style that can incorporate live questions and other direct participation. The study reported here focuses on STEM news, but many of the findings apply to news production in general. Our reflective methods can also be applied more widely in the field of journalism to synthesize perspectives from theory and practice.     

Methodology-21: what do we do in the afterward?

This paper asks “after what” and situates qualitative research in the present moment in the midst of various “deaths” and “returns.” With a focus on fleshing out post-qualitative research, it first sketches efforts to discipline qualitative research via standards and rubrics as a part of neoliberal govenmentality and then elaborates what post-qualitative might mean via four exemplars. The first is from Sweden, a focus on relational entangled data analysis in the feminist classroom; the next two exemplars are collaborative studies from Australia at the intersection of Western and Aboriginal knowledge systems; the final exemplar is from Egypt, a feminist post-colonial study of the women’s mosque movement. The paper concludes with a call to “imagine forward” out of troubling a narrow scientificity and enacting an “after” of neoliberalism.     

Engineering an online course: applying the 'secrets' of computer programming to course development

Colleges and universities are increasingly migrating towards utilising the World Wide Web to convey at least part of, and in many cases, their entire curricular offering. Despite this trend there is little support for the professors responsible for translating courses refined over a career in the classroom for delivery via the Web. Teachers who are experts in their subject area and masters of their craft when in a classroom find themselves in the uncomfortable position of having to relearn how to teach in a new environment with little or no support. Development of an online course is, in many significant aspects, analogous to developing a computer product. The procedures and tools utilised in the software engineering field to manage computer software development, therefore, offer promise for developing online courses. This paper explores the potential of one process developed for the software engineering field—the System Development Lifecycle (SDL)—as a tool to effectively design and develop online college courses.     

The 2002 Conference on the Future of School Psychology: Implications for Consultation,Intervention, and Prevention Services

In November 2002, the multisite Conference on the Future of School Psychology was sponsored by major professional associations of school psychologists. Conference goals included achieving consensus on current and future demands for school, conceptualizing the practice of school psychology in the face of diminishing numbers and increasing demand for services, and developing an agenda to use school psychology resources to maximize the benefits to the children and schools. Consultation, intervention, and prevention were major themes in all conference activities. The present article provides an overview of the conference and a summary of issues and outcomes from the conference that relate to educational and psychological consultation services.     

Follow-up to the 2002 Futures Conference: Collaborating to Serve All Children,Families, and Schools

Recommendations of the Thayer, Spring Hill, and Olympia conferences are examined as they relate to ways we can support and make positive contributions to students, families, and schools. In the current issue of the Journal of Educational and Psychological Consultation, Hatzichristou and Lampropoulou (this issue), Meyers, Meyers, and Grogg (this issue), Nastasi (this issue), and Wizda (this issue) share important insights that can guide efforts to implement ideas discussed at the recent 2002 Futures Conference. Their ideas, along with literature on the public health model, are discussed. Points are made about the move to evidence-based interventions, as well as the use of Internet technology to facilitate collaboration and follow-up to the 2002 conference.