Oxidative stress was assesed by estimating lipid peroxidation product (LPO) in the form of thiobarbituric acid reactive substances
(TBARS), enzymatic antioxidants in the form of superoxide dismutase (SOD), catalase and nonenzymatic antioxidant vitamins
e.g. vitamin C, β carotene and vitamin E in either serum or plasma or erythrocytes in 190 cases of age related cataract in
the age group of 50–80 years. 190 cases were grouped into three morphological types namely, 73 cases of cortical, 77 cases
of posterior subcapsular and 40 cases of nuclear cataract and values of LPO and antioxidants were compared with 78 cases of
age matched healthy control groups. Plasma TBARS levels were cataract cases when compared with control groups. There were
no significant differences in the erythrocyte levels of catalase and plasma levels of Vit E between cataract cases and control
groups. No significant changes of parameters were seen among three different morphological types of age related cataract.
The present study shows that the oxidative stress may play an important role in the age related cataract. 相似文献
AbstractPurpose: The purpose of this study was to identify and rank the SWOT issues of India's veterinary and animal science education. Design: The data were collected at the Indian Veterinary Research Institute (IVRI) from 168 post-graduate students. The two surveys generated 72% (N=121) and 68% (N=114) response rates, respectively. In the first stage, the vital SWOT issues were listed by respondents and in the second stage, they ranked the top eight issues on a Likert scale. Findings: Uniform curriculum; merit-based admissions; and huge demand and employment prospects were perceived as major strengths. Gaps in curriculum revision and inadequate faculty, colleges and hands-on experience were perceived as key weaknesses. Regular curriculum revisions; novel instructional methods; and single admission tests were important opportunities perceived. Out-dated education model; urban biased admissions; and regional barriers were the major threats perceived. Practical implication: The SWOT issues identified are similar to those faced by veterinary education programmes in many countries with minor variations. Originality/value: The findings are of interest for veterinary faculties in developing and under-developed countries to harmonize their veterinary education systems on par with global norms. The related implications for policy and future directions are discussed. 相似文献
Tuberculosis remains major health problem in India and developing countries Immunodiagnosis has important role in screening,
diagnosis and management of tuberculosis. SEVA TB ES-31 antigen has shown potential in detecting tuberculous IgG antibody
in earlier studies from our laboratory. In the present study we have analysedSEVA TB ES-31 antigen specific immunoglobulinsIgM, IgA and IgG in clinically and bacteriologically confirmed pulmonary tuberculosis cases to determine the usefulness of specific immunoglobulin
class in the diagnosis of patients attending the hospital.
Of the 30 cases of pulmonary tuberculosis 25 (83.3%) were positive for IgG, 19 (63.3%) for IgM and 16 (53.3%) for IgA. On
combining IgG and IgM positivity, sensitivity was increased to 93.3%. While combining IgG and IgA positivity, sensitivity
increased to 90%. However specificity was decreased to 66.6% and 70% for both of these combinations respectively. It could
be envisaged from this study that IgG antibody detection against ES-31 antigen showed acceptable sensitivity (83.3%) and specificity
(86.6%) compared to IgM or IgA alone or in combination. When immune responses were analysed according to degree of sputum
positivity, IgG response was observed to be predominant in all grades, compared to IgM or IgA antibody. The addition of IgM
or IgA as an adjunct test increases the sensitivity but at the cost of specificity. Hence the detection of IgG alone is more
useful compared to IgM or IgA assay, in detecting tuberculosis disease cases coming to the hospital. 相似文献
Lymphatic filariasis caused mainly by infection fromW. bancrofti andB. malayi remains a major cause of clinical morbidity in tropical and subtropical countries. Analysis ofB. malayi mf, infective larval and adult worm lysates for the activity of enzymes led to the demonstration of activities of three key
enzymes of carbohydrate metabolism viz., Malate dehydrogenase (MDH), Malic enzyme (ME) and Glucose-6-phosphate dehydrogenase
(G6PDH) in all the three stages of the parasite. The specific activity of all the three dehydrogenases was significantly high
in mf lysate compared to their activity in lysates of the other two stages (P<0.001). Analysis by native polyacrylamide gel
to their activity inlysates of the other two stages (P<0.001). Analysis by native polyacrylamide gel electrophoresis (PAGE)
using 7.5% non-gradient gel showed the presence of two isoforms of each of the three enzymes (MDH, ME & G6PDH) in mf lysate,
while only one form of each enzyme was present in L3 larval and adult worm lysates. Further proteolytic enzyme activity was demonstrated both in microfilarial and infective larval
lysates ofB. malayi. While both mf and L3 larval lysates showed optimal protease activity at alkaline pH of 9.0, the mf lysate showed increased activity also at pH
3.0. The infective larval lysate was markedly inhibited by Tosylamide-L-Phenylalanine chloromethyl ketone (TPCK), a thiol
protease inhibitor, while the protease activity in mf lysate was significantly inhibited by both TPCK and a serine protease
inhibitor Phenyl Methyl Sulphonyl Flouride (PMSF). In sodium do-decyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE),
using gelatin copolymerized gel, the microfilarial lysate showed 3 protease molecules of 40 kDa, 180 kDa and 200 kDa and the
L3 larval lysate had 6 protease molecules of 18, 25, 37, 49, 70 and 200 kDa size. 相似文献
Sera samples of 7 microscopic haematuria cases collected before and after treatment with Diethylcarbamazine citrate, (DEC),
9 microfilaraemic cases and 19 endemic normal individuals were analysed for filarial antigen and IgG antibody levels. Filarial
antigen was detected in 5 of the 7 microscopic haematuria cases, of which 3 turned negative for antigen after treatment with
DEC. While none of the 7 haematuria cases were positive for filarial IgG antibodies, before the DEC treatment, all of them
turned positive after DEC treatment. The sensitivity and specificity values(to detect mf +ve cases) were 89% and 90% respectively
for the detection of filarial antigen and 78% and 95% respectively for the detection of filarial IgG antibodies. 相似文献
Over the last 40 years, there has been continuous evolution in the design of voice-band modems-starting at a data rate of 300 bits per second in the late 1950s, a rate of 33,600 bits per second has been achieved in 1995. Realising such high data rates over the voice band of 3400 Hz is a remarkable feat, made possible by combining sophisticated techniques from three disciplines, communication theory, signal processing and information theory. In this article, we briefly describe certain advanced ideas, which led to data rates very close to the channel capacity limit, established by Shannon. 相似文献
Purpose: The study aims at finding out relevance and knowledge levels of selected teaching competencies as perceived by educational administrators, faculty and students, in order to assess the training needs of faculty of agricultural universities.
Methodology: Relevance and knowledge levels were tested through a teaching competency questionnaire developed and run on 292 respondents fitting into administrator, faculty and student groups. Needs Assessment Model by Borich [1980. “A Needs Assessment Model for Conducting Follow-up Studies.” Journal of Teacher Education 31 (3): 39–42] is used to identify training needs.
Findings: Results indicate differences in perceptions among groups towards teaching competencies. Prioritized training needs were identified which provide the content and direction for the development of faculty in-service educational programmes.
Practical implications: Faculty of agricultural universities need periodic in-service training programmes in order to improve their teaching competencies so that they become effective and competent teachers in the present educational environment.
Theoretical implications: The statistically validated methodological framework provides for capturing the perception of all stakeholders on the teaching competencies among the faculty members of Agricultural Universities in India, and offers a scope for scaling up the study for similar educational setting in the region.
Originality/value: The perception of students and administrators was also considered along with the self-perception of faculty about the relevance and knowledge levels of teaching competencies. 相似文献
Vitamin B12 is an important coordination compound in biology. It is an interesting biomolecule in the sense that no other vitamin contains a metal ion. This is the only naturally occurring organometallic compound found in biology. An intriguing aspect of vitamin B12 is the great stability of the metal-carbon bond. A great deal of new and interesting inorganic chemistry has been uncovered while studying systems pertinent to B12. In this article some salient features of this unique molecule (B12) and its model compounds are presented. 相似文献
Recent studies have presented evidence for the involvement of L1CAM gene mutations in various X-linked mental retardation
syndromes. The neural cell adhesion molecule, L1CAM is a transmembrane protein belonging to the super family of the immunoglobulins
that play a key role in embryonic development of the nervous system and is involved in memory and learning. No studies were
carried out from India on L1 CAM gene in X-linked mental retardation syndromes. Hence, an investigation was taken up to delineate
the role of L1CAM gene in mental retardation.
Two families (Family I and Family II) having only two members affected with mental retardation in each family were studied
for mutations in L1CAM gene. In family II, the younger sibling showed deletion involving region between the nucleotide 13,773
(intron 25) and 14,158 (intron 27) region. The mutation what we observed in younger sibling of the family II is a novel mutation
which was not hitherto reported in the world literature. 相似文献