冷轧带钢纠偏设备机械故障原因分析

介绍了纠偏设备的工作原理,分析了冷轧厂轧制线上,带钢纠偏设备本体缺陷产生的原因、种类和调整要求。     

澳大利亚职业教育迅速发展的原因探析

20世纪70年代以来，澳大利亚职业教育迅速发展的主要原因是：重视职业教育，建立完整发达的职业教育体系，加大职业教育改革的力度，采取多种措施，加强政府统筹和宏观调控；确立能力本位的职业教育思想，引入竞争机制，建立市场化机制，采取形式多样的灵活的办学体制，不断增加职业教育投资，多渠道筹措职业教育经费，重视职业教育师资队伍建设。     

中国进口非洲石油面临的机遇与挑战

非洲的石油储量巨大，被祢为“第二个海湾地区”。近年来，非洲石油工业发展迅速，成为全球石油勘探活动最活跃的一个新区。在非洲发掘新的市场空间，不仅是西方大国重视非洲的因素，也是中国外交不可忽视的因素。中国进口非洲石油面临着前所未有的机遇，也面临着前所未有的挑战，总的来说机遇大于挑战。     

湖州非物质文化遗产与乡村旅游融合发展研究

非物质文化遗产需要传承和弘扬,地方旅游业发展需要文化支撑,如何将二者融合发展,值得研究。本文收集、整理湖州非物质文化遗产资料,对其数量、类型、分布等进行分析,并实地调查了湖州旅游发展过程中对非物质文化遗产的利用现状,提出湖州乡村旅游应充分利用非物质文化遗产,可从打造舞台盛宴、创新导游解说、打造非遗属性旅游纪念品三个方面提升乡村旅游的文化内涵。这样,既可以更好地保护地方非物质文化遗产,又可以推动地方旅游文化事业,二者的结合是双赢之举。     

Investigation on apoptosis of neuronal cells induced by Amyloid beta-Protein

Objective: To construct a PC12 cell strain with neuronal differentiation, and observe the apoptosis and pro-liferation activity effects induced these cells by Amyloid beta-Protein (Aβ-43). Methods: 1) PC12 cells in logarithmicgrowth phase were subcultured for 24 h. After the culture fluid was changed, the cells were treated with Rat-β-NGF andcultured for 9 days. 2) Neuronal differentiation of PC12 cells in logarithmic growth phase were divided into four groups:control group (0), experimental group (1), experimental group (2) and experimental group (3). The concentrations of Aβ inthe four groups were 0 μmol/L, 1.25 μ mol/L, 2.5 μ mol/L and 5 μmol/L, respectively. The cells were harvested at 24, 48 and72 h later and stained with AnnexinV-FITC/PI after centrifugation and washing. Then flow cytometry was conducted toexamine the apoptosis percentage. 3) NGF-induced PC12 cells were selected and Aβ with different concentrations wasadded. The final concentrations of Aβ were 0 μmol/L, 1.25 μmol/L, 2.5 μmol/L and 5 μ mol/L, respectively. After the cellswere incubated in an atmosphere of 5% CO2 at 37 ℃ in an incubator for 72 h, the OD values were examined. Results: 1)Neuronal differentiated PC 12 cell lines were successfully established. 2) Flow cytometric examination indicated that Aβ(1.25, 2.5, and 5.0 μmol/L) could effectively induce apoptosis of neuronal-differented cells at the 24 h, 48 h and 72 h timepoints. 3) Aβ (0-5.00 μ mol/L) had no obvious effect on proliferation or restraining of the neuronal differentiation of thePC 12 cells after a 72 h interacting process. Conclusion: This investigation revealed successful neuronal differentiation of thePC12 cell strain. The induction of apoptosis of the neurocytes by various concentrations of Aβ was observed and the in-fluence of Aβ on induced proliferation of PC 12 cells by Rat-β-NGF was revealed. This study -05 provide basis for futureresearch on the molecular cure of AD and interdiction of AD evolution.     

谈谈大学生口才训练的必要性和方法

随着社会对人才要求的提高,口才越来越受到重视。对于大学生来说,口头表达能力的好坏,直接影响到他们的择业前景。因此,在校大学生除学好知识技能外,练就一副好口才,既是终生工作的需要,更是寻求就业机会的优势条件。口才训练的方式和途径很多,在校大学生常用的方法主要有朗读、讨论、辩论、演讲等等。     

浅议大学生健美锻炼

早在健美运动诞生之日起,其创始人本·伟德就曾预言:健美将普及到世界的每个角落,最终成为运动之王 健美运动作为能塑造健壮、完美体格,并能增强意志的运动项目而备受青年人的喜爱 掌握正确的锻炼方法及相关的营养、生活规律等方面的知识有助于大学生在锻炼过程中收到事半功倍的效果     

培养学生质疑问难能力策略研究

小学阶段是培养学生质疑问难能力的基础和关键。培养学生质疑问难能力是素质教育的需要，有助于学生良好个性的形成。培养学生质疑问难能力，要研究学生学习心理、学习环境和自身的知识和能力，同时也要教给学生质疑问难的方法。     

高职院校教学管理工作的探索

在新世纪新形势高职院校教学管理工作有新的要求; 目前高职院校在教学管理中存在的问题; 加强高职院校教学管理工作的建议和措施。     

Enhancing cellular immune response to HBV M DNA vaccine in mice by codelivery of interleukin-18 recombinant

Objective: To investigate the effect of interleukin-18 (IL-18) on immune response induced by plasmid encoding hepatitis B virus middle protein antigen and to explore new strategies for prophylactic and therapeutic HBV DNA vaccines. Methods: BALB/c mice were immunized with pCMV-M alone or co-immunized with pcDNA3-18 and pCMV-M and then their sera were collected for analysing anti-HBsAg antibody by ELISA; splenocytes were isolated for detecting specific CTL response and cytokine assay in vitro. Results: The anti-HBs antibody level of mice co-immunized with pcDNA3-18 and pCMV-M was slightly higher than that of mice immunized with pCMV-M alone, but there was not significantly different (P>0.05). Compared with mice injected with pCMV-M, the specific CTL cytotoxity activity of mice immunized with pcDNA3-18 and pCMV-M was significantly enhanced (P<0.05) and the level of IFN-γ in supernatant of splenocytes cultured with HBsAg in vitro was significantly elevated (P<0.05) while the level of IL-4 had no significant difference (P>0.05). Conclusion: The plasmid encoding IL-18 together with HBV M gene DNA vaccines may enhance specific TH1 cells and CTL cellular immune response induced in mice, so that IL-18 is a promising immune adjuvant.