Heat shock protein 90 protects rat mesenchymal stem cells against hypoxia and serum deprivation-induced apoptosis via the PI3K/Akt and ERK1/2 pathways

Mesenchymal stem cell(MSC)transplantation has shown a therapeutic potential to repair the ischemic and infracted myocardium,but the effects are limited by the apoptosis and loss of donor cells in host cardiac microenvironment.The aim of this study is to explore the cytoprotection of heat shock protein 90(Hsp90)against hypoxia and serum deprivation-induced apoptosis and the possible mechanisms in rat MSCs.Cell viability was determined by3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay.Apoptosis was assessed by Hoechst 33258nuclear staining and flow cytometric analysis with annexin V/PI staining.The gene expression of Toll-like receptor-4(TLR-4)and V-erb-b2 erythroblastic leukemia viral oncogene homolog 2(ErbB2)was detected by real-time polymerase chain reaction(PCR).The protein levels of cleaved caspase-3,Bcl-2,Bcl-xL,Bax,total-ERK,phospho-ERK,totaI-Akt,phospho-Akt,and Hsp90 were detected by Western blot.The production of nitric oxide was measured by spectrophotometric assay.Hsp90 improves MSC viability and protects MSCs against apoptosis induced by serum deprivation and hypoxia.The protective role of Hsp90 not only elevates Bcl-2/Bax and Bcl-xL/Bax expression and attenuates cleaved caspase-3 expression via down-regulating membrane TLR-4 and ErbB2 receptors and then activating their downstream PI3K/Akt and ERK1/2 pathways,but also enhances the paracrine effect of MSCs.These findings demonstrated a novel and effective treatment strategy against MSC apoptosis in cell transplantation.     

利用GSM短消息实现热计量表远程无线抄表系统的设计

针对居民供暖系统采用分户控制、分户计量之后带来的抄表问题,提出利用GSM短消息设计热计量表远程无线抄表系统的方案.给出了系统的总体设计思路以及具体的软硬件设计方案,通过该系统实现了热计量表的远程无线抄表.     

经济新常态下我国体育用品制造企业主要财务指标比较及对策研究

采用文献资料、比较分析、数理统计等研究方法,围绕企业盈利能力、成长能力、运营能力和偿债能力4个方面,对2012~2015年我国不同登记注册类型的体育用品制造企业的主要财务指标进行比较分析。结果显示：（1）体育用品制造企业的整体盈利水平实现正向增长,盈利能力强弱顺序为集体企业、私营企业、股份制企业、外商和港澳台投资企业、股份合作制企业及国有企业;（2）体育用品制造企业增长速度较快,成长能力强弱顺序为国有企业、集体企业、私营企业、股份制企业、外商和港澳台投资企业、股份合作制企业;（3）运营能力强弱顺序为集体企业、股份合作制企业、私营企业、国有企业、外商和港澳台投资企业、股份制企业;（4）企业偿债能力强弱顺序为集体企业、股份合作制企业、国有企业、外商和港澳台投资企业、股份制企业和私营企业;（5）总体而言,近4年来集体所有制体育用品制造企业的各方面能力均较强,但其余5类企业在各方面能力上的强弱差异性过大,两极分化问题非常突出。据此,以新常态下中国经济发展的基本特征为切入点,从企业组织形式转型、创新创业、品牌建设、政策扶持、人才培养、融资约束、贸易环境等8个方面,提出了我国体育用品制造企业发展的对策和建议。     

思想政治教育学科共同体探析

科学共同体作为共同体的一种专业、职业联合体,对于现代学科的发展具有直接借鉴意义。思想政治教育学科共同体在科学共同体的意义上理解和塑造,并依据身份认同、共有理解、共同信念、总体方法论四个基本维度做出进一步的分析,以期摆脱被泛化的尴尬,在基本理论、基本范畴上更深层反映思想政治教育学科的丰富性。     

高校思想政治理论课的价值论

知识教育和信仰教育是思想政治理论课教学中两个重要环节,二者相互促进,不可偏废。知识教育是信仰教育的原生形态,是信仰教育的逻辑表现形式;信仰教育是知识教育的价值取向,是知识教育的最终目的。思想政治理论课教学应该坚持知识教育统一于信仰教育的原则,经由知识教育层面上升到信仰教育层面。     

Integrating ICT into College English: An implementation study of a national reform

This paper describes a mixed-methods case study of a national reform of College English teaching in China which called for the integration of ICT (information and communication technology) into English classroom teaching and self-access learning. The study examined ICT implementation of the national reform in a specific tertiary institution from the perspectives of ICT use in language education and ICT-related policies and practices for EFL (English as a Foreign Language) teachers. The findings indicated that the reform had represented a challenge for teachers, who were expected to adapt to technologically-enhanced teaching materials, embrace student-centred classroom teaching and guide students’ autonomous learning in ICT settings. Constraints included insufficient ICT facilities, teachers’ limited ICT skills and pedagogic expertise; and lack of effective communication networks and inadequate technical support and ICT-related training also hindered the smooth ICT implementation of the reform. Implications for national and institutional policy-making are discussed.     

Comparative features of colorectal and gastric cancers with microsatellite instability in Chinese patients

Objective  

The purpose of this study was to determine the unique and universal features of microsatellite instability-high (MSI-H) colorectal cancer (CRC) and MSI-H gastric cancer (GC) in the Chinese population.     

论体育院校教师的专业素质在教学改革中的重要作用

未来高校体育教育的成败,很大程度上取决于教师的素质,深化高校体育专业的教学改革,以满足社会对合格人才的需要,必须提高教师的专业素质,现就体育教师的专业素质在体育教学改革中所起的作用进行论述。     

Polymer stretch in two-phase microfluidics: Effect of wall wettability

Polymer stretching in two-phase microfluidics is investigated by dissipative particle dynamics. The flow patterns can be controlled by wall wettability, flowrate ratio between two phases, and Reynolds number (Re). For neutral and partially wettable walls, segmented flows are formed and polymer stretching can be controlled by Re and segment length. At high Re, stratified flows are observed and the extension ratio can be tuned by the flowrate ratio. For nonwettable walls, slug flows are formed and polymer stretching can be controlled by Re and slug length. At high Re or flowrate ratio, annular flows are observed and high extension ratio can be easily attained.     

后深蹲振动刺激训练对女子举重运动员后深蹲力量的影响

采用振动台将10名16—18岁女子举重运动员，随机分成实验组Ⅰ与实验组Ⅱ，对两者分别进行后深蹲振动刺激训练与传统后深蹲力量训练的实验观察，结果表明，通过8周训练，实验组Ⅱ比实验组Ⅰ后深蹲力量明显提高。