Biofunctionalized nanoslits for wash-free and spatially resolved real-time sensing with full target capture

We propose biofunctionalized nanofluidic slits (nanoslits) as an effective platform for real-time fluorescence-based biosensing in a reaction-limited regime with optimized target capture efficiency. This is achieved by the drastic reduction of the diffusion length, thereby a boosted collision frequency between the target analytes and the sensor, and the size reduction of the sensing element down to the channel height comparable to the depletion layer caused by the reaction. Hybridization experiments conducted in DNA-functionalized nanoslits demonstrate the analyte depletion and the wash-free detection ∼10 times faster compared to the best microfluidic sensing platforms. The signal to background fluorescence ratio is drastically increased at lower target concentrations, in favor of low-copy number analyte analysis. Experimental and simulation results further show that biofunctionalized nanoslits provide a simple means to study reaction kinetics at the single-pixel level using conventional fluorescence microscopy with reduced optical depth.     

对称反循环矩阵的逆矩阵

讨论了求对称反循环矩阵逆矩阵的一般方法,并根据反循环矩阵与对称反循环矩阵的特殊关系给出几类特殊对称反循环矩阵的逆矩阵.     

CRP,but not TNF-α or IL-6, decreases after weight loss in patients with morbid obesity exposed to intensive weight reduction and balneological treatment

Objective

The aim of this study was to evaluate the concentrations of C-reactive protein (CRP), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and the degree of homeostasis model assessment-insulin resistance (HOMA-IR) in patients with morbid obesity exposed to a three-week low-calorie diet and balneotherapy.

Methods

The study included 33 patients (25 females and 8 males; mean age 46 years) with body mass index (BMI) values of >40 kg/m². Evaluations of CRP, IL-6, TNF-α, lipid profile, HOMA-IR, and fasting glucose were carried out before (baseline data) and three weeks after the treatment. The control group consisted of 20 healthy volunteers (15 females and 5 males) with a mean age of 39 years and BMI values of ≤24.9 kg/m².

Results

In the blood of patients with morbid obesity we found significantly elevated levels of CRP, TNF-α, triglycerides, HOMA-IR and fasting glucose, but a decreased level of high density lipoprotein (HDL)-cholesterol, compared with the healthy individuals. The treatment resulted in about a 9.4% reduction in body weight from 122.5 to 111.0 kg and a significant decrease in the concentration of CRP, but no change in TNF-α or IL-6. HOMA-IR was significantly reduced.

Conclusion

The decrease in CRP level without changes in TNF-α or IL-6 concentrations after the low-calorie diet and balneological treatment, suggests that an essential amount of adipose tissue must be removed before proper adipocyte function is restored. The decrease in HOMA-IR indicates an improvement in insulin sensitivity, which is beneficial in obese patients.

     

Sustainability Strategies in the Forestry–Wood Chain Driven by Market Demands on Books and Other Products

This paper focuses on sustainability strategies for the Forestry–Wood chain (FWC) driven by market demands on books and other products. The main objective is to draft and suggest a process for market driven pro-active sustainability strategies for the FWC, based on a SWOT-analysis (strengths, weaknesses, opportunities and threats). Further to highlight the possible strategic implications and critical areas for actors upstream in the FWC, in the context of uncertain external future scenarios. Higher demands on product differentiation were found throughout the FWC, where also collaboration and stakeholder interaction was found important to improve sustainability. The work broadens the “traditional” perspectives on FWC sustainability to encompass also interacting value chains such as book production and publishing, to get a more holistic view.     

基于IPSeC的移动IP安全技术

随着通信技术的快速发展以及人们对通信终端移动性的强烈要求,移动IP的出现就成为了必然.对IPSec 在移动IP中的应用提高移动IP的通信安全性进行了具体的分析,并简述了移动IP技术的未来发展前景.     

外语学习语言共同体的行为分析及其应用

语言共同体中进行语言行为分析,并将其与外语学习结合起来,从不同角度揭示语言学习属性.本文引证美国行为学最近成果,从深奥复杂的行为分析理论中提炼出应用语言学和语言行为学之间的互证性,以求进一步实践探索.     

Young Diplomats?? Socialization to the Networked Professional Cultures of Their Workplace Communities

The purpose of the present investigation was to examine young diplomats’ socialization to the professional expert culture of the Ministry for Foreign Affairs of Finland over a six-month on-job training period, as part of their preparation for service in the diplomatic corps. Using social network analysis, we analyzed departments’ internal social structures, prevailing social relations, and the young diplomats’ role and position in the networks of knowledge acquisition, practical know-how, and professional collaboration. Data collected by contextual event sampling and theme interviews and analyzed by qualitative content analysis were used to characterize the young diplomats’ personal social support networks, resources obtained, as well as associated reflections regarding their functioning in and socialization to the departmental workplace community. The results indicated that across the six-month training period, the young diplomats became involved to the workplace communities’ networked expertise and were socialized to its expert culture, even if their achieved networking positions differed. The results revealed differences between levels of collective operational practices in the workplace communities as well as the nature of assignments in which the young diplomats participated and for which they were responsible. It was proposed that these differences mirrored distinction between expansive natures of young diplomats’ workplace communities as learning environments.     

Learning by Interest: Experiences and Commitments in Lives with Dance and Crafts

In this article we intend to contribute to the understanding of interest-driven learning which occurs when people find and choose an interest of their own. This engagement continues over a long time and includes development and learning. The empirical material consists of 14 learning stories written by experienced and skilled informants in folk dance and different crafts. The purpose is to illuminate the diversity and complexity of learning and meaning the informants assign to dance or crafts. This is what we consider to be the perspective of learning by interest. We also argue for the importance of understanding learning by interest as embedded in cultural heritage and social and cultural changes. Theoretically, we draw on John Dewey (1913/2009; 1934/2005). Learning folk dance or crafts is largely a question of learning by doing, which makes experience important. The interest and effort involved can in our interpretation be understood as experiences where expressions of commitment are embedded. The empirical analysis highlights three emphases in this illumination of the learning process. The first considers the individual development of skill in relation to broadened learning of collective tasks. The second perspective focuses on the interplay between learning in a life course and significant learning experiences. The third concerns the interplay between personal meaning and craftsmanship. In sum, this paper contributes to the understanding of learning by interest as a useful concept. In the final discussion we expand our perspective since the contrast is obvious between learning by interest and interpretations of the contemporary society, where individualization and lack of commitment is stressed.     

Preparation of carbon encapsulated iron nanoparticles with very thin shells by DC arc discharge

Carbon encapsulated iron nanoparticles(CEINPs) with very thin shells and good core-shell structures were prepared by DC arc discharge at argon intake temperature(AIT) of 800 ℃. The results of high resolution transmission electron microscope(HRTEM), energy dispersive X-ray(EDX) spectroscope, X-ray diffraction(XRD), and X-ray photoelectron spectroscope(XPS) characterizations on the product B show that the thickness of the carbon shells of CEINPs in the product B is in the range of ca. 0.5—5.3 nm, i. e., which can be as thin as only two layers of graphite. The average diameter of the CEINPs is about 24. 7 nm. The total content of Fe element in the product B is 77.0 wt%. The saturation magnetization(Ms) and coercivity(Hc) of the product B are 107.4 emu/g and 143 Oe, respectively. The formation of the CEINPs in the product B is discussed briefly.