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Eric R. Muoz Jaclyn B. Caccese Brittany E. Wilson Kyle T. Shuler Fernando V. Santos Carolina T. Cabn John J. Jeka Dianne Langford Matthew B. Hudson 《运动与健康科学(英文)》2021,10(2):122-130
BackgroundConsidering the potential cumulative effects of repetitive head impact (HI) exposure, we need sensitive biomarkers to track short- and long-term effects. Circulating small extracellular vesicles (sEVs) (<200 nm) traffic biological molecules throughout the body and may have diagnostic value as biomarkers for disease. The purpose of this study was to identify the microRNA (miRNA) profile in circulating sEVs derived from human plasma following repetitive HI exposure.MethodsHealthy adult (aged 18–35 years) soccer players were randomly assigned to one of 3 groups: the HI group performed 10 standing headers, the leg impact group performed 10 soccer ball trapping maneuvers over 10 min, and the control group did not participate in any soccer drills. Plasma was collected before testing and 24 h afterward, and sEVs were isolated and characterized via nanoparticle tracking analysis. Next-generation sequencing was utilized to identify candidate miRNAs isolated from sEVs, and candidate microRNAs were analyzed via quantitative polymerase chain reaction. In silico target prediction was performed using TargetScan (Version 7.0; targetscan.org) and miRWalk (http://mirwalk.umm.uni-heidelberg.de/) programs, and target validation was performed using luciferase reporter vectors with a miR-7844-5p mimic in human embryonic kidney (HEK) 293T/17 cells.ResultsPlasma sEV concentration and size were not affected across time and group following repetitive HI exposure. After 24 h, the HI read count from next-generation sequencing showed a 4-fold or greater increase in miR-92b-5p, miR-423-5p, and miR-24-3p and a 3-fold or greater decrease in miR-7844-5p, miR-144-5p, miR-221-5p, and miR-22-3p. Analysis of quantitative polymerase chain reaction revealed that leg impact did not alter the candidate miRNA levels. To our knowledge, miR-7844-5p is a previously unknown miRNA. We identified 8 miR-7844-5p mRNA targets: protein phosphatase 1 regulatory inhibitor subunit 1B (PPP1R1B), LIM and senescent cell antigen-like domains 1 (LIMS1), autophagy-related 12 (ATG12), microtubule-associated protein 1 light chain 3 beta (MAP1LC3B), integrin subunit alpha-1 (ITGA1), mitogen-activated protein kinase 1 (MAPK1), glycogen synthase kinase 3β (GSK3β), and mitogen-activated protein kinase 8 (MAPK8).ConclusionCollectively, these data indicate repetitive HI exposure alters plasma sEV miRNA content, but not sEV size or number. Furthermore, for the first time we demonstrate that previously unknown miR-7844-5p targets mRNAs known to be involved in mitochondrial apoptosis, autophagy regulation, mood disorders, and neurodegenerative disease. 相似文献
43.
The relationship between peak height velocity and physical performance in youth soccer players 总被引:1,自引:1,他引:0
Philippaerts RM Vaeyens R Janssens M Van Renterghem B Matthys D Craen R Bourgois J Vrijens J Beunen G Malina RM 《Journal of sports sciences》2006,24(3):221-230
Longitudinal changes in height, weight and physical performance were studied in 33 Flemish male youth soccer players from the Ghent Youth Soccer Project. The players' ages at the start of the study ranged from 10.4 to 13.7 years, with a mean age of 12.2 +/- 0.7 years. Longitudinal changes were studied over a 5 year period. Peak height velocity and peak weight velocity were determined using non-smoothed polynomials. The estimations of peak height velocity, peak weight velocity and age at peak height velocity were 9.7 +/- 1.5 cm x year-1, 8.4 +/- 3.0 kg x year-1 and 13.8 +/- 0.8 years, respectively. Peak weight velocity occurred, on average, at the same age as peak height velocity. Balance, speed of limb movement, trunk strength, upper-body muscular endurance, explosive strength, running speed and agility, cardiorespiratory endurance and anaerobic capacity showed peak development at peak height velocity. A plateau in the velocity curves was observed after peak height velocity for upper-body muscular endurance, explosive strength and running speed. Flexibility exhibited peak development during the tear after peak height velocity. Trainers and coaches should be aware of the individual characteristics of the adolescent growth spurt and the training load should also be individualized at this time. 相似文献
44.
Based on ideas from subjective uncertainty reduction theory, we examined the extent to which individuals hold similar sport team preferences to their family and friends. We also assessed the extent to which perceived sport importance and personal need for structure are influential in the development of team identification. Australian high school students aged 15.5+/-1.0 years (mean+/-s) completed questionnaires containing the Personal Need for Structure Scale, the Relational-Interdependent Self-Construal scale, a Team Identification Scale, and items assessing perceptions of sport importance and team preferences. As expected, correlations indicated that preferences for sport teams were significantly related to the preferences of family and friends. Separate analyses of variance confirmed the remaining hypotheses. More specifically, individuals who perceived that sport was important identified with the team more than individuals who perceived sport to be less important. Also, individuals high in personal need for structure scored higher in cognitive/affective team identification than those low in this need. Individuals with differing needs for structure did not differ in their scores on other dimensions of team identification. The results support the notion that cognitive/emotional attachments to sport teams are influenced by perceptions of the importance of sport and by needs for subjective structure. It is possible that other psychological motives, such as the desire for self-esteem, facilitate the development of evaluative dimensions of team identification. 相似文献
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48.
This paper introduces a polynomial operator called the DT-polynomial as a novel approach to network flow problems. The class of networks dealt with is time-varying in the sense that the capacity, cost, and travel-time of each edge may vary in discrete time. The Dt-polynomial is a polynomial in two operators, D (delay) and T (time), which is used for describing the time-varying transmission characteristics. The paper starts with the mathematics involving the DT-polynomials. A new shortest arrival route algorithm is presented, and its computational complexity is found to be favorable in comparison with others such as Dijkstra's method and the potential method derived from Ford-Fulkerson's technique. Furthermore, a dynamic flow problem is formulated and analyzed in terms of DT-polynomials, and a latest-departure earliest-arrival schedule is given. Finally, a modified DT-polynomial is applied to digital filter networks. 相似文献
49.
Robert H. Ennis 《Instructional Science》1974,3(3):285-298
Summary Definitions, I have suggested, have both a function and a form. The function pursued and the form used should depend on the situation and on the term being defined. In the situation described at the outset, Mr. Beta should probably have seen to it that a stipulation of some sort was given-just in order to get on with the task at hand. The stipulation might have been based upon a true reported definition-or it might not-depending on political considerations and the linguistic flexibility of the people concerned. Since no one involved could plausibly have been trying to embody a program in a definition of dough, a programmatic definition was not appropriate in that situation.Several different forms for a definition of dough might reasonably have been used, but in this case the old reliable classification form was probably best, because of its completeness, neatness, and brevity. Two reasonable alternatives are the equivalent-expression form and the range form. The synonym, example-nonexample, and operational forms were probably not appropriate.My main point is that there is not just one way to define. I hope that my delineation of some major possibilities and variations will help those who read this article to be flexible in handling problems of definition when they arise; and they arise more often than most people realize.An ealier draft of this article was presented at a colloquium at the University of Illinois in honor of Professor B. Othanel Smith at his retirement. 相似文献
50.
Walter Hahn Helmut Skowronek H. J. Butcher Hans-Martin Müller-Wolf J. C. Cairns Michael Angermaier Maureen Woodhall Ha Wun Sung Oskar Anweiler John A. Smyth Gilbert de Landsheere Gaby Venstermans Erika Voigt Anza Amen Lema David Wolsk Robert O. Berdahl 《International Review of Education/Internationale Zeitschrift für Erziehungswissenschaft/Revue internationale l'éducation》1974,20(1):83-107