Acalculous gallbladder distension in a young child due to HAV infection: Diagnostic dilemma

Gall bladder distension with acute viral acalculous cholecystitis is a rare event in pediatric cases with a high incidence of perforation, gallbladder necrosis and mortality. We report a two and a half year old female child presenting with fever, vomiting, pain abdomen, mild hepatosplenomegaly and tenderness in right hypochondrium. Laboratory investigations revealed hyperbilirubinemia and elevated alkaline phosphatase, but there was no evidence of bacterial or parasitic infection. Serology for viral hepatitis suggested acute Hepatitis A infection. Ultrasonographically, distended inflamed gallbladder without calculous was observed. Finally acute acalculous cholecystitis due to Hepatitis A virus was diagnosed and the child responded to the conservative management.     

Changes in the levels of glycoproteins and glycosaminoglycans in diabetes associated with myocardial infarction

Changes in the levels of glycoprotein (GP) and glycosminoglycans (GAG) were studied in diabetes associated with myocardial infarction in rats. Diabetes was induced by alloxan while myocardial infarction was induced using isoproterenol. Most of the GAG fraction increased in the heart and aorta in diabetes superimposed with myocardial infarction. The carbohydrate of GP also increased in heart while in serum only sialic acid showed an increase. The activity of lysosomal enzymes showed an increase in the case of β-glucuronidase and β-hexosaminidase while in the case of β-galactosidase and β-fucosidase it showed a decrease in the heart.     

Pro-oxidant and anti-oxidant status in patients of sickle cell anaemia

The role of oxidant damage to red cells in sickle cell anaemia has been of interest in recent years. Although, available reports suggest that sickle cell erythrocytes are susceptible to endogenous free radical mediated oxidant damage there remains discrepancy in the status of antioxidant enzymes and antioxidant vitamins in these patients. In view of this, 107 cases of sickle cell anaemia (36 ‘SS’ and 71 ‘AS’ pattern—as confirmed by haemoglobin electrophoresis) were subjected to analysis of malondialdehyde, ascorbic acid, superoxide dismutase and albumin. The results were compared with 54 age and sex matched healthy controls. The results indicate a marked increase in lipid peroxidation and superoxide dismutase levels in both ‘SS’ and ‘AS’ types of sickle cell anaemia as compared to controls. Although no difference was observed in the levels of albumin in these groups the levels of ascorbic acid were significantly depleted in sickle cell anaemia patients. The results are indicative of enhanced lipid peroxidation along with imbalance in the pro-oxidant and antioxidant status in patients of sickle cell anaemia.     

Bibliometrics of global malaria vaccine research

Objectives: This study evaluates malaria vaccine research carried out in different parts of the world during 1972–2004 using different bibliometric indicators. Method: Data have been downloaded from PubMed for the period 1972–2004 using the keywords (malaria* or plasmodium or falciparum) and (vaccine*) in the title and abstract fields. The study examined the pattern of growth of the output, its geographical distribution, profile of different countries in different subfields and pattern of citations using GOOGLE Scholar. Results: Malaria vaccine research output is gradually increasing. The USA, followed by the UK and Australia contributed the highest number of papers. Publication activity has decreased in Switzerland and Sweden, but has increased in Brazil and China. The majority of the countries have focused on the development of asexual blood stage malaria. Citations per paper and incidence of high‐quality papers for the USA, the UK, Papua New Guinea and Denmark are more than the average. The majority of the prolific institutions are located in the USA, the UK, France and Australia. Conclusion: The last two decades have witnessed considerable growth in research output in this field, while a successful malaria vaccine still remains elusive. Interestingly, the countries like the USA, the UK and Australia that lead in the quantity, quality and citation of this output are often not those directly affected by malaria.     

A sensitive assay for ornithine amino transferase in rat brain mitochondria by ninhydrin method

To establish/develop an assay method for measuring Ornithine Aminotransferase (EC.2.6.1.13) activity using rat brain mitochondria as a source of enzyme in presence and absence of Pyridoxal Phosphate (PLP). The modified method, with the improved sensitivity, is adopted for the assay of ornithine amino transferase activity in rat brain mitochondria. The enzyme activity was measured at 620 nm, the study showed that reaction was optimum at 37°C for 30 minutes. The assay is sensitive enough to detect activity at the order of nanomoles pyrroline-5-carboxylate/mg protein/minute and can be compared as an alternative to the radio isotopic method which is more cumbersome and aminobenzaldehyde method which is less sensitive. The K_m & V_max shows maximum activity in the presence of Pyridoxal Phosphate (Coenzyme) concentration at 0.05mM when compared with absence of Pyridoxal Phosphate as higher the concentration of Pyridoxal Phosphate affects the affinity of the enzyme to substrate. The OAT activity in different tissues of the rat was also studied and highest activity was found in liver and kidney.     

Lipid peroxidation and antioxidant status in blood of rheumatoid arthritis patients

The main objective of the study was to assess the oxidative stress in plasma and erythrocytes of rheumatoid arthritis patients by measuring the levels of thiobarbituric acid reactive substances (TBARS), non-enzymatic antioxidants (vitamin E, C and reduced glutathione) and enzymatic antioxidants [superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSHPx)]. This study has been conducted on twenty-two adult female rheumatoid arthritis patients and an equal number of healthy subjects. Elevated lipid peroxidation and multidirectional changes in the antioxidant defence system were noticed in patients with rheumatoid arthritis. The enhanced lipid peroxidation accompanied by disturbance in antioxidant status indicates that rheumatoid arthritis patients are more prone to free radical mediated oxidative damage.     

Biotin metabolism defect - A case report

Defects in biotin metabolism are mainly associated with either the enzyme Biotinidase or Holocarboxylase synthetase. Defects in either enzymes depletes biotin utilization by the cells. Holocarboxylase synthetase deficiency is an inherited disorder in which the body is unable to use the vitamin biotin effectively. This condition is inherited in an autosomal recessive pattern. We present a case of a 9 year old girl with atypical symptomology as a case holocarboxylase synthetase deficiency, who demonstrated an increased excretion of propionic and methyl malonic acids, with her biotinidase activity being normal. She demonstrated remarkable improvement on biotin supplementation.     

Sex hormone binding globulin in breast cancer

The present study was undertaken to determine the significance of sex hormone binding globulin, the major and specific binding protein for testosterone and estradiol, in breast cancer. Among breast cancer patients, lower serum levels of Sex hormone binding globulin and higher levels of testosterone were observed. Sex hormone binding globulin showed an inverse relationship with testosterone and total cholesterol, and a direct relation with HDL-cholesterol. By the western blot analyses, Sex hormone binding globulin was detected in all biological samples that we examined. In the breast tumor tissue sections, immuno-staining for Sex hormone binding globulin was confined in cell cytoplasm and 29% cases were positive, which showed no association with the investigated prognostic markers of breast cancer such as ER and HER-2/neu over-expression. In this study, decreased circulating levels of Sex hormone binding globulin in breast cancer patients possibly indicate higher bioavailable estrogens.