Utility of serum indices in a particular case of serum protein electrophoresis

Screening and measurement of monoclonal (M) proteins are commonly performed using capillary zone electrophoresis (CZE). The identification of M-protein or monoclonal component (CM) is an essential requirement for diagnosis and monitoring of monoclonal gammopathies. The detection of CM has been largely improved by CZE. Capillary electrophoresis estimates CM more accurately, because absence of variation due to different dye binding affinities of proteins as instead seen with agarose gel electrophoresis. However, interferences can be present in CZE. This occurs because all substances absorbing at 200 nm can be identified. Recognition and handling of specimens exhibiting such interferences is essential to ensure accurate diagnostic and patient safety. We herein report on an unusual case of serum protein electrophoresis, to highlight that laboratory staff must be aware of and familiarise with the information provided by laboratory instruments. For example, in the case of serum indices, about specimen quality.     

Leucine metabolites do not attenuate training-induced inflammation in young resistance trained men

ABSTRACT

Leucine metabolites may reduce training-induced inflammation; however, there is scant evidence for this assertion. We conducted a double-blind randomized controlled pragmatic trial where 40 male participants were allocated into 4 groups: α-hydroxyisocaproic acid group ([α-HICA], n = 10, Fat-free mass [FFM] = 62.0 ± 7.1 kg), β-hydroxy-β-methylbutyrate free acid group ([HMB-FA], n = 11, FFM = 62.7 ± 10.5 kg), calcium β-hydroxy-β-methylbutyrate group ([HMB-Ca], n = 9, FFM = 65.6 ± 10.1 kg) or placebo group ([PLA]; n = 10, FFM = 64.2 ± 5.7 kg). An 8-week whole-body resistance training routine (3 training sessions per week) was employed to induce gains in skeletal-muscle thickness. Skeletal muscle thickness (MT), one repetition maximum (1RM), interleukin-6 (IL-6), high-sensitivity C-reactive protein (hsCRP) and tumour necrosis factor alpha (TNF-α) were assessed at baseline and at the end of weeks 4 and 8. Time-dependent increases were detected from baseline to week 8 for MT (vastus lateralis: p = 0.009; rectus femoris: p = 0.018), 1RM (back squat: α-HICA, 18.5% ± 18.9%; HMB-FA, 23.2% ± 16%; HMB-Ca, 10.5% ± 13.8%; PLA, 19.7% ± 9% and bench press: α-HICA, 13.8% ± 19.1%; HMB-FA, 15.5% ± 9.3%; HMB-Ca, 10% ± 10.4%; PLA, 14.4 ± 11.3%, both p < 0.001), IL-6, hsCRP (both p < 0.001) and TNF-α (p = 0.045). No differences were found between groups at any time point. No leucine metabolite attenuated inflammation during training. Additionally, backwards elimination regressions showed that no circulating inflammatory marker consistently shared variance with the change in any outcome. Using leucine metabolites to modulate inflammation cannot be recommended from the results obtained herein. Furthermore, increases in inflammatory markers, from training, do not correlate with any outcome variable and are likely the result of training adaptations.     

School to Work： Using Active Learning to Teach Business Writing

To succeed as tomorrow＇s workers in the knowledge society of the new century---a world characterized by ceaseless change, boundless knowledge and endless doubt, today＇s business writing students must develop the skills and traits needed to become creative problem-solvers, flexible team-players and risk-taking life-time learners （Bereiter, 2002a）. And teachers must play an important role in helping students transit successfuUy from school to work by finding ways to develop useful life skills and the flexibility that facilitates a willingness to work cooperatively and a readiness to learn continuously. Preparing today＇s students for tomorrow＇s work world challenges 21st century teachers to reinvent their professional personas by creating a fresh professionalism founded not on old, comfortable abilities and attitudes, but on new, unfamiliar skills and traits. For many teachers （most of whom teach exactly as they were taught--typically, following the talk-chalk model that fills a classroom with five or 10 rows of passive listeners in fixed seats, and fills a class period with 50 or 60 minutes of garbled monotones in lecture format）, learning to teach in ways they were not taught represents the greatest challenge of their careers （Silberman, 1996; Hargreaves, 2003）. Challenging students and teachers to rearrange the furniture and to reconfigure the lecture, a well-considered active learning model （Bonwell ＆ Eison, 1991） can be applied to the business writing classroom （an application unexplored in the literature） to help students develop the abilities and attitudes most required for success in the work world of the 21st century （an area explored in the ＂futuristic business literature＂）--to help students learn and transit. In fact, classes in business writing, professional writing and tecl＇mical writing can function, through the use of active learning strategies, as dynamic workshops in which students can prepare for the change and doubt of the knowledge society by becoming creat     

Internal quality assurance systems in Portugal: what their strengths and weaknesses reveal

In Portugal, the agency for assessment and accreditation of higher education has recently included in its remit, beyond programme accreditation, the certification of internal quality assurance systems. This implies lighter touch accreditation and aims to direct institutions towards improvement, in addition to accountability. Twelve institutions have already undertaken the certification, and both self-assessment and external assessment reports are available. Based on the qualitative analysis of the nature of institutional strengths and weaknesses highlighted in these evaluation reports, the paper aims to understand whether the identified strengths and weaknesses are related to procedural and organisational matters or to cultural change (values and beliefs), in turn offering an insight into the quality culture(s) which characterise higher education institutions in Portugal. Findings suggest that the quality culture of the analysed institutions is somewhere between responsive and reactive. Overall, all reports dwell more on the prioritisation of formal and structural procedures, both regarding strengths and weaknesses. External reports point towards more weaknesses related to stakeholders’ participation. Both aspects are more frequent in polytechnics than in universities. These findings suggest that polytechnics are more reactive, whereas universities are more responsive. Therefore, accountability apparently continues to be, for the time being, a more pressing concern than improvement.     

Academics’ and employers’ perceptions about responsibilities for employability and their initiatives towards its development

This paper reports the results of preliminary research into how Portuguese academics and employers perceive the responsibility of different higher education stakeholders—students, teaching staff, higher education institutions, employers, and policy-makers—for developing graduate employability. The study was conducted 8 years after the implementation of the Bologna Process, the reform that placed employability firmly on the agenda of higher education institutions (HEIs) in Portugal. This paper aims to assess the extent to which higher education is held responsible for developing employability, and to characterize the activities undertaken by the two actors to achieve that end. In particular, with respect to academics, we characterize curricular and other changes to study programmes, and, with respect to employers, their participation in activities undertaken by HEIs meant to ease the transition of students to the labour market. The data comes from a survey responded to by 684 Portuguese academics and 64 employers. Academics and employers alike were found to attribute high responsibility for developing employability to higher education, suggesting that the political message of the Bologna Process regarding the relation between higher education and the labour market has been assimilated. However, the activities reported by both types of respondents indicate only an average commitment to developing employability. Here, the low participation of employers in internal institutional activities is noteworthy, suggesting that the recognition of employers as stakeholders in higher education, as advocated by policy-makers, has yet to happen in Portugal.     

An assessment of a social–cognitive model of academic performance in mathematics in Argentinean middle school students

This study tested a set of hypotheses derived from the model of academic achievement in mathematics of the Social Cognitive Career Theory in a sample of Argentinean middle school students. To this aim, 277 students (male and female; age: 13–15 years) were assessed using the following instruments: logical–mathematical self-efficacy scale, mathematics outcome expectations, mathematics performance goals, and mathematics ability test. All of these instruments had been adapted for use in Argentinean students. Academic achievement in mathematics (i.e., grades obtained on regular school exams) was the variable to be modeled through the path analysis technique. The analysis allowed identification of interrelations among the variables and identification of direct and indirect effects. Academic achievement in mathematics was partially explained by the model. Overall, the results support the theoretical postulates of Social Cognitive Career Theory.     

Digital Publishing in Europe: a Focus on France,Germany, Italy and Spain

In this article we will provide a general overview of the situation of the digital publishing in Europe starting from the insights provided by the Editech conference, the international day of advanced study organized by the Italian Publishers Association and focused on the trends, perspectives and applications of technological innovation in publishing at an international level. It is the main Italian event dedicated to book publishing with the aim of helping publishers define their short- and medium-term digital strategies, while being aware of the opportunities and risks involved in the conversion to digital of their business model. The Editech 2010 main focus had been: the market and the national and international situation of digital publishing, the new buying and reading behaviors of consumers, e-books and content for e-readers and mobile devices, new production processes (XML, ePub, digital printing) and the distribution scenario and the diffusion of digital content.     

Effects of competition level on the centre forward role of men's water polo

This study aimed to compare specific technical and tactical indicators of the team and centre forward role of Euro League, and Italian Serie A1, Serie A2, and Serie B men's water polo club competitions. A notational analysis was performed on 21 water polo matches to evaluate the occurrence of technical and tactical team and centre forward indicators, highlighting differences among championships according to chi-square analyses. Differences emerged for Counterattack (P < 0.001) and Power-Play (P < 0.001) possessions, Even (P < 0.001; Euro League: 3 ± 1, Serie A1: 3 ± 2, Serie A2: 3 ± 2, Serie B: 6 ± 4) and Power-Play (P = 0.001) goals, and exclusions and penalties (P = 0.008) of the team during Even possessions. Relatively to the role analyses, effects emerged for perimeter players playing events (P = 0.049), as well as for centre forwards' goals (P = 0.007) and exclusions and penalties (P < 0.001; Euro League: 8 ± 1, Serie A1: 6 ± 2, Serie A2: 6 ± 2, Serie B: 3 ± 2) occurring at the end of Even possessions. Therefore, in Euro League, and Italian Serie A1 and Serie A2, teams perform a high occurrence of Power-Play possessions following up an exclusion, especially achieved by the centre forward during Even possessions, while, in Italian Serie B, goals were mostly scored during Even possessions, with a relevant contribution from the centre forward role.