用于表达多肽抗原融合基因的杆状病毒系统

以含翻译起始密码子ATG的质粒pSⅪⅤⅥ Ⅹ3/4为转移载体,将多角体蛋白及切除部分5′和3′端的乙型肝炎核心抗原(HBcAg)基因同时插入粉纹夜蛾核型多角体病毒(TnNPV)基因组中,构建了形成多角体的重组毒株。该毒株能利用合成—多角体ⅪⅤ串联启动子,表达由截短的HBcAg序列及其上下游多接头与杆状病毒DNA部分序列组成的融合蛋白基因。SDS-聚丙烯酰胺凝胶电泳、Wcstern印迹与免疫电镜观察的结果表明,融合蛋白基因表达产物的分子量约20.5KD,与理论计算值相符,并保留了HBcAg的抗原性,亦能形成典型的HBcAg颗粒。组建含多克隆点及截去两端序列的HBcAg基因转移载体质粒,使编码多肽抗原的寡聚核苷酸易于克隆,以HBcAg融合蛋白方式在杆状病毒载体系统中表达,并能形成HBcAg为蛋白载体的抗原颗粒结构。     

“学会共存”的教育理想与实践——“德洛尔报告”的重新解读

1996年,国际21世纪教育委员会向联合国教科文组织提交了“德洛尔报告”,即《教育———财富蕴藏其中》。该报告提出的建立终身教育体系的4个支柱已成为目前国际教育改革的共同目标,“学会共存”是这四个支柱的核心。十年来,人们以不同的理念、不同的方式建构和实践着“学会共存”的教育理想,提出了和平文化教育、国际理解教育、世界公民教育等一系列的教育理念和行动策略。“学会共存”教育的基础是人类普适伦理或共享价值,“学会共存”教育的过程是在文化互动中人们对普遍文化价值的建构过程。“学会共存”教育对于展现一个和平、负责任的国家形象,培养具有开放的世界意识和跨文化适应能力的未来公民,具有极为重要的意义。     

自然条件下柑桔潜叶蛾发育历期的研究

研究了自然条件下温度因子对柑桔潜叶蛾发育历期的影响,建立了发育历期与温度相互关系的数学模型,并分别求出了柑桔潜叶蛾卵期、幼虫期、预蛹期和蛹期的田间最适发育平均温度分别为26.42℃,26.84℃,26.45℃,27.69℃,相应最短发育历期分别为1.94±0.394d,4.99±0.44d,0.98±0.012d,5.20±0.28d;柑桔潜叶蛾的最适发育温区为(26.4℃,27.7℃)。     

A co-evolutionary model for supporting the emergence of authenticity

The purpose of this study was to share our experiences using emerging technologies to create an authentic learning context where preservice teachers at a university and practicing K-12 teachers collaborate in the conduct of real-world (as opposed to textbook) tasks. In this paper, we demonstrate and evaluate the design of professional development that involved a partnership between two universities and eight surrounding K-12 schools. This partnership provides the foundation for supporting a learning community of preservice and practicing teachers that situates in collaborative practices that are both authentic and valuable to all involved. Specifically, we studied how issues of ownership, power, authenticity, and collaboration contribute to students' successes and the success of the program through four case studies. We also explored how asynchronous conferencing tools might be used to facilitate communication across geographic and chronological boundaries, breaking down traditional barriers to distributed communities of practice and making possible the creation of a co-evolutionary model for supporting the emergence of a context that was authentic to both preservice and in-service teachers. In contrast to claims that suggest authenticity for an individual can be prescribed to a learner by the instructor, we deny the legitimacy of preauthentication. Instead, an assumption underlying this research is that authenticity is an emergent process that is actualized through individuals' participation in tasks and practices of value to themselves and to a community of practice. The co-evolutionary model for supporting the emergence of authenticity described in this study provides a means of overcoming some of the challenges associated with simulation and participation models for establishing authentic learning experiences.     

Parameters optimization for exponentially weighted moving average control chart using generalized regression neural network

1 Introduction a The statistical process monitoring and quality control in the industry manufacturing using exponentially weighted moving average (EWMA) control chart have attracted much attention recently. Parameter selection is the key to optimal design…     

CAD/CAE system for the ring planetary reducer with small tooth number difference

A CAD／CAE system of the ring-type planetary reducer with small tooth number difference is presented.It is a parameterized CAD/CAE system adopting the object-oriented technique and comprising in itself the geometric feature database(including the modules of ring-plates with ineer te4eth,eccentric shaft modules and output shaft modules),standard componentdatabase and material database.In cdomparison with design by handwork,this computer aided design and analysis system has the advantages of improved quality,shortened design period and reduced cost.The reliability of the system has been verified by a illustrative example.     

应用昆虫体生产乳糖酶的纯化方法及理化性质的研究

本文将已构建好的带有乳糖酶基因的杆状病毒(AcNPV p10 Z-9)作为载体,经口服感染人工饲养的银纹夜蛾(Argyrogra(?) agnata)幼虫,在昆虫体内高效表达乳糖酶蛋白,表达水平可占虫体可溶性蛋白的20％。将虫匀浆后,经高速离心、丙酮沉淀及冷冻干燥等方法,即可制备成粗酶。进一步经FPLC β-aminophenyl-β-D-thiogalactopyranoside亲和柱层析方法制备成精制酶,可作为试剂及药物应用.经测定纯化后的酶比活为150000u/mg蛋白。酶活力的最适pH值是6.0～8.0,最适温度为30～40℃。该酶水解对硝基苯-β-D-半乳糖(ONPG)的米氏常数(Km)值为7.14×10~(4)mol/L,乳糖可竞争性地抑制乳糖酶水解ONPG的能力,Ki值为1.30×10~(-3)mol/L。     

Numerical simulations on rock damage and fragmentation by blasting

1. Introduction In order to study rock damage and fragmentation during blasting, many numerical models have been developed. These efforts have varied from semi-empirical [1,2] to formulations based on shock wave physics [3,4]. Numerical modeling of blasting has been focusing on the complexity of the blasting process. This complexity requires that numerical models be developed in a highly systematic fashion in close coordination with controlled field and laboratory experimentation. This work d…     

Random torsional vibration in automobile transmissions

1. Introduction Transmissions which consist of many different and complicate components as clutches, gearboxes, transversal shafts, etc., are a very important part of automobiles. The torsional vibrations in transmissions affect directly their performances. Many researchers have studied the free and periodic torsional vibrations, the noise and the nonlinear in automobile transmissions [1, 2] etc., considering only the excitation of engine. But the random torsional vibrations excited by road pr…     

Applications of airtight concrete grouting to road tunnels

Road tunnel excavation often traverses coal strata,which is at risk of firedamp explosion that usually results in disaster.Airtight concrete grouting is popularly used in this kind of tunnel project,Based on the essential theory of mechanics of fluids in prous media,the principle of improving concrete airtight property and its influential factors are investigated.The proportioning tests and monitoring method for airtight concrete are introduced,which is illustrated by a case study applied to the project of the Huayinshan Tunnel.It is proved by engineering practices that the achievement of this research work is beneficial to tunneling project across coal strata.