Investigation on apoptosis of neuronal cells induced by Amyloid beta-Protein

Objective: To construct a PC12 cell strain with neuronal differentiation, and observe the apoptosis and pro-liferation activity effects induced these cells by Amyloid beta-Protein (Aβ-43). Methods: 1) PC12 cells in logarithmicgrowth phase were subcultured for 24 h. After the culture fluid was changed, the cells were treated with Rat-β-NGF andcultured for 9 days. 2) Neuronal differentiation of PC12 cells in logarithmic growth phase were divided into four groups:control group (0), experimental group (1), experimental group (2) and experimental group (3). The concentrations of Aβ inthe four groups were 0 μmol/L, 1.25 μ mol/L, 2.5 μ mol/L and 5 μmol/L, respectively. The cells were harvested at 24, 48 and72 h later and stained with AnnexinV-FITC/PI after centrifugation and washing. Then flow cytometry was conducted toexamine the apoptosis percentage. 3) NGF-induced PC12 cells were selected and Aβ with different concentrations wasadded. The final concentrations of Aβ were 0 μmol/L, 1.25 μmol/L, 2.5 μmol/L and 5 μ mol/L, respectively. After the cellswere incubated in an atmosphere of 5% CO2 at 37 ℃ in an incubator for 72 h, the OD values were examined. Results: 1)Neuronal differentiated PC 12 cell lines were successfully established. 2) Flow cytometric examination indicated that Aβ(1.25, 2.5, and 5.0 μmol/L) could effectively induce apoptosis of neuronal-differented cells at the 24 h, 48 h and 72 h timepoints. 3) Aβ (0-5.00 μ mol/L) had no obvious effect on proliferation or restraining of the neuronal differentiation of thePC 12 cells after a 72 h interacting process. Conclusion: This investigation revealed successful neuronal differentiation of thePC12 cell strain. The induction of apoptosis of the neurocytes by various concentrations of Aβ was observed and the in-fluence of Aβ on induced proliferation of PC 12 cells by Rat-β-NGF was revealed. This study -05 provide basis for futureresearch on the molecular cure of AD and interdiction of AD evolution.     

"先锋"辨义和先锋小说

1985年起中国“先锋文学”成为了一种有相对固定指向的命名,实际上评论家对“先锋”的理解并不一致;但八十年代中国先锋小说的勃兴,毕竟显示了一批作家在新时期文学中的不懈探索和实验的可贵努力;而最终的失败,则是暗示了当代作家在吸收、借鉴西方现代文艺思想、技巧时的某些通病。     

略论巩固党的阶级基础与扩大党的群众基础

在新的历史条件下，中国共产党不仅要坚持党的工人阶级先锋队性质，而且要适应知识经济时代的要求，把社会主义现代化建设中出现的代表先进生产力的新的社会阶层和社会群体的优秀分子吸收到党内来，进一步增强党的阶级基础和扩大党的群众基础，始终保持党的先进性。     

高职高专"两课"课堂教学管理模式构建

高职高专“两课”课堂教学管理是整个学校管理的重要组成部分,也是“两课”教学管理工作的重要内容,是学校学生思想政治教育工作的主要平台,是开展学生思想政治教育工作管理的基础和第一级台阶。重视和研究“两课”课堂教学管理工作,对加强和提高“两课”教学的实效性具有十分重要的作用。本文就高职高专“两课”课堂教学管理的定位、基本任务及模式构建提出了一些思考。     

优化环境化学实验教学改革的途径

结合近年来在环境化学实验教学中的经验与体会,考虑以下多方面的改革与创新,如:开展综合性、设计性和研究探索性实验,培养学生的创新意识;将课题组的科研项目引入实验教学,促进教学科研良性循环;改革考核方式;将多媒体教学引入实验教学,开放实验室等。     

Safety of protease inhibitors and Arbidol for SARS-CoV-2 pneumonia in Zhejiang Province,China

The aim of this study was to evaluate the safety of an antiviral regimen of protease inhibitors combined with Arbidol (umifenovir) for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pneumonia patients. The genomic sequence of SARS-CoV-2 is highly homologous to that of SARS-CoV (Zhou et al., 2020). Previously published basic and clinical research on anti-SARS-CoV treatment found that lopinavir/ritonavir (LPV/r) could improve the prognosis of SARS patients (Chan et al., 2003; Chu et al., 2004). Darunavir (DRV) is another protease inhibitor that blocks the binding of SARS-CoV-2 to human angiotensin-converting enzyme 2 (Omotuyi et al., 2020). The broad-spectrum antiviral drug Arbidol (umifenovir) also shows in vitro anti-SARS-CoV activity (Khamitov et al., 2008).     

论高职院校的职业资格证书制度建设

高等职业教育与普通高等教育的专业建设有着很大的区别,高职教育的专业不是普通高等教育意义上的“学科专业,”而主要是“技术专业;”因此,高职院校推行职业资格证书制度也就显得尤为重要。就高职院校的职业资格证书制度建设的意义、发展现状及发展方向,结合国外职业资格制度发展经验,我国高职院校的职业资格证书制度建设势在必行。     

从跨文化角度看《红楼梦》中的习语翻译

汉语习语具有典型的文化特征，其形象处理是否得当，直接影响到翻译的成功与否。(《红楼梦》杨译本的习语翻译从跨文化角度出发，采取了保留形象直译、移植形象意译等不同的翻译策略。     

关于加强电大实践性教学的思考

实践教学是电大培养应用型人才的关键环节,也是电大教育中的一个难点。针对目前实践教学体系不够完善、实践教学师资相对匮乏、实践教学基地不够稳定、实践教学管理不够严密等状况,电大要制定明确的实践教学目标、完善实践教学体系、构建“双师型”教师队伍、加强实践基地建设、改革实践教学的评价方法,以培养社会欢迎的应用型人才。     

教师的人格魅力及其德育价值

传统的德育模式把德育的理解过程等同于智育的认识过程,主要是从外部对学生施加各种影响,德育效果不尽人意.在师生日常相处中所体现的教师人格魅力作为一种潜教育因素对学生的人格体系具有广泛、持久、内在的潜德育价值,比课堂上人生观教育所运用的书面化语言更富有魅力,具有显性的德育课程所不具有的感召力.