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51.
High-throughput study of alpha-synuclein expression in yeast using microfluidics for control of local cellular microenvironment 总被引:1,自引:0,他引:1
Microfluidics is an emerging technology which allows the miniaturization, integration, and automation of fluid handling processes. Microfluidic systems offer low sample consumption, significantly reduced processing time, and the prospect of massive parallelization. A microfluidic platform was developed for the control of the soluble cellular microenvironment of Saccharomyces cerevisiae cells, which enabled high-throughput monitoring of the controlled expression of alpha-synuclein (aSyn), a protein involved in Parkinson's disease. Y-shaped structures were fabricated using particle desorption mass spectrometry-based soft-lithography techniques to generate biomolecular gradients along a microchannel. Cell traps integrated along the microchannel allowed the positioning and monitoring of cells in precise locations, where different, well-controlled chemical environments were established. S. cerevisiae cells genetically engineered to encode the fusion protein aSyn-GFP (green fluorescent protein) under the control of GAL1, a galactose inducible promoter, were loaded in the microfluidic structure. A galactose concentration gradient was established in the channel and a time-dependent aSyn-GFP expression was obtained as a function of the positioning of cells along the galactose gradient. Our results demonstrate the applicability of this microfluidic platform to the spatiotemporal control of cellular microenvironment and open a range of possibilities for the study of cellular processes based on single-cell analysis. 相似文献
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Dennis Nigbur Rupert Brown Lindsey Cameron Rosa Hossain Anick Landau Dominique Le Touze Adam Rutland Charles Watters 《Int J Intercult Relat》2008,32(6):493-504
Using a sample of white British and British Asian primary-school children (N = 386, aged 5–11 years), we measured acculturation attitudes (own and perceived outgroup), correlated constructs (ingroup and outgroup affect and identification) and relevant outcomes (self-esteem, classroom demeanour) in a structured interview to validate a customised, child-friendly measure of acculturation attitudes based on Berry's framework. Scale items measuring desire for culture maintenance and intergroup contact loaded onto the predicted factors, were internally reliable and showed concurrent validity with affect and identification. The predictive utility of measures was demonstrated in associations between children's acculturation attitudes (or perceived discrepancies with those of the outgroup) and outcomes such as self-esteem and teacher ratings of emotional symptoms. 相似文献
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José Carlos Núñez Natalia Suárez Rebeca Cerezo Julio González-Pienda Pedro Rosário Rosa Mourão 《教育心理学》2015,35(6):726-746
Homework (HW) is one of the most common school-related activities among parents, students and teachers. However, the need to assign HW to students has been extensively debated. The present investigation examines the relationship between specific HW variables (i.e. amount of HW completed, time spent on HW and perceived quality of HW time management) and academic achievement, while controlling for students’ gender and grade level. Participants included 454 students (ranging from 10 to 16?years of age) from three schools in northern Spain. A multivariate analyses of variance and path analyses showed that the amount of HW completed decreased with increased schooling, as did students’ perceived quality of HW time management. Data from hierarchical regression analyses completed by path analyses provided evidence that time spent on HW conjointly with perceived quality of HW time management explains how academic achievement is mediated by the amount of HW completed. These findings deepen the understanding of the complex impact of time spent on HW and on academic achievement. 相似文献
56.
The concept of social presence proposed in studies on communities of inquiry has highlighted both the importance and the challenges of integrating aspects such as affective and relational expressions among team members while solving collaborative learning tasks, in order to advance in a global comprehension of learning processes. This paper presents a theoretical and methodological proposal for tackling this issue from a socio-cultural perspective by analysing the participants’ discourse in relation to expressions of self-competence (at an individual and group level) concerning the task and group cohesion (task cohesion and social cohesion). This proposal’s potential is illustrated by the empirical results of a case study that analyses social presence during a collaborative work task by university students using the Moodle learning platform. 相似文献
57.
In this paper, we analyze the extent to which University-Level Support Mechanisms (ULSMs) and Local-Context Support Mechanisms (LCSMs) complement or substitute for each other in fostering the creation of academic spin-offs. Using a sample of 404 companies spun off from the 64 Italian Science, Technology, Engineering, and Mathematics universities (STEM universities) over the 2000–2007 period, we show that the ULSMs’ marginal effect on universities’ spin-off productivity may be positive or negative depending on the contribution offered by different LCSMs. Specifically, in any given region, ULSMs complement the legislative support offered to high-tech entrepreneurship whereas they have a substitution effect with regard to the amount of regional social capital, regional financial development, the presence of a regional business incubator, regional public R&D expenses as well as the level of innovative performance in the region. Results support the idea that regional settings’ idiosyncrasies should be considered for universities to develop effective spin-off support policies. This paper contributes to the debate on the evaluation of economic policies supporting entrepreneurship. 相似文献
58.
This paper reflects on quality assessment and performance evaluation in higher education, namely by analysing the insufficient
link between those two aspects. We start by reviewing the current state of the art regarding different processes and mechanisms
of quality assessment and performance evaluation and discuss some of the major issues regarding the implementation of some
of them. In particular, we analyse the current limitations regarding data collected, available and publicised on the performance
of HEIs and the problems those limitations bring to a fair evaluation of higher education. Through this analysis we intend
to contribute to a better understanding of the mechanisms of evaluation in higher education and the way these may lead to
the promotion of better quality assessment practices and institutional management. 相似文献
59.
Fernando Rosa 《Inter-Asia Cultural Studies》2013,14(2):214-234
AbstractThis paper attempts to read aspects of language, history, and literariness historically from the perspective of both Brazil and Kerala. It is also an attempt to compare two very distinct places from within a framework centred on the South. Initially, region and nation are discussed, as well as modernity and development, and the issue of thinking South–South connections from an anthropological perspective. Next, the matter of the complex connected histories of Kerala within the Indian Ocean is mentioned, including Portuguese colonial history. The image and influence of Latin America in Kerala is briefly brought up, especially in what concerns literary influence. There follows an extended discussion of issues of literacy and the rise of the novel in Kerala as well as the historical intricacies of the construction of Malayalam as a modern medium. A contrast with the literary construction of modernity in Brazil is made, and the importance of English in Kerala is accordingly highlighted as well as the issue of the coloniality of the Portuguese within Brazil's complex creolized history. An analysis of the pioneering novel of Chandumenon is offered, and a comparison with a novel in Brazil is subsequently sketched. The different historical contexts of nineteenth century Brazil and Kerala are stressed. The close interrelatedness between issues of language, nation, and region is emphasised as well as the internal complexities of modernity in both regions. 相似文献
60.
Leonor Guin Anna Soler Rosa María Lpez Sonia Pajares Jos Manuel Gonzlez de Aledo Ana Argudo-Ramírez Jos Luis Marín Judit García-Villoria ngeles Sahuquillo Luisa Alvarez 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2022,32(3)
IntroductionTo ensure the quality of the new-born screening (NBS), our laboratory reviewed the analytical procedure to detect subjective steps that may represent a risk to the patient. Two subjective activities were identified in the extra-analytical phases: the classification of dried blood spots (DBS) according to their quality and the assignment of haemoglobin patterns. To keep these activities under control, inter-rater studies were implemented. This study aimed to evaluate the inter-rater reliability and the effectiveness of the measures taken to improve the agreement between observers, to assure NBS results’ quality.Materials and methodsDried blood spots specimens were used for the inter-rater studies. Ten studies were performed to assess DBS quality classification, and four to assess the assignment of haemoglobin patterns. Krippendorff’s alpha test was used to estimate inter-rater reliability. Causes were investigated when alpha values were below 0.80.ResultsFor both activities, the reliability obtained in the first studies was inadequate. After investigation, we detected that the criterion to classify a DBS as scant was not consolidated, and also a lack of consensus on whether or not to report Bart’s haemoglobin depending on its percentage. Alpha estimates became higher once the training was reinforced and a consensus about the appropriate criteria to be applied was reached.ConclusionInter-rater reliability assessment helped us to ensure the quality of subjective activities that could add variability to NBS results. Furthermore, the evolution of the alpha value over time allowed us to verify the effectiveness of the measures adopted. 相似文献