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911.
Jennifer Robertson-Wilson Lucie Lévesque Ronald R. Holden 《Measurement in physical education and exercise science》2013,17(2):93-107
This study was designed to develop the Questionnaire Assessing School Physical Activity Environment (Q--SPACE) based on student perceptions. Twenty-eight items rated on 4-point Likert scales were administered to 244 middle school students in 9 schools. Exploratory factor analysis was used to evaluate the underlying structure of the items and 2 factors were extracted: physical environment (PE) and social environment (SE). Twelve and 8 items loaded saliently on PE (e.g., gym classes available) and SE (e.g., teacher encouragement), respectively. Factor scales had alpha coefficients of .86 (PE) and .81 (SE). One-week test–retest reliabilities for the factor scales of PE and SE were .78 and .72, respectively. Differences in PE scale scores across schools and PE and SE scale scores across student school physical activity behavior (e.g., participation on school teams) provided some evidence of scale construct validity. Overall, Q–SPACE demonstrates acceptable reliability for capturing middle school students' perceptions of school physical activity environment. The factorial validity needs to be assessed with confirmatory factor analysis and invariance testing procedures. 相似文献
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Through a series of structured interviews, university librarians at six institutions provided their perspectives on innovation in academic libraries. The literature on leadership styles and organizational change provides insight into the roles of these leaders in the innovation process. Leadership was cited by many researchers as being a critical factor for organizations to innovate. University librarians revealed a commitment to innovation, some distinctively nontraditional innovations, and a concern for how to encourage risk-taking behavior. Further insight into the innovation process was sought by interpreting the interview data within a larger theoretical context. Although leadership and management can foster innovation in a library, researchers have reported other factors that can influence the ability to innovate, including organizational aspects – size and complexity – and environmental factors. Beyond the organizational aspects, the individual and the norms of the profession appear to create a framework with certain boundaries, some of which may impact the ability to innovate. 相似文献
913.
Vishal Gupta Insiya Jafferji Miguel Garza Vladislava O. Melnikova David K. Hasegawa Ronald Pethig Darren W. Davis 《Biomicrofluidics》2012,6(2)
Isolation and enumeration of circulating tumor cells (CTCs) are used to monitor metastatic disease progression and guide cancer therapy. However, currently available technologies are limited to cells expressing specific cell surface markers, such as epithelial cell adhesion molecule (EpCAM) or have limited specificity because they are based on cell size alone. We developed a device, ApoStream™ that overcomes these limitations by exploiting differences in the biophysical characteristics between cancer cells and normal, healthy blood cells to capture CTCs using dielectrophoretic technology in a microfluidic flow chamber. Further, the system overcomes throughput limitations by operating in continuous mode for efficient isolation and enrichment of CTCs from blood. The performance of the device was optimized using a design of experiment approach for key operating parameters such as frequency, voltage and flow rates, and buffer formulations. Cell spiking studies were conducted using SKOV3 or MDA-MB-231 cell lines that have a high and low expression level of EpCAM, respectively, to demonstrate linearity and precision of recovery independent of EpCAM receptor levels. The average recovery of SKOV3 and MDA-MB-231 cancer cells spiked into approximately 12 × 106 peripheral blood mononuclear cells obtained from 7.5 ml normal human donor blood was 75.4% ± 3.1% (n = 12) and 71.2% ± 1.6% (n = 6), respectively. The intra-day and inter-day precision coefficients of variation of the device were both less than 3%. Linear regression analysis yielded a correlation coefficient (R2) of more than 0.99 for a spiking range of 4–2600 cells. The viability of MDA-MB-231 cancer cells captured with ApoStream was greater than 97.1% and there was no difference in cell growth up to 7 days in culture compared to controls. The ApoStream device demonstrated high precision and linearity of recovery of viable cancer cells independent of their EpCAM expression level. Isolation and enrichment of viable cancer cells from ApoStream enables molecular characterization of CTCs from a wide range of cancer types. 相似文献
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各位专家、各位领导、女士们、先生们:大家上午好!非常高兴,第六届科学计量学与大学评价国际研讨会在武汉大学召开。首先,我代表中国科学院党组副书记、中国科学学与科技政策研究会理事长方新给大会带来良好祝愿!52年前我考入了现在的华中科技大学,从农村 相似文献
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As a relatively new delivery medium, desktop video conferencing (DVC) promises to bring traditional face-to-face teaching to computer screens. Although ample literature covers incorporating technology into classrooms, little documents the experiences and perceptions of participants in DVC courses and DVC's effects on teaching and learning. A recent ethnographic study that examined incidental learning in five DVC courses offered at an American university found that incidental learning often overshadowed the planned curriculum and found a high level of participant frustration. The study's results reaffirmed the importance of incidental learning in a DVC course and of developing an environment that reduces faculty and student frustrations. 相似文献
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