The filament forming reactions of vimentin tetramers studied in a serial-inlet microflow device by small angle x-ray scattering

The structural organization of metazoan cells and their shape are established through the coordinated interaction of a composite network consisting of three individual filament systems, collectively termed the cytoskeleton. Specifically, microtubules and actin filaments, which assemble from monomeric globular proteins, provide polar structures that serve motor proteins as tracks. In contrast, intermediate filaments (IFs) assemble from highly charged, extended coiled coils in a hierarchical assembly mechanism of lateral and longitudinal interaction steps into non-polar structures. IF proteins are expressed in a distinctly tissue-specific way and thereby serve to generate the precise plasticity of the respective cells and tissues. Accordingly, in the cell, numerous parameters such as pH and salt concentration are adjusted such that the generation of functional networks is ensured. Here, we transfer the problem for the mesenchymal IF protein vimentin to an in vitro setting and combine small angle x-ray scattering with microfluidics and finite element method simulations. Our approach is adapted to resolve the early assembly steps, which take place in the sub-second to second range. In particular, we reveal the influence of ion species and concentrations on the assembly. By tuning the flow rates and thus concentration profiles, we find a minimal critical salt concentration for the initiation of the assembly. Furthermore, our analysis of the surface sensitive Porod regime in the x-ray data reveals that the formation of first assembly intermediates, so-called unit length filaments, is not a one-step reaction but consists of distinct consecutive lateral association steps followed by radial compaction as well as smoothening of the surface of the full-width filament.     

A Comparison of Phonological Awareness,Lexical Compounding,and Homophone Training for Chinese Word Reading in Hong Kong Kindergartners

Research Findings: In this study, 88 kindergartners received special training in lexical compounding, homophone awareness, or phonological awareness or were assigned to a control condition over a period of approximately 2 months, with 20-min lessons administered twice per week. Chinese word reading improved significantly more in the lexical compounding group as compared to the other groups. Vocabulary knowledge also showed a trend toward improvement in this group (p < .08) and improved significantly in the homophone group. Although phonological awareness improved most in the phonological awareness training group, this group showed no reading or vocabulary improvements relative to the other groups. Practice or Policy: The results underscore the importance of morphological awareness training for both word reading and vocabulary knowledge in young Chinese children.     

Time to embrace the complexity when analysing GPS data? A systematic review of contextual factors on match running in rugby league

ABSTRACT

This systematic review aimed to identify and summarise associations between currently identified contextual factors and match running in senior male professional rugby league. Eligible articles included at least one contextual factor and used GPS to measure at least one displacement variable within competitive senior, male, professional rugby league matches. Of the 15 included studies, the identified contextual factors were grouped into factors related to individual characteristics (n = 3), match result (n = 4), team strength (n = 2), opposition strength (n = 3), match conditions (n = 6), technical and tactical demands (n = 6), spatial and temporal characteristics (n = 7), and nutrition (n = 1). Speed was the most commonly reported measure of match running (100%), followed by distance (47%), and acceleration (20%). Inconsistencies were found between studies for most contextual factors on match running. Higher speeds were generally associated with higher fitness, encountered earlier in the match and whilst defending. All 15 studies utilised a univariate approach to quantify associations of a contextual factor. The inconsistencies found in the associations of given contextual factors highlight the complex and multi-faceted nature of match running. Therefore, practitioners should consider contextual factors when analysing and interpreting GPS data.     

Visual cliff performance in 10 species of muroid rodents

A total of 200 muroid rodents, 20 from each of 10 species from diverse ecological adaptations, were tested for depth perception near the age of weaning. Each animal was given 10 trials in a visual cliff. Seven of the 10 species, Israeli gerbils, California voles, house mice, meadow voles, cactus mice, cotton mice, and deer mice, showed some evidence of depth discrimination. By contrast, white-footed mice, prairie voles, and montane voles showed no evidence of such discriminative ability. Although there was no strong relationship apparent between the usual habitat of the species and their tendency to choose the shallow side in the visual cliff, a relationship was found among ecological factors, open-field behavior, and the latency to make a descent from the centerboard of the visual cliff.     

Uplift,climate and biotic changes at the Eocene–Oligocene transition in south-eastern Tibet

The uplift history of south-eastern Tibet is crucial to understanding processes driving the tectonic evolution of the Tibetan Plateau and surrounding areas. Underpinning existing palaeoaltimetric studies has been regional mapping based in large part on biostratigraphy that assumes a Neogene modernization of the highly diverse, but threatened, Asian biota. Here, with new radiometric dating and newly collected plant-fossil archives, we quantify the surface height of part of the south-eastern margin of Tibet in the latest Eocene (∼34 Ma) to be ∼3 km and rising, possibly attaining its present elevation (3.9 km) in the early Oligocene. We also find that the Eocene–Oligocene transition in south-eastern Tibet witnessed leaf-size diminution and a floral composition change from sub-tropical/warm temperate to cool temperate, likely reflective of both uplift and secular climate change, and that, by the latest Eocene, floral modernization on Tibet had already taken place, implying modernization was deeply rooted in the Palaeogene.     

Being more human: rooting learning analytics through resistance and reconnection with the values of higher education

ABSTRACT

Universities are now compelled to attend to metrics that (re)shape our conceptualisation of the student experience. New technologies such as learning analytics (LA) promise the ability to target personalised support to profiled ‘at risk’ students through mapping large-scale historic student engagement data such as attendance, library use, and virtual learning environment activity as well as demographic information and typical student outcomes. Yet serious ethical and implementation issues remain. Data-driven labelling of students as ‘high risk’, ‘hard to reach’ or ‘vulnerable’ creates conflict between promoting personal growth and human flourishing and treating people merely as data points. This article argues that universities must resist the assumption that numbers and algorithms alone can solve the ‘problem’ of student retention and performance; rather, LA work must be underpinned by a reconnection with the agreed values relating to the purpose of higher education, including democratic engagement, recognition of diverse and individual experience, and processes of becoming. Such a reconnection, this article contends, is possible when LA work is designed and implemented in genuine collaboration and partnership with students.     

#MeToo as networked collective: examining consciousness-raising on wild public networks

ABSTRACT

In 2017, the #MeToo movement swept across social media, making the pervasiveness of sexual assault visible to millions. People shared hashtags and stories often only told in intimate settings on a variety of platforms, exposing this issue to the multitudes. At its core, the #MeToo movement was a consciousness-raising effort, but it was occurring in a new mediascape that dramatically altered its spread, force, and impact. This paper examines #MeToo as it unfolded across wild public networks and, in doing so, resisted contemporary image practices, transformed the rhetor into what we term a rhizomatically networked collective, and expanded audiences.     

4Ps: A purposeful introduction

The 4Ps activity provides a unique approach to first-day class introductions and creates a positive classroom climate that encourages student engagement. The assignment generates initial self-disclosure that facilitates interpersonal and group communication throughout the semester. Additionally, the activity can be used as a unit activity with a follow-up assignment that introduces foundational public speaking concepts.

Courses: Public Speaking, Interpersonal, and other communication courses.

Objectives:

• To promote connectedness within the class from the first meeting forward

• To extend the usual “icebreaker” in a meaningful way by incorporating a public-speaking assignment

• To provide a basis for discussion of key communication concepts such as self-disclosure, relational development, and stereotyping.

  Materials: 4×6 note cards

     

Enhancing Alphabet Knowledge Instruction: Research Implications and Practical Strategies for Early Childhood Educators

Alphabet knowledge is consistently recognized as the strongest, most durable predictor of later literacy achievement. Recent research offers practical implications for increased effectiveness of teaching alphabet knowledge to young children. In this article, we outline Enhanced Alphabet Knowledge instruction (EAK), a method of practical instruction that early childhood teachers can use to organize, plan, and teach the essential skills of alphabet knowledge. EAK emphasizes identifying the letter name and sound, recognizing the letter in text, and producing the letter form, through flexible, distributed cycles of review based on factors that influence acquisition of alphabet knowledge.