Tandem Biocatalysis by CotA-TJ102@UIO-66-NH2 and Novozym 435 for Highly Selective Transformation of HMF into FDCA

2,5-Furandicarboxylic acid(FDCA) is a potential biorenewable chemical for applications including plastics, polyamides, drugs, etc. The selective biosynthesis of FDCA from 5-hydroxymethylfurfural(HMF) by a specific enzyme poses a great challenge. In this study, we reported an efficient strategy to produce FDCA from HMF by the tandem biocatalysis of laccase(Cot A-TJ102@UIO-66-NH_2) and Novozym 435. For the first step, a nanoparticle metal–organic framework was synthesized as a carrier to immobilize Cot A-TJ102@UIO-66-NH_2, which was assigned for the production of 5-formyl-2-furancarboxylic acid(FFCA) and featured an enzyme loading of 255.54 mg/g, specific activity of 135.90 U/mg, and solid loading ratio of 99.65%. Under optimal conditions, an ideal FFCA yield of 98.5% was achieved, and the Cot A-TJ102@UIO-66-NH_2 presented a high recycling capacity after 10 cycles. For the second step, Novozym 435 was applied for the further conversion of FFCA into FDCA, presenting a high FDCA yield of 95.5% under the optimized conditions. Novozym 435 also exhibited a high recyclability after eight cycles. As a result, the tandem biocatalysis strategy provided a 94.2% FDCA yield from HMF, indicating its excellence as a method for FDCA production.     

新时期改进党的执政方式的法律思考

中国共产党作为我国的执政党，其执政方式的确立及演变有一个历史过程，取决于各方面的因素。在新的历史条件下，也要适应国情和社会环境的变化而不断的改进，从而使党的领导效能趋向优化，更快的把我们国家带入现代化社会。本文就试图从法律角度来研究我党执政方式的演变与改进，更好的推进依法治国方略的贯彻实施。     

Symmetric alteration of four knots of B-spline and NURBS surfaces

INTRODUCTION Modification of knots of a B-spline curve changes the shape of the curve. In some recent pub-lications (Juhász and Hoffmann, 2001; 2003; Hoff-mann and Juhász, 2004; Li and Wang, 2005), the authors studied the effect of the alteration of a single knot on the shape of the curve. Hoffmann (2004) and Li & Wang (2006) applied these theories into the constrained shape modification of B-spline and NURBS curves. Juhász (2001) and Hoffmann & Ju-hász (2005) studied the effe…     

理智与情感的共融——手机金属材质的隐喻表达

没有感情的设计是空洞的,没有理智的设计是无用的,有情感有理智的设计才是完整的设计。金属材质的手机以理性、功能化为前提,适应了现在追求是科技的理念,又不失给人情感内在、广义的想象空间,顺应了时尚的潮流。将金属作为其材质的手机述说了引起世人共鸣的语言,它是属于一种内在的、广义的语言。情感品质所追求表达的是与人的经验类似的东西,手机的金属材质看似理智的一种设计,但给人一种想象的空间,有着情感化的语言,看似理智与情感的矛盾,却又那么协调的共融。     

对高职高专经贸类专业课程整合的思考

高职经贸类专业必须按照培养目标要求,坚持以就业为导向、以能力为本位的原则进行课程整合,从而使经贸人才的培养与市场需求相吻合.     

日本留学生与政论文学

政论学以日本留学生的创作为世纪先锋，奠定了中国人社科的激进传统。留学生政论学试图构思爱国主义的正面理论，并且释放新民政治的强烈变革感情．提倡积极参与政治管理的人精神，是中国集权体制和官本位化社会所欠缺的。     

冻融、温度等理化因素对卵黄抗体AGP效价的影响

按常规方法制备抗鸡传染性法氏囊炎卵黄抗体（ｙｏｌｋａｎｔｉｂｏｄｙ，ＹＡ），经冻融、温度、机械振荡、光照和防后剂（０．０１％流柳汞、０．０１％叠氮钠和０．５％苯酚）等处理后测定其琼脂扩散试验（Ａｇａｒｇｅｌｎｒｅｄｒｉｔａｔｉｏｎ，ＡＧＰ）效价的变化．实验结果表明，冻融毛次后卵黄抗体的ＡＧＰ效价自１：６４降至１：３２，冻融６次降至１：１６，０．５％苯酚的存在使ＹＡ的ＡＧＰ效价自１：６４降至１：１６；其它物理因素对ＹＡ的ＡＧＰ效价影响不明显．     

Investigation of iodine concentration in salt,water and soil along the coast of Zhejiang,China

Objective: We aim to describe the environment iodine concentration in salt, water and soil along Zhejiang Province coast in the China foreland. It will be helpful for us to judge whether this area is insufficient in iodine and universal iodized salt is necessary or not. Methods: We collected iodized salt samples, drinking water samples (tap water in the towns, and well water or spring water in the villages), water samples from different sources (ditches, lakes, rivers) and soil samples through random sampling in June, 2005. Salt, water and soil iodine was detected by arsenic-cerium redox method. Statistical analysis was expressed as mean±SEMby Windows SPSS 13.0. Results: (1) The iodine concentration in salt was 27.9±4.33 mg/kg (n=108). (2) Seventy-five water samples were collected. The water iodine value was 0.6～84.8 μg/L (mean of 11.66 μg/L). The watershed along the Qiantang River has significantly higher iodine content than the water in Lin'an in mountain area (P＜0.01). The iodine content and mean iodine content of tap water, well or spring water and natural water sources were 4.30±2.43 μg/L (n=34), 23.59±27.74 μg/L (n=19)and 12.72±10.72 μg/L (n=22) respectively. This indicated that among environmental water sources, the ditch iodine content was the highest with river water iodine being the lowest (P＜0.01). (3) Soil iodine value was 0.11～2.93 mg/kg (mean of 1.32 mg/kg).Though there was no statistical difference of soil iodine in different districts (P=0.131), soil iodine content correlated positively with water iodine content. Conclusion: Iodine concentration in salt accords with national policy of adding iodine in salt. Foreland has more iodine in water than mountain area. The data reflected that water and soil iodine in foreland area was not high, which suggests universal iodized salt should be necessary. Environment iodine has relatively close association with pollution.     

论思想政治教育传统思维方式的变革——以“现实的个人”化解思想政治教育思维方式的消极因素

思想政治教育传统的主客二分思维方式把人抽象化、实体化,导致对受教育者真实需求的忽略和教育内容、教育方式的呆板滞后,不能实现个人与社会、政治教育和全面发展教育的协调,从而使思想政治教育改革不能取得令人满意的效果。马克思“现实的个人”思想是对传统实体化抽象人性观的变革,有利于消解与变革思想政治教育旧的思维方式,真正体现出教育的以人为本,确保思想政治教育改革取得更大的实效。     

氯地酚和FSH对猕猴超数排卵的比较及其在兔输卵管内受精的研究

在不考虑月经周期的情况下连续灌喂氯地酚5天,每天50mg,第8天肌注hCG2000IU,对猕猴具有一定的卵泡超数发育作用,最多可使双侧卵巢共24个卵泡发育,最大卵泡直径5—6mm,但不同个体反应变化较大。FSH hCG超排方案(考虑或不考虑月经周期,连续肌注FSH6天,共80—90IU,第8天肌注hCG2500IU)比氯地酚效果较好,最多可使双侧卵巢共28个卵泡发育,卵泡最大直径10mm,另外在1只猕猴观察到5个卵泡破裂将卵排出。电刺激采得之猕猴精子经洗涤后与用以上两种超排方案以卵泡吸得之卵子一同输入兔输卵管进行受精,获得2个原核期受精卵和1个桑椹胚。