Diagnostic accuracy of urinary prostate protein glycosylation profiling in prostatitis diagnosis

Introduction

Although prostatitis is a common male urinary tract infection, clinical diagnosis of prostatitis is difficult. The developmental mechanism of prostatitis is not yet unraveled which led to the elaboration of various biomarkers. As changes in asparagine-linked-(N-)-glycosylation were observed between healthy volunteers (HV), patients with benign prostate hyperplasia and prostate cancer patients, a difference could exist in biochemical parameters and urinary N-glycosylation between HV and prostatitis patients. We therefore investigated if prostatic protein glycosylation could improve the diagnosis of prostatitis.

Materials and methods

Differences in serum and urine biochemical markers and in total urine N-glycosylation profile of prostatic proteins were determined between HV (N = 66) and prostatitis patients (N = 36). Additionally, diagnostic accuracy of significant biochemical markers and changes in N-glycosylation was assessed.

Results

Urinary white blood cell (WBC) count enabled discrimination of HV from prostatitis patients (P < 0.001). Urinary bacteria count allowed for discriminating prostatitis patients from HV (P < 0.001). Total amount of biantennary structures (urinary 2A/MA marker) was significantly lower in prostatitis patients compared to HV (P < 0.001). Combining the urinary 2A/MA marker and urinary WBC count resulted in an AUC of 0.79, 95% confidence interval (CI) = (0.70–0.89) which was significantly better than urinary WBC count (AUC = 0.70, 95% CI = [0.59–0.82], P = 0.042) as isolated test.

Conclusions

We have demonstrated the diagnostic value of urinary N-glycosylation profiling, which shows great potential as biomarker for prostatitis. Further research is required to unravel the developmental course of prostatic inflammation.Key words: diagnostic marker, prostatitis, urinary asparagine-linked glycosylation     

Neural Differences in Bilingual Children's Arithmetic Processing Depending on Language of Instruction

Many children in bilingual regions follow lessons in a language at school (school‐language) that they hardly ever speak at home or in other informal settings. What are the neural effects of this phenomenon? This functional magnetic resonance imaging (fMRI) study investigates the effects of using school‐language on brain activity during a high cognitive task in two groups of French/Dutch bilingual children. Participants were asked to assess the correctness of 3‐operand (e.g., 5 ? 2 = 3) and 4‐operand (e.g., 3 ? 2 = 1 + 4) equations, respectively. Instructions about the task were given either in the children's school‐language or in another language that they only used at home. It was found that although both groups recruited a visuomotor occipitofrontal network in the left hemisphere, the children who performed the task in their school‐language showed less activation than the children who did not, indicating the importance of language of instruction for bilingual children's arithmetic processing.     

The Open College: six months on

What expectations do we make of distance students in terms of the time that they give to study and their study activities each week? Do these expectations match the actual practice of students themselves? By means of postal questionnaires and 14 day diaries, external students of Riverina‐Murray Institute of Higher Education in New South Wales, Australia, informed David Roberts of the difference between Institute assumptions and their own behaviour. The conclusions provide food for thought to educators inclined to recommend ideal study patterns to their students.     

Training Teachers in Mixed‐age Classrooms: effects of a staff development programme

A quasi‐experimental, treatment‐control group investigation was designed to test the effects of the staff development programme Dealing with Mixed‐age Classes. Research findings from mixed‐age glasses, effective teaching and classroom management and organisation were translated into teacher behaviours. Following seven three‐hour workshops teachers in mixed‐aged classes implemented self‐designed plans to increase selected research‐derived teaching behaviours and pupils’ time‐on‐task. Based on pre‐ and post‐training classroom observations, a significant treatment effect was found for pupils’ time‐on‐task levels in mixed‐age classrooms and for teacher behaviours regarding effective instruction, lesson design and execution, classroom organisation and management.     

Free daily newspapers ‐ business models and strategies

Abstract

Free daily newspapers, first introduced in Sweden in 1995, have proved to be much more than a passing phenomenon. By 2002, 80 free daily newspapers were introduced in 26 countries, 60 of them still exist. In thirteen countries free papers are responsible for more than ten percent of the total daily weekday circulation. The total circulation of the 60 free papers is almost 10 million copies, every day more than 20 million people read these papers. The success of the free papers is the result of their efficient cost structure and their ability to reach a new and relatively young audience. When looking more closely at the firms that publish these free newspapers two different models emerge; first, the entrepreneur entering a new market, and second the local or national newspaper firm. In the second model, free papers are launched to prevent other firms entering the market or to counter new firms already in the market. In the long run these obstructive tactics may develop into more positive strategies. Most readers are former ‘non‐readers’ or people who read paid and free newspapers Existing firms are developing strategies to benefit from the growing readership of newspapers created by free papers.     

Discrete element modelling approach to assessment of granular properties in concrete

This paper presents the technological relevance of a concurrent algorithm-based discrete element modelling (DEM) system, HADES. This new system is the successor of SPACE that is limited to spherical grains only. It can realistically simulate the packing of arbitrary-shaped particles up to the fully compacted state. Generation of families of such particles, i.e., generally representing aggregate of fluvial origin and crushed rock, respectively, and the forming way of particulate structure are described. Similarly shaped particles are proposed for simulation of cement paste because of conformity with experimental results obtained by the X-ray tomography method. Technologically relevant territories inside and outside concrete technology are presently explored in this efficient, reliable, and economic way. Some results obtained by this DEM approach are presented.     

Opening up towards children’s languages: enhancing teachers’ tolerant practices towards multilingualism

Mainstream teachers struggle with linguistic diversity, often leading to restricting multilingualism. Scientific research, however, recommends including pupils’ home languages in school. Various qualitative studies have evaluated implementations in schools and indicated possibilities for improving teachers’ attitudes towards multilingualism. This paper evaluates an experimental implementation targeting an increase in tolerance towards multilingualism. The implementation was facilitated by external school coaches and consisted of 3 experimental tools affecting the school. Data originated from 62 Flemish primary schools (of which half were experimental schools) that participated in 3 survey waves (2012 and 2014; 763 teachers completed both waves). We used multilevel regression. We concluded that the implementation leads to higher rates of tolerance. The fulfilment of the basic conditions needed for a successful change was important, and the linguistic diversity of the pupil population and the investment by external school coaches did not affect the tolerant practices of teachers towards multilingualism.