Polyelectrolyte multilayer surface functionalization of poly(dimethylsiloxane) (PDMS) for reduction of yeast cell adhesion in microfluidic devices

Polyelectrolyte multilayers (PEMs) based on the combinations poly(diallyldimethylammonium chloride)∕poly(acrylic acid) (PDADMAC∕PAA) and poly(allylamine hydrochloride)∕PAA (PAH∕PAA) were adsorbed on poly(dimethylsiloxane) (PDMS) and tested for nonspecific surface attachment of hydrophobic yeast cells using a parallel plate flow chamber. A custom-made graft copolymer containing poly(ethylene glycol) (PEG) side chains (PAA-g-PEG) was additionally adsorbed on the PEMs as a terminal layer. A suitable PEM modification effectively decreased the adhesion strength of Saccharomyces cerevisiae DSM 2155 to the channel walls. However, a further decrease in initial cell attachment and adhesion strength was observed after adsorption of PAA-g-PEG copolymer onto PEMs from aqueous solution. The results demonstrate that a facile layer-by-layer surface functionalization from aqueous solutions can be successfully applied to reduce cell adhesion strength of S. cerevisiae by at least two orders of magnitude compared to bare PDMS. Therefore, this method is potentially suitable to promote planktonic growth inside capped PDMS-based microfluidic devices if the PEM deposition is completed by a dynamic flow-through process.     

我们都曾是哭鼻子的小屁孩儿

那时花开,流水芳年。 青春很锋利,很容易就划开记忆的容颜,岁月如梭,稍纵即逝,我们还没来得及回忆青涩和苦涩,感受阵痛和欣喜,时间就呼啸而过了。     

虎年说虎

——现役脑残之王:马布里作为当年96黄金一代的球员之一,马布里如今的生活简直可以用穷困潦倒来形容,自从当年他自称天下第一控卫起,马布里的生涯评定便每况愈下。自此,马布里就开始了与全世界对抗的戏码。最初在森林狼,他就因不满加内特拿到亿元高薪而愤然离开,拆散了一对本该是黄金搭档的组合。在转投纽约后,尽管身为纽约之子,但尼克斯宁可掏出两千万也要请这位大爷走人。自从被尼克斯队抛弃之     

终极娱乐——给魔术师和心灵魔术师的导演笔记（节选）

第二章神秘娱乐的层次关系 从我们的这边看出去,我们带给观众的表演是魔术效果（effect）。而从观众的角度来看呢,我们的演出可分成三种不同的感受：     

A multimethod examination of the relationship between coaching behavior and athletes' inherent self-talk

The aim of the present research was to investigate the relationship between coaching behavior and athletes' inherent self-talk (ST). Three studies were conducted. The first study tested the construct validity of the Coaching Behavior Questionnaire (CBQ) in the Greek language, and provided support for its original factor structure. The second study examined the relationships between coaching behavior and athletes' ST in field, with two different samples. The results showed that supportive coaching behavior was positively related to positive ST (in one sample) and negatively related to negative ST (in both samples), whereas negative coaching behavior was negatively related to positive ST (in one sample) and positively related to negative ST (in both samples). Finally, the third study examined the relationships experimentally, to produce evidence regarding the direction of causality. The results showed that variations in coaching behavior affected participants' ST. Overall, the results of the present investigation provided considerable evidence regarding the links between coaching behavior and athletes' ST and suggested that coaches may have an impact on athletes' thoughts.     

Longitudinal examination of the exercise and self-esteem model in middle-aged women

This 2-year prospective study examined the exercise and self-esteem model in middle-aged women (N = 143) previously enrolled in a randomized controlled exercise trial. Across the 2-year period, increases in physical activity (PA) and self-efficacy and reductions in body mass index (BMI) were associated with improved subdomain self-perceptions relative to physical condition, and reductions in BMI were associated with improved subdomain self-perceptions relative to physical condition and body attractiveness. The effects of PA, self-efficacy, and BMI on changes in physical self-worth and global self-esteem were mediated by changes in self-perceptions relative to physical condition and body attractiveness. The results of this longitudinal analysis support the hierarchical and multidimensional structure of self-esteem and indicate that middle-aged women can enhance how they perceive their condition and body attractiveness by continued participation in physical activity, increasing their self-efficacy, and maintaining healthy BMI levels.     

Landing techniques in volleyball

Knee injuries such as anterior cruciate ligament lesions and patellar tendonitis are very frequent in volleyball, and are often attributed to micro traumas that occur during the landing phase of airborne actions. The aim of the present study was to compare different jumping activities during official men's and women's volleyball games. Twelve top-level matches from the Italian men's and women's professional leagues were analysed. The jumps performed during the games were classified according to the landing technique used by the player (left or right foot or both feet together), court position, and ball trajectory. Chi-square analyses were performed to detect differences in landing techniques between the sexes, court positions, and trajectories when serving, attacking, blocking, and setting. Significant differences (P?相似文献   

ATriple Iron triathlon leads to a decrease in total body mass but not to dehydration

A loss in total body mass during an ultraendurance performance is usually attributed to dehydration. We identified the changes in total body mass, fat mass, skeletal muscle mass, and selected markers of hydration status in 31 male nonprofessional ultratriathletes participating in a Triple Iron triathlon involving 11.4 km swimming, 540 km cycling and 126.6 km running. Measurements were taken prior to starting the race and after arrival at the finish line. Total body mass decreased by 1.66 kg (SD = 1.92; -5.3 kg to +1.2 kg; p < .001), skeletal muscle mass by 1.00 kg (SD = 0.90; -2.54 kg to +2.07 kg; p < .001), and fat mass by 0.58 kg (SD = 0.78; -1.74 kg to +0.87 kg; p < .001). The decrease in total body mass was associated with the decrease in skeletal muscle mass (r = .44; p < .05) and fat mass (r = .51; p < .05). Total body water and urinary specific gravity did not significantly change. Plasma urea increased significantly (p < .001); the decrease in skeletal muscle mass and the increase in plasma urea were associated (r = .39; p < .05). We conclude that completing a Triple Iron triathlon leads to decreased total body mass due to reduced fat mass and skeletal muscle mass but not to dehydration. The association of decrease in skeletal muscle mass and increased plasma urea suggests a loss in skeletal muscle mass.     

The influence of exercise intensity on frontal electroencephalographic asymmetry and self-reported affect

The "feel better" effect of exercise has been well established, but the optimal intensity needed to elicit a positive affective response is controversial. In addition, the mechanisms underlying such a response are unclear To clarify these issues, female undergraduate students were monitored for electroencephalographic (EEG) and self-reported affective responses during the recovery period following rest, low, moderate, and high intensities of treadmill running, each lasting 30 min. Frontal EEG asymmetry and self-reported vigor scores following exercise at all three intensities were significantly elevated compared to those observed following rest. The results suggest that steady-state aerobic exercise bouts executed at varying intensities induce a similar affective response during the recovery period when assessed at both the behavioral and psychophysiological levels.