Evaluation and application of an efficient plant DNA extraction protocol for laboratory and field testing

Nucleic acids in plant tissue lysates can be captured quickly by a cellulose filter paper and prepared for amplification after a quick purification.In this study,a published filter paper strip method was modified by sticking the filter paper on a polyvinyl chloride resin(PVC)sheet.This modified method is named EZ-D,for EASY DNA extraction.Compared with the original cetyl trimethylammonium bromide(CTAB)method,DNA extracted by EZ-D is more efficient in polymerase chain reaction(PCR)amplification due to the more stable performance of the EZ-D stick.The EZ-D method is also faster,easier,and cheaper.PCR analyses showed that DNA extracted from several types of plant tissues by EZ-D was appropriate for specific identification of biological samples.A regular PCR reaction can detect the EZ-D-extracted DNA template at concentration as low as 0.1 ng/μL.Evaluation of the EZ-D showed that DNA extracts could be successfully amplified by PCR reaction for DNA fragments up to 3000 bp in length and up to 80%in GC content.EZ-D was successfully used for DNA extraction from a variety of plant species and plant tissues.Moreover,when EZ-D was combined with the loop-mediated isothermal amplification(LAMP)method,DNA identification of biological samples could be achieved without the need for specialized equipment.As an optimized DNA purification method,EZ-D shows great advantages in application and can be used widely in laboratories where equipment is limited and rapid results are required.     

抖音短视频融入高校社会主义核心价值观教育的路径创新

当前,以抖音平台为代表的短视频平台已经成为网络生活中的重要构成之一,也成为信息传输、网络娱乐、网络社交的重要渠道.作为成长于网络环境的一代,大学生群体对抖音短视频的接触频度相对较高,在其中扮演的角色包括观看者与生产者.由于抖音短视频平台中集散和多元的内容较多,其对高校的社会主义核心价值观产生双重影响.本研究以抖音短视频...     

浅谈校企合作发展继续教育

以当今世界教育改革的大趋势为背景，阐述了校企合作的重要意义，并以理论和实践为基础，从国内外继续教育的发展，分析探讨了校企合作的可行性。     

微波辐射KF/Al_2O_3催化合成肉桂醛

以KF/Al2 O3为碱性催化剂 ,采用微波辐射催化合成肉桂醛 ,通过正交实验和单因素实验确定最佳反应条件。实验结果表明 :乙醛 0 .2mol,苯甲醛 0 .3 0mol,微波辐射功率 1 60W ,辐射时间 1 1min ,产率可达90 .7%。     

参古定 望今制奇－评刘锡庆的写作理论

刘锡庆对现代写作学的理论贡献是多方面的,从基础理论到文体认,纵教学论到史论,都有建树,尤其在基础理论方面,他用力最勤,成就最大,他“望今制奇,参古定法”,把“以文为本”的传统文章学转换为“以写为中心”的现代写作学。     

课程整合——素质教育的推进器——高校政教专业政史类？…

高师课程体系改革要以素质教育为导向,实现课程结构和内容的最优化整合。对政教专业开展政史类三个系列课程的整合安排与教学实践,这种尝试克服了传统的专业教育体制下政教专业政史类课程终结性、封闭性的各种弊端,是实施素质教育的内在要求。课程整合对于实施素质教育起着动力作用,是转变教育观念、推进教育改革的有益尝试。     

车辆工程专业应用型本科生实践能力培养研究

车辆工程专业本科生通过理论学习、专业实验、生产实习和毕业论文等实践环节,使学生具备从事轨道车辆设计、制造、检修、运用等方面的能力。但是,目前重理论,轻应用的现状不符合当下培养应用型本科生的目标。将车辆工程专业本科生的实践能力分为5个一级指标和15个二级指标,利用模糊综合评判方法对其进行综合评价,计算出学生的实践能力综合评价值。在此基础上,找到影响本科生实践能力的主要因素,给出更加合理的改进意见,进一步提高学生的综合实践能力。     

小学数学教学生活化的有效策略

数学是一门与生活紧密相连的学科,俗语有云:“学会数理化,走遍全天下。”其中数学位于首位,足以见得数学知识能够很好地解决实际问题。利用生活化教学策略进行数学教学,能够让小学生对数学内容产生一定的学习兴趣与好奇心,并且能够有效提升学生对数学知识的重视程度,学生愿意主动学习数学,其综合能力才能够有效提升起来。为了实现上述教学效果,本文主要研究小学数学教学生活化的有效策略。     

Is right ventricular mid-septal pacing superior to apical pacing in patients with high degree atrio-ventricular block and moderately depressed left ventricular function?

Objective

We are aimed to investigate whether right ventricular mid-septal pacing (RVMSP) is superior to conventional right ventricular apical pacing (RVAP) in improving clinical functional capacity and left ventricular ejection fraction (LVEF) for patients with high-degree atrio-ventricular block and moderately depressed left ventricle (LV) function.

Methods

Ninety-two patients with high-degree atrio-ventricular block and moderately reduced LVEF (ranging from 35% to 50%) were randomly allocated to RVMSP (n=45) and RVAP (n=47). New York Heart Association (NYHA) functional class, echocardiographic LVEF, and distance during a 6-min walk test (6MWT) were determined at 18 months after pacemaker implantation. Serum levels of N-terminal pro-brain natriuretic peptide (NT-proBNP) were measured using an enzyme-linked immunosorbent assay (ELISA) kit.

Results

Compared with baseline, NYHA functional class remained unchanged at 18 months, distance during 6MWT (485 m vs. 517 m) and LVEF (36.7% vs. 41.8%) were increased, but BNP levels were reduced (2352 pg/ml vs. 710 pg/ml) in the RVMSP group compared with those in the RVAP group, especially in patients with LVEF 35%–40% (for all comparisons, P<0.05). However, clinical function capacity and LV function measurements were not significantly changed in patients with RVAP, despite the pacing measurements being similar in both groups, such as R-wave amplitude and capture threshold.

Conclusions

RVMSP provides a better clinical utility, compared with RVAP, in patients with high-degree atrioventricular block and moderately depressed LV function whose LVEF levels ranged from 35% to 40%.