A rapid and accurate 3-（4,5-dimethyl thiazol-2-yl）-2,5-diphenyl tetrazolium bromide colorimetric assay for quantification of bacteriocins with nisin as an example

The objective of this study is to propose a more accurate and faster MTT 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetric assay (MCA) for quantitative measurement of polypeptide bacteriocins in solutions with nisin as an example. After an initial incubation of nisin and indicator bacterium Micrococcus luteus NCIB 8166 in tubes, MTT was added for another incubation period. After that, nisin was quantified by estimating the number of viable bacteria based on measuring the amount of purple formazan produced by cleavage of yellow tetrazolium salt MTT. Then MCA was compared to a standard agar diffusion assay (ADA). The results suggested a high correlation coefficient (r2=0.975±0.004) between optical density (OD) and the inhibitory effect of nisin on a bacterial strain Micrococcus luteus NCIB 8166 at a range of 0.125～32 IU/ml. The MCA described in this study was very quick. Quantification of nisin took only 7～8 h and the detection limit was at the level of 0.125 IU/ml when compared to 12 IU/ml and 24～28 h for ADA. The MCA provides an accurate and rapid method for quantifi-cation of nisin in solutions and is expected to be used for quantification of other antimicrobial substances.     

职技高师进入经济建设主战场的实践与探索

如何利用人才和科技优势为经济建设主战场服务是高等学校面临的重要课题,本文着重介绍近年来我院开展的主要工作,并对有关问题进行探索。     

必须充分认识与素质教育相关的五个问题

近年来,广大教育工作和学生所热切期盼的素质教育现已成为全社会的共识。本试图从外在因素的角度来探讨一下素质教育的表层问题,据此可以找出现有教育中那些明显不符合素质教育的东西。     

《马戏团之夜》的叙事视角分析

本文试图通过细读法对英国当代著名女作家安吉拉·卡特的代表作《马戏团之夜》进行文本解读,作家在该小说中对不同类型叙事视角的运用大胆灵活而又恰到好处,既满足了故事情节发展的需要,也体现了作家激进而独特的创作风格。     

企业文化与企业发展及企业文化的培育

企业化是潜在的生产力,是企业员工共同的精神支柱,它对企业的发展起着不可替代的重大作用。在经济体制改革不断深入的今天,更应注重培育和建设企业化。     

普通中专大学化转型的三种基本模式及问题分析

世纪之交,高等教育大众化步伐的加快使曾一度作为高等教育补充形式普通中专教育面临十分尴尬和困难的境地,“大学化”的教育升级和转型开始成为普通中专应对危机的主要选择途径,盘活了教育资源。职业技术学院、社区性高职学院、中专、专科并入高校这三种基本模式实际都是中专办学独立状态的历史性消失,中专“大学化”后过渡期问及其以后面临的问题必须引起足够重视。     

Does STES‐Oriented Science Education Promote 10th‐Grade Students’ Decision‐Making Capability?

Today’s society is continuously coping with sustainability‐related complex issues in the Science‐Technology‐Environment‐Society (STES) interfaces. In those contexts, the need and relevance of the development of students’ higher‐order cognitive skills (HOCS) such as question‐asking, critical‐thinking, problem‐solving and decision‐making capabilities within science teaching have been argued by several science educators for decades. Three main objectives guided this study: (1) to establish “base lines” for HOCS capabilities of 10th grade students (n = 264) in the Israeli educational system; (2) to delineate within this population, two different groups with respect to their decision‐making capability, science‐oriented (n = 142) and non‐science (n = 122) students, Groups A and B, respectively; and (3) to assess the pre‐post development/change of students’ decision‐making capabilities via STES‐oriented HOCS‐promoting curricular modules entitled Science, Technology and Environment in Modern Society (STEMS). A specially developed and validated decision‐making questionnaire was used for obtaining a research‐based response to the guiding research questions. Our findings suggest that a long‐term persistent application of purposed decision‐making, promoting teaching strategies, is needed in order to succeed in affecting, positively, high‐school students’ decision‐making ability. The need for science teachers’ involvement in the development of their students’ HOCS capabilities is thus apparent.     

合并馆藏书排架问题的探索

多馆图书合并之后如何科学而有序的确定组织排架是一个不可回避的现实问题,另起炉灶、分片划块、大归类小集中、保一改二等均有其利弊得失,改编工作势在必行,要抓住目录、索书号、根卡以及排架归类、定架定位等几个环节,以少归多,摸索前进。     

依法治国背景下的地方法治建设

在阐述依法治国、地方法治建设及其相互关系的基础上，审视地方法治社会建设发展的历史与现状，分析中国目前地方法治社会建设的主要障碍及成因，并提出全面推进依法治国背景下加强地方法治社会建设的对策。