Organic Acidurias: An Updated Review

Organic acidurias are an important class of inherited metabolic disorders arising due to defect in intermediary metabolic pathways of carbohydrate, amino acids and fatty acid oxidation. This review summarizes the current knowledge about the important organic acidurias in the Indian population. Specifically, diagnosis and principles of treatment of organic acidurias are covered. The salient features of common organic acidurias as well as their prevalence in various parts of the world are reviewed in some detail.     

Profile of Plasma Amino Acid Levels in Rats Exposed to Acute Hypoxic Hypoxia

The effect of acute hypoxic hypoxia on the profile of plasma amino acids in rats was studied and compared to that resulting from acute liver injury induced by giving carbon tetrachloride. In hypoxic rats exposed to 45% air in N₂ for 5 h, the concentrations of branched chain amino acids, including valine, leucine and isoleucine, and aromatic amino acids such as phenylalanine and tyrosine were significantly increased as compared to those in normoxic rats. The ratio of branched-chain to aromatic amino acids (Fischer’s ratio) was significantly decreased. The levels of arginine and citrulline, which are related to the urea cycle, were also depressed. Furthermore, plasma proline level was reduced in hypoxic rats. The activities of plasma marker enzymes for tissue damage remained unchanged during hypoxia, indicating that tissue injury was not induced by exposure to hypoxic conditions. We suggest that the characteristic profile of plasma amino acids and the Fischer ratio are valuable tools for understanding the pathology of acute hypoxia in the absence of systemic tissue damage.     

Circulating Biomarkers and their Possible Role in Pathogenesis of Chronic Hepatitis B and C Viral Infections

The present study evaluated the plausible role of circulating biomarkers in immune pathogenesis of chronic hepatitis considered a priority in clinical hepatology. Total viral load of chronic hepatitis B virus (HBV) and hepatitis C virus (HCV) patients was quantified and correlation studies were performed with circulating levels of Th1/Th2 cytokines; C reactive protein and circulating nucleosomes; glutathione reductase (GR) and superoxide dismutase. To our knowledge, the study is first among its kind that validates strong positive correlation of viral load with IL-4, IL-6, GR in HBV and IL-6, IL-10, GR in HCV infections. Although, multi-centric studies including large cohorts are required for translating our findings to clinical practice, however, role of these biomarkers with potential diagnostic or prognostic significance might be helpful in clinical assessment of high-risk individuals, thereby, designing interventional strategies, towards development of personalized medicare. The results of our study also offer valuable insights of immune signaling mediators engaged in development of hepatocellular carcinoma.     

Biochemistry of Nitric Oxide

Nitric oxide (NO) a free radical having both cytoprotective as well as tumor promoting agent is formed from l-arginine by converting it to l-citrulline via nitric oxide synthase enzymes. The reaction product of nitric oxide with superoxide generates potent oxidizing agent, peroxynitrite which is the main mediator of tissue and cellular injury. Peroxynitrite is reactive towards many biomolecules which includes amino acids, nucleic acid bases; metal containing compounds, etc. NO metabolites may play a key role in mediating many of the genotoxic/carcinogenic effects as DNA damage, protein or lipid modification, etc. The basic reactions of nitric oxide can be divided as direct effect of the radical where it alone plays a role in either damaging or protecting the cell milieu and an indirect effect in which the byproducts of nitric oxide formed by convergence of two independent radical generating pathways play the role in biological reactions which mainly involve oxidative and nitrosative stress. Nitric oxide is also capable of directly interacting with mitochondria through inhibition of respiration or by permeability transition. Reaction of nitric oxide with metal ions include its direct interaction with the metals or with oxo complexes thereby reducing them to lower valent state. Excessive production of nitric oxide can be studied by inhibiting the synthetic pathway of nitric oxide using both selective or specific nitric oxide synthase inhibitor or non-selective nitric oxide synthase inhibitor with respect to isoforms of nitric oxide.     

A Review on Salivary Genomics and Proteomics Biomarkers in Oral Cancer

Oral cancer has emerged as an alarming public health problem with increasing incidence and mortality rates all over the world. Therefore, the implementation of newer screening and early detection approaches are of utmost importance which could reduce the morbidity and mortality associated with this disease. Sensitive and specific biomarkers for oral cancer are likely to be most effective for screening, diagnosis, staging and follow-up for this dreaded malignancy. Unlike other deep cancers, oral cancer is located in oral cavity. Hence, the direct contact between saliva and oral cancer lesion makes the measurement of tumor markers in saliva an attractive alternative to serum and tissue testing. The DNA, RNA and protein molecules derived from the living cancer cells can be conveniently obtained from saliva. Thus, salivary biomarkers, a non-invasive alternative to serum and tissue-based biomarkers may be an effective modality for early diagnosis, prognostication and monitoring post therapy status. In the current post-genomic era, various technologies provide opportunities for high-throughput approaches to genomics and proteomics; which have been used to evaluate altered expressions of gene and protein targets in saliva of oral cancer patients. The emerging field of salivary biomarkers has great potentials to prove its clinical significance to combat oral cancer. Hence, we have reviewed importance of several salivary genomics and proteomics biomarkers for oral cancer.     

Fear and Loathing in the Fog: The Perceived (and Persistent) Vagaries of Tenure Standards Among Mass Communication Professors

Perhaps nothing strikes more fear into the hearts of all tenure-track professors than the process itself. Are four papers in this journal good enough? Are eight papers in another okay? What if someone doesn’t like me or my area of research? These issues have plagued academia since the first essay was penned by the first researcher hundreds of years ago. While personal bias and journal preference continue to infect honest tenure reviews, solutions exist. This does not mean we have a magic bullet to solve issues of bullying or elitism. However, if we are honest in our desire to improve the Academy, we should avail ourselves of every possible tool for improvement. This essay is based on a survey sent to 2,500 mass communication educators. Of the almost 500 respondents, roughly 10% took the time to express emotions that ranged from fear and confusion to outright rage and anger, all because what ought to be clear is not. The author offers 10 ideas of how academia can improve its evaluation of new faculty, as well as a few suggestions regarding the unnecessary inclusion of personal issues in a process that ought to be about creating better researchers and teachers.     

The Rise of Global Publishing and the Fall of the Dream of the Global Book: The Editing of Peter Carey

The article documents the editorial relationship between Peter Carey and his New York based editor for Knopf, Gary Fisketjon, who worked with Carey on his True History of the Kelly Gang. This account provides the basis for a discussion of how globalised publishing, while promising unity—a single text across all territories—has instead introduced a tension into the previously cohesive triad of author, editor, and the single authorized text. As Fisketjon’s experience lays bare, major contemporary texts that are published in multiple editions in different global centers may well proceed through competing or at least parallel editing processes with different presses, different editors, and in different publishing territories. The authorized single edition, even of major literary texts, has been replaced by competing editions. The single edit and editor have been replaced by competing “servant[s] of the writer” (to use Fisketjon’s phrase). Cohesion, while not quite giving way to disunity, gives way to multiplicity and plurality. The experience of the Kelly Gang book is cast against a longer narrative of Carey’s interactions with editors including the University of Queensland Press (UQP) from the 1970s and Faber from the 1980s.