Fluid and electrolyte balance in elite male football (soccer) players training in a cool environment

There are few data in the published literature on sweat loss and drinking behaviour in athletes training in a cool environment. Sweat loss and fluid intake were measured in 17 first-team members of an elite soccer team training for 90 min in a cool (5°C, 81% relative humidity) environment. Sweat loss was assessed from the change in body mass after correction for the volume of fluid consumed. Sweat electrolyte content was measured from absorbent patches applied at four skin sites. Mean (?± s) sweat loss during training was 1.69?±?0.45 l (range 1.06?-?2.65 l). Mean fluid intake during training was 423?±?215 ml (44?-?951 ml). There was no apparent relationship between the amount of sweat lost and the volume of fluid consumed during training (r ² = 0.013, P = 0.665). Mean sweat sodium concentration was 42.5?±?13.0 mmol?·?l^?1 and mean sweat potassium concentration was 4.2?±?1.0 mmol?·?l^?1. Total salt (NaCl) loss during training was 4.3?±?1.8 g. The sweat loss data are similar to those recorded in elite players undergoing a similar training session in warm environments, but the volume of fluid ingested is less.     

Curative role of pantothenic acid in brain damage of gamma irradiated rats

Radiation induced brain damage is associated with impairment of mitochondrial functions, variations in the level of neurotransmitters, and oxidative stress. Mitochondrial function is closely linked to the level of neurotransmitters since the precursors are supplied by the Kreb’s cycle intermediates. The objective of this study was to evaluate the influence of pantothenic acid, an essential component in the synthesis of Coenzyme A (CoA), on the activity of the Krebs cycle enzymes, isocitrate dehydrogenase (IDH), α-ketoglutarate dehydrogenase (α-KGDH), and succinate dehydrogenase (SDH); the level of aspartic, glutamic and GABA; the activity of transaminases, and oxidative stress, in the cerebrum of γ-irradiated rats. Pantothenic acid (26 mg/Kg) was orally administered to the rats, 2 h after irradiation and during the following 5 days. Animals were sacrificed the 7th day post-irradiation. The exposure of male albino rats to γ-rays (5 Gy) has triggered oxidative stress notified by a significant elevation in the level of malondialdehyde (MDA), an end product of lipid peroxidation, associated to a significant decrease in the content of phospholipids, and the antioxidant compound glutathione (GSH). The activity of IDH, α-KGDH, and SDH, has significantly decreased, while the level of aspartic, glutamic and GABA has significantly increased. In parallel to these changes, the activity of alanine and aspartate transaminase has significantly increased, compared to their values in the control rats. Pantothenic acid treatment, has significantly attenuated oxidative stress; enhanced the activity of IDH, α-KGDH, and SDH; minimized the increase in the level of amino acids and the activity of transaminases, compared to their values in the cerebrum of irradiated rats. In conclusion, pantothenic acid could improve the level of neurotransmitters amino acids, which depends on the enzymatic activities of Krebs cycle and linked to oxidative stress.     

Microfluidic system for near-patient extraction and detection of miR-122 microRNA biomarker for drug-induced liver injury diagnostics

Drug-induced liver injury (DILI) results in over 100 000 hospital attendances per year in the UK alone and is a leading cause for the post-marketing withdrawal of new drugs, leading to significant financial losses. MicroRNA-122 (miR-122) has been proposed as a sensitive DILI marker although no commercial applications are available yet. Extracellular blood microRNAs (miRNAs) are promising clinical biomarkers but their measurement at point of care remains time-consuming, technically challenging, and expensive. For circulating miRNA to have an impact on healthcare, a key challenge to overcome is the development of rapid and reliable low-cost sample preparation. There is an acknowledged issue with miRNA stability in the presence of hemolysis and platelet activation, and no solution has been demonstrated for fast and robust extraction at the site of blood draw. Here, we report a novel microfluidic platform for the extraction of circulating miR-122 from blood enabled by a vertical approach and gravity-based bubble mixing. The performance of this disposable cartridge was verified by standard quantitative polymerase chain reaction analysis on extracted miR-122. The cartridge performed equivalently or better than standard bench extraction kits. The extraction cartridge was combined with electrochemical impedance spectroscopy to detect miR-122 as an initial proof-of-concept toward an application in point-of-care detection. This platform enables the standardization of sample preparation and the detection of miRNAs at the point of blood draw and in resource limited settings and could aid the introduction of miRNA-based assays into routine clinical practice.     

Participatory strategies,facilitators and community development in Mexico

During the 80s and 90s in Mexico there was a boost in participatory strategies developed, in order to solve serious deficiencies in community development programmes and projects. The mere existence of such methodologies, per se, does not guarantee the conscious and voluntary participation of rural inhabitants. The social energy mobilisation of the rural communities poses a great demand on those who monitor or implement participatory strategies: they have to take into consideration, among others, the preservation of the existing information about the community; the historical and subjective construction of the communities; respect for the existing structure and organisational forms; the specificity of the proposed objectives and their close relationship with specific operational strategies; and, respect for the time and pace of the communities, as well as the time, pace and movement of the organisational processes within these communities.     

Organizational knowledge and collaborative human resource practices as determinants of innovation

A basic premise of the literature states that innovation depends on the ability of firms to manage knowledge. However, despite the major role of services in the economy, studies that assess the role of knowledge in innovation performance are particularly limited in this sector. Moreover, knowledge and human resource practices are intrinsically related concepts, as it is people who generate processes and refine knowledge. An empirical analysis is conducted on the role of organizational knowledge and collaborative human resource practices in innovation performance. The results show that: (i) existing knowledge in a certain domain provides a base for introducing innovations into the domain; (ii) knowledge of a more general nature favours the introduction of various types of innovation; (iii) collaborative human resource practices are an antecedent of product innovation; and (iv) development of organizational knowledge may be based on a broader perspective associated with human resource management.     

The PCC ISNI Pilot: Exploring Identity Management on a Global,Collaborative Scale

Abstract

The Program for Cooperative Cataloging (PCC) conducted a multi-institution Pilot project to contribute metadata to the International Standard Name Identifier (ISNI) database. In addition to describing the goals, structure, and activities undertaken in the Pilot, this article examines some of the experiences of PCC metadata practitioners as they sought to incorporate ISNIs into their metadata workflows and pursue a wide range of ISNI use cases. Lastly, the article outlines next steps that flowed from the Pilot as the PCC has parlayed its newly developing identity management expertise into additional PCC program explorations.     

Feature-positive discriminations during a spatial-search task with humans

During feature-positive operant discriminations, a conditional cue, X, signals whether responses made during a second stimulus, A, are reinforced. Few studies have examined how landmarks, which can be trained to control the spatial distribution of responses during search tasks, might operate under conditional control. We trained college students to search for a target hidden on a computer monitor. Participants learned that responses to a hidden target location signaled by a landmark (e.g., A) would be reinforced only if the landmark was preceded by a colored background display (e.g., X). In Experiment 1, participants received feature-positive training (+←YB/ XA→+/A?/B?) with the hidden target to the right of A and to left of B. Responding during nonreinforced transfer test trials (XB?/YA?) indicated conditional control by the colored background, and spatial accuracy indicated a greater weighting of spatial information provided by the landmark than by the conditional cue. In Experiments 2a and 2b, the location of the target relative to landmark A was conditional on the colored background (+←YA/ XA→+/ ZB→+/ +←C /A?/B?). At test, conditional control and a greater weighting for the landmark’s spatial information were again found, but we also report evidence for spatial interference by the conditional stimulus. Overall, we found that hierarchical accounts best explain the observed differences in response magnitude, whereas spatial accuracy was best explained via spatial learning models that emphasize the reliability, stability, and proximity of landmarks to a target.