DNA capture-probe based separation of double-stranded polymerase chain reaction amplification products in poly(dimethylsiloxane) microfluidic channels

Herein, we describe the development of a novel primer system that allows for the capture of double-stranded polymerase chain reaction (PCR) amplification products onto a microfluidic channel without any preliminary purification stages. We show that specially designed PCR primers consisting of the main primer sequence and an additional “tag sequence” linked through a poly(ethylene glycol) molecule can be used to generate ds-PCR amplification products tailed with ss-oligonucleotides of two forensically relevant genes (amelogenin and human c-fms (macrophage colony-stimulating factor) proto-oncogene for the CSF-1 receptor (CSF1PO). Furthermore, with a view to enriching and eluting the ds-PCR products of amplification on a capillary electrophoretic-based microfluidic device we describe the capture of the target ds-PCR products onto poly(dimethylsiloxane) microchannels modified with ss-oligonucleotide capture probes.     

Job ads,jobs, and researchers: Searching for valid sources

Research on librarians' roles and responsibilities often takes the form of content analysis of job advertisements found in aggregators such as print journals and websites. Whether these ads help us better understand librarianship depends on how representative the source data is for each study—the line going from jobs, to job ads, then to job ad sources. Print sources dominate even in studies published after 2000. This study examines where reasonably representative job advertisements for academic libraries may be found by starting at the origin: the institutions themselves. It finds that commonly used print sources provide only a small fraction of available positions (the majority of which are those in doctoral institutions), and even the most comprehensive aggregator misses nearly half of the available positions. Taking job ad samples directly from institutions is time-consuming but provides more representative data. Smaller colleges pose a particular challenge for finding ads as few of them have openings at any one time and few of their ads appear in national aggregators.     

为学生营造舒适的学习共享空间——以美国印第安纳州普渡大学印第安纳波利斯分校图书馆为例

对印第安纳州普渡大学印第安纳波利斯分校(IUPUI)图书馆非计算机区和非图书馆区域的系统观察表明,图书馆在个人和小组学习区域选择中有重要作用,图书馆是学生首选的学习场所。研究表明,图书馆座位的设置、空间使用情况包括笔记本电脑的使用需求及性别偏好等都是图书馆设计应该考虑的重要问题。     

Out of the ordinary: recapturing the liberal traditions of a university education through field courses

This article aims to stimulate debate about the nature of field courses in higher education. We use a colloquium format that allows each of us to reflect on the philosophy of field course teaching and our writing endeavours to establish a dialogue between the authors and leave the reader with a sense of curiosity. It starts with a position paper that sets out a particular viewpoint on field courses to which responses were invited. Two of the respondents were experienced field-course teachers in disciplines traditionally associated with this type of curriculum, the other a lecturer in a non-field-course-oriented discipline.     

Being a protégé: an autoethnographic view of three teacher education doctoral programs

Those in professional and academic fields generally perceive mentoring as a positive relationship that enhances the lives of protégés. Kram (1985 Kram, K.E. 1985. Mentoring at work: Developmental relationships in organizational life, Lanham, MD: University Press of America.  [Google Scholar]) posits that such relationships between dyads in organisational life are an evolutionary, developmental process. The purpose of this article is to explore protégés’ perspectives on their mentoring relationships within doctoral programs. In order to lessen the insurmountable distance between writers and readers that is often associated with a traditional research study, we employ an autoethnographic approach to research writing. We share our journey after having studied the mentoring relationships within our own doctoral programs. Qualitative data collected over a two‐year period consist of journal entries, email correspondence, and phone conversations. This research offers insight into the mentor–protégé relationship and processes, and it provides implications for practice for protégés and mentors, as well as future research directions.     

Diversity and Mentoring in the Workplace: A Conversation with Belle Rose Ragins

Given projected increases in workplace diversity, an understanding of diversity’s intersection with mentoring is a critical topic in the literature. This article involved an interview with Belle Rose Ragins, one of the world’s leading thinkers on diversity and mentoring in the workplace. After providing an overview of Ragins’ key achievements and contributions to the mentoring literature, the article delved into the interview, which involves four parts: (a) Ragins’ education and early influences; (b) her views on the extant literature as well as insights around future areas for inquiry; (c) her current research interests; and (d) her views on consulting and teaching.     

The reliability and validity of the pupil behaviour questionnaire: a child classroom behaviour assessment tool

ABSTRACT

This paper assesses the reliability and validity of the teacher-completed Pupil Behaviour Questionnaire (PBQ), by comparing it to the already extensively validated teacher-completed Strengths and Difficulties Questionnaire (SDQ). Participants included 2074 primary school children participating in a universal school-based trial and 41 vulnerable children who were taking part in a study exploring the impact of exclusion from school. Exploratory factor analysis results (first factor accounts for 80.8% of the variation in the items) and the high Cronbach’s alpha value of 0.85 indicate that the PBQ consists of one substantive factor/dimension. Strong correlations between the total PBQ score and the conduct sub-scale (Spearman’s correlation coefficient (r_s) = 0.67) and total difficulties score (r_s = 0.59) of the SDQ indicate convergent validity. This study suggests that the PBQ is a reliable measure, and provides some evidence of validity. Further work is needed to test the PBQ in an older, more diverse populations and to measure sensitivity to change.