人纤维蛋白原α、β、γ基因的克隆和表达研究

纤维蛋白原(fibrinogen,Fg)是凝血因子I,在体内经凝血酶(thrombin)作用下转变为纤维蛋白,发挥其凝血和止血功能。本研究通过反转录的方法,从杭州人肝脏中成功克隆到人纤维蛋白原α、β、γ基因,并将其定向插入原核表达载体中,人纤维蛋白原α、β、γ基因经成功诱导获得表达,进一步纯化获得人纤维蛋白原α、β、γ蛋白。序列经比对,与Genbank上公布的序列同源性达97%～99%,这项研究为我国汉族人群纤维蛋白原基因核苷酸多态性和单倍型研究发挥一定作用,并且对纤维蛋白封闭剂的开发,提供了重要的产业化实验依据和技术基础。     

日语初级阶段教学中的文化导入

语言与文化密不可分,教授语言其实是在教授文化。日语初级阶段的学习十分重要,将日本文化融入到这个阶段的教学中,能够有效帮助学生取得良好的学习效果,更好地进行跨文化交流。本文针对高校日语初级阶段教学的特点,探讨了在日本文化背景下进行日语教学的重要性,并提出了一些文化导入的内容与方法。     

一种基于laplace变换求解跳变问题的新方法

信号与系统课程是电子信息类专业的核心基础课程,在各种经典教材中都采用冲激响应匹配法求解微分方程零时刻的跳变值。本文分析了冲激响应匹配法求解跳变问题存在的问题,提出了一种应用laplace变换求解跳变问题的新方法,简述了此种方法的原理和求解过程。多年的教学经验表明这种方法更简单,更易让学生掌握。     

浅析高校档案管理的信息化建设策略

高校的档案管理是人力资源部门的基本工作之一,亦是人事管里中不能缺少的一个环节。伴随高等院校教学事业的日益前进,高等院校档案管理工作存有的问题也日益凸显,面临的挑战也愈来愈多。对于网络化的时代背景下,如何加强高校档案管理的信息化建设提出思考。     

略论孟称舜杂剧《泣赋眼儿媚》

《泣赋眼儿媚》是明代戏曲家孟称舜早年创作的爱情题材的杂剧。该剧取材于元代蒋子正《山房随笔》中所记载的陈诜与妓女江柳的爱情故事,塑造了一个理性而勇于追求爱情的妓女江柳的形象,语言本色而有诗意。该剧的缺憾是戏剧冲突不够激烈,情节稍显平实。     

认知神经科学在数字阅读研究中的应用与展望

采用认知神经科学研究数字阅读,本质上是通过获取读者认知过程与情感体验的神经数据,实现数字出版从创作、阅读、发行到传播全过程的科学研究.本文梳理认知神经科学常用的技术方法与多领域的研究应用,以及当前在数字阅读体验、效果、过程、机制研究四个方面应用的现状与不足;进而围绕数字阅读研究中读者偏好、作品优化、发行预测、传播规律四大难点问题,提出全新的研究方法、研究思路,构建基于认知神经科学的数字阅读研究框架,以期打开"黑箱",探索"神经出版学"的理论创新与学科发展.     

Relooking Assessment: A Study on Assessing Developmental Learning Outcomes in Toddlers

In most countries the funding for early childhood education has increased and governments in some countries have taken serious steps to bring about positive change in the profession. However, the increase in funding by governments and other funding organisations around the world has, understandably, attracted increased accountability as these organisations need to know that their financial investments are achieving desired outcomes. To seek evidence that positive learning outcomes have indeed been achieved through these investments is a reasonable request, and there is a shared responsibility and accountability for professionals to provide appropriate evidence. The downside, however, can be the request for standardised test information, as if performance on such tests provides proof of all desired outcomes. More than ever before, it is important for early childhood educators to be able to provide accurate, objective information about children’s assessment in ways other than by standardised testing, which may not reflect the complex reality of children’s lives. This paper reports on a research study in Singapore that investigated curriculum effectiveness using developmental learning outcomes as a means of assessing children. The research was devised to examine if eight specified broad developmental learning outcomes could measure the effectiveness of the curriculum by assessing children’s learning as shown in qualitative data. Practical examples showed evidence of children’s learning and the role of the educator in facilitating and documenting developmental learning outcomes.     

学生购彩意向的影响因素研究——体育彩民学生与非体育彩民学生比较

采用自编学生购彩意向影响因素调查问卷,对我国10个省(市)的123名体育彩民学生和628名非体育彩民学生购彩意向的影响因素进行比较研究.结果表明:体育彩民学生的经济效用得分显著高于非彩民学生;男性非彩民学生的风险偏好得分显著高于女性非彩民学生;家人购彩对学生购彩认知和外部信息作用因子有影响.应培养学生树立良好的价值观,弱化彩票的经济效用;预防和干预学生追求"刺激性"的购彩心理;营造良好的家庭氛围,使学生理性对待购彩.     

ENSO and Southeast Asian biomass burning modulate subtropical trans-Pacific ozone transport

Trans-Pacific transport of enhanced ozone plumes has been mainly attributed to fossil fuel combustion in Asia in spring, but less attention has been paid to vegetation fires in Asia. Here we show that the El Niño-Southern Oscillation (ENSO)-modulated fires in Southeast Asia, rather than Asian fossil fuel plumes, dominate the interannual variability of springtime trans-Pacific transport of ozone across the entire North Pacific Ocean. During El Niño springs, the intensified fires from both the Indochinese Peninsula and Indonesia, together with large-scale circulation anomalies, result in enhanced ozone plumes that stretch over 15 000 km in both the lower-middle and upper troposphere. This enhancement is also observed in the in situ measurements of ozone concentration, with an almost 10% increase at Mauna Loa Observatory, Hawaii, a unique site to monitor the long-distance transport over the North Pacific. This study reports an unexpectedly strong influence of vegetation fires, linked with climate variability, on global tropospheric chemistry and proves once more how complex the interactions in the climate system are.