Improved Breast Cancer Classification Through Combining Graph Convolutional Network and Convolutional Neural Network

AimIn a pilot study to improve detection of malignant lesions in breast mammograms, we aimed to develop a new method called BDR-CNN-GCN, combining two advanced neural networks: (i) graph convolutional network (GCN); and (ii) convolutional neural network (CNN).MethodWe utilised a standard 8-layer CNN, then integrated two improvement techniques: (i) batch normalization (BN) and (ii) dropout (DO). Finally, we utilized rank-based stochastic pooling (RSP) to substitute the traditional max pooling. This resulted in BDR-CNN, which is a combination of CNN, BN, DO, and RSP. This BDR-CNN was hybridized with a two-layer GCN, and yielded our BDR-CNN-GCN model which was then utilized for analysis of breast mammograms as a 14-way data augmentation method.ResultsAs proof of concept, we ran our BDR-CNN-GCN algorithm 10 times on the breast mini-MIAS dataset (containing 322 mammographic images), achieving a sensitivity of 96.20±2.90%, a specificity of 96.00±2.31% and an accuracy of 96.10±1.60%.ConclusionOur BDR-CNN-GCN showed improved performance compared to five proposed neural network models and 15 state-of-the-art breast cancer detection approaches, proving to be an effective method for data augmentation and improved detection of malignant breast masses.     

Synthesis and anticonvulsant activity of some potent 5,6-bis aryl 1,2,4-triazines

In the present research, a series of 5,6-bis aryl 1,2,4-triazines 5a~5f were synthesized by condensation of various benzils 4a~4f with aminoguanidine bicarbonate and were screened in vivo, for their anticonvulsant and neurotoxicity studies. Compounds 5a, 5b and 5d were found to be potent molecules of this series, when compared with the reference drugs phenytoin sodium, diazepam and lamotrigine. The structures of these compounds were established by IR, 1H NMR, 13C NMR and mass spectroscopic data.     

Biotin metabolism defect - A case report

Defects in biotin metabolism are mainly associated with either the enzyme Biotinidase or Holocarboxylase synthetase. Defects in either enzymes depletes biotin utilization by the cells. Holocarboxylase synthetase deficiency is an inherited disorder in which the body is unable to use the vitamin biotin effectively. This condition is inherited in an autosomal recessive pattern. We present a case of a 9 year old girl with atypical symptomology as a case holocarboxylase synthetase deficiency, who demonstrated an increased excretion of propionic and methyl malonic acids, with her biotinidase activity being normal. She demonstrated remarkable improvement on biotin supplementation.     

Beyond Native and Nonnative: Translingual Dispositions for More Inclusive Teacher Identity in Language and Literacy Education

ABSTRACT

This article proposes translingual dispositions as a way to move beyond the NES/NNES dichotomy in understanding language teacher identity. Recent scholarship in TESOL and Applied Linguistics has problematized the NES/NNES binary from a poststructuralist perspective, highlighting how NES/NNES subjectivities are discursively and performatively (co-)constructed and negotiated. Despite the efforts to empower NNESs, the very binary reifies monolingual ideologies. This article argues that translingual dispositions can help move beyond the binary and complicate theorization of teachers’ identities and practice. To illustrate, we report an ethnographic case study of a “NES” teacher, Daphne, and examine how Daphne’s translingual dispositions shape her teaching and facilitate her students’ creative negotiation of monolingual norms in a writing course. We conclude by discussing future research directions and the implications for language teacher education in fostering translingual dispositions.     

One hundred years of peptide chemistry

Emil Fischer is considered to be the founding father of the field of peptide chemistry and originator of the term peptide. In the beginning of the 20th century, Fischer had a clear vision to foresee the day when a protein would be synthesized. Subsequent to this brilliant beginning, progress however was slow for the next 50 years. In 1953, the chemical synthesis of oxytocin by duVigneaud was a landmark achievement. The subsequent decades witnessed the discovery of a vast number of biologically active peptides. The last three decades saw development of multiple simultaneous syntheses, peptide libraries and peptide vaccines. This article is a concise account of the types of peptides discovered and their biological role.     

Microfluidics platform for measurement of volume changes in immobilized intestinal enteroids

Intestinal enteroids are ex vivo primary cultured single-layer epithelial cell spheroids of average diameter ∼150 μm with luminal surface facing inward. Measurement of enteroid swelling in response to secretagogues has been applied to genetic testing in cystic fibrosis and evaluation of drug candidates for cystic fibrosis and secretory diarrheas. The current measurement method involves manual addition of drugs and solutions to enteroids embedded in a Matrigel matrix and estimation of volume changes from confocal images of fluorescently stained enteroids. We developed a microfluidics platform for efficient trapping and immobilization of enteroids for quantitative measurement of volume changes. Multiple enteroids are trapped in a “pinball machine-like” array of polydimethylsiloxane posts for measurement of volume changes in unlabeled enteroids by imaging of an extracellular, high-molecular weight fluorescent dye. Measurement accuracy was validated using slowly expanding air bubbles. The method was applied to measure swelling of mouse jejunal enteroids in response to an osmotic challenge and cholera toxin-induced chloride secretion. The microfluidics platform allows for parallel measurement of volume changes on multiple enteroids during continuous superfusion, without an immobilizing matrix, and for quantitative volume determination without chemical labeling or assumptions about enteroid shape changes during swelling.