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191.
In this paper, we present a microfluidic chip that is capable of measuring electrical conductance through gap junction channels in a 2-dimensional cell sheet. The chip utilizes a tri-stream laminar flow to create a non-conductive sucrose gap between the two conducting solutions so that electrical current can pass across the sucrose gap only through the cells. Using the chip, we tested the effect of a gap junction inhibitor, 2-APB, on the electrical coupling of connexin 43 (Cx43) gap junction channels in NRK-49F cells. We found that 2-APB reversibly blocks the conductivity in a dose-dependent manner. The tri-stream chip further allows us to simultaneously follow the conductance changes and dye diffusion in real time. We show that 2-APB affects both conductance and diffusion, supporting the interpretation that both sets of data reflect the same gap junction activity. The chip provides a generic platform to investigate gap junction properties and to screen drugs that may inhibit or potentiate gap junction transmission. 相似文献
192.
The purpose of the present study was to analyse the variability in clubhead presentation to the ball and the resulting ball impact location on the club face for a range of golfers of different ability. A total of 285 male and female participants hit multiple shots using one of four proprietary drivers. Self-reported handicap was used to quantify a participant's golfing ability. A bespoke motion capture system and user-written algorithms was used to track the clubhead just before and at impact, measuring clubhead speed, clubhead orientation, and impact location. A Doppler radar was used to measure golf ball speed. Generally, golfers of higher skill (lower handicap) generated increased clubhead speed and increased efficiency (ratio of ball speed to clubhead speed). Non-parametric statistical tests showed that low-handicap golfers exhibit significantly lower variability from shot to shot in clubhead speed, efficiency, impact location, attack angle, club path, and face angle compared with high-handicap golfers. 相似文献
193.
Betzler NF Monk SA Wallace ES Otto SR 《Sports biomechanics / International Society of Biomechanics in Sports》2012,11(2):223-238
The aim of this study was to quantify and explain the effect of shaft stiffness on the dynamics of golf drives. Twenty golfers performed swings with two clubs designed to differ only in shaft bending stiffness. Wrist kinematics and clubhead presentation to the ball were determined using optical motion capture systems in conjunction with a radar device for capturing ball speed, launch angle, and spin. Shaft stiffness had a marginally small effect on clubhead and ball speeds, which increased by 0.45% (p < 0.001) and 0.7% (p = 0.008), respectively, for the less stiff club. Two factors directly contributed to these increases: (i) a faster recovery of the lower flex shaft from lag to lead bending just before impact (p < 0.001); and (ii) an increase of 0.4% in angular velocity of the grip of the lower flex club at impact (p = 0.003). Unsurprisingly, decreases in shaft stiffness led to more shaft bending at the transition from backswing to downswing (p < 0.001). Contrary to previous research, lead bending at impact marginally increased for the stiffer shaft (p = 0.003). Overall, and taking effect sizes into account, the changes in shaft stiffness in isolation did not have a meaningful effect on the measured parameters, for the type of shaft investigated. 相似文献
194.
综述了养老保险基金收入的结构情况,建立了养老保险基金的数学模型,分析了其中的几个主要因素:退保率和工资增长率。利用模型对养老保险基金的收入情况进行预测,对基金运营的参数基金敏感性进行分析。 相似文献
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198.
Recent developments in Nancy Cartwright's philosophy are examined, against the background of her earlier ideas. The examination is structured around two related problems for the interpretation of Cartwright; how to understand Cartwright's claim that we can see capacities in experiments, and the question of whether Cartwright is entitled to call herself an empiricist. It is argued that we should understand Cartwright as allowing that the seeing of capacities in experiments can incorporate an interpretive aspect. It is further argued that, although Cartwright is a long way from contemporary Humean empiricists, her views can be understood as falling within the broad empiricist tradition. In the process of addressing these issues the role of idealization in Cartwright's thought is explained. 相似文献
199.
作者在文中反驳了科学只能描述不能解释的观点,并结合物理学背景阐释了科学解释的含义。在文章的最后,作者指出了科学解释的限度,像偶然事件、道德规范、终极理论,都超出了科学解释的能力。 相似文献
200.
Kenny IC Wallace ES Otto SR 《Sports biomechanics / International Society of Biomechanics in Sports》2008,7(3):322-332
The aim of this study was to determine how shaft length affects golf driving performance. A range of drivers with lengths between 1.168 m and 1.270 m, representing lengths close to the 1.219 m limit imposed by R&A Rules Limited (2008), were assembled and evaluated. Clubhead and ball launch conditions and drive distance and accuracy were determined for seven category 1 golfers (handicaps 0.21 +/- 2.41) who performed shots on a purpose-built practice hole. As shaft length increased from 1.168 m to 1.270 m, initial ball velocity increased (+ 1.8 m/s, P < 0.01). Ball carry (+ 4.3 m, P = 0.152) also increased, although not significantly so. Furthermore, as shaft length increased, for all club comparisons there was no decrease in accuracy. Ball launch conditions of spin components and launch angle remained unaffected by shaft length. Launch angle increased (0.8 degree, F = 1.074, P = 0.362) as driver shaft length increased. Our results show that clubhead and ball velocity together with ball carry tended to increase with no loss of accuracy. 相似文献