兰州大学研究生院“研究方法”课程的开展现状及对策研究

采用调查、访谈和逻辑分析等研究方法,对兰州大学研究生院研究方法课程开展的现状进行调查与分析,探究制约其开展的主要因素,提出相应的建议,以期推动甘肃省乃至全国研究方法课程的开展。     

东北地区经济发展与环境保护研究

随着振兴东北老工业基地战略的实施,对环境提出了更高的要求,试分析东北地区经济、社会与环境面临的挑战和机遇,指出走绿色、循环经济的道路,并提出合理的建议。     

基于捕获功能的电压频率及相序检测方法

本文提出一种利用TMS320LF2407控制器的捕获功能对变频器输出电压的频率及相序进行检测的方法.实验结果表明使用TMS320L[2407的捕获功能可以很好地检测出变频器输出电压的频率大小及相位顺序,该方法对硬件要求低,实现简单、应用性强.     

论思想政治教育学基本理论研究

我国在制度上已经建立了思想政治教育学科,现在需要重视思想政治教育学科的基本理论建设,这是获得学术界的学科认同、对社会发展和科学发展发挥作用、从学术意义上建立思想政治教育学科的基础。思想政治教育学基本理论研究,包括基础理论、基本问题、核心范畴、基本精神、基本特征、基本方法、历史、范式、经典、方法论等问题,应从学术层面做出科学的回答,充实思想政治教育学知识库。     

近十五年来高校德育社会化研究综述

高校德育社会化研究是高校德育领域的一个前沿性课题,20世纪90年代中期以来,高校德育社会化问题开始引起学者们的关注,并逐渐走进学术研究的视野。到目前为止,学术界对高校德育社会化的内涵、必然性、存在的问题和实现途径进行了初步的探讨,取得了一定的成果,但仍需进一步深化高校德育社会化的理论研究和实践探索。     

用疑问方法激活思想政治理论课

用疑问方式激活思想政治教育是上好政治课的好方法。要落实好：重设疑、善思疑、释好疑三个因素,促使思想政治课取得更好效果。     

Mathematical models of canine right and left atria cardiomyocytes

The aim of this study is to build two mathematical models of canine ionic currents specific to right atria and left atria.The canine left atria mathematical model was firstly modified from the Ramirez-Nattel-Courtemanche(RNC) model using the recently available experimental data of ionic currents and was further developed based on our own experimental data.A model of right atria was then built by considering the differences between right atria and left atria.The two developed models well reproduced the exper...     

Heat shock protein 90 protects rat mesenchymal stem cells against hypoxia and serum deprivation-induced apoptosis via the PI3K/Akt and ERK1/2 pathways

Mesenchymal stem cell(MSC)transplantation has shown a therapeutic potential to repair the ischemic and infracted myocardium,but the effects are limited by the apoptosis and loss of donor cells in host cardiac microenvironment.The aim of this study is to explore the cytoprotection of heat shock protein 90(Hsp90)against hypoxia and serum deprivation-induced apoptosis and the possible mechanisms in rat MSCs.Cell viability was determined by3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay.Apoptosis was assessed by Hoechst 33258nuclear staining and flow cytometric analysis with annexin V/PI staining.The gene expression of Toll-like receptor-4(TLR-4)and V-erb-b2 erythroblastic leukemia viral oncogene homolog 2(ErbB2)was detected by real-time polymerase chain reaction(PCR).The protein levels of cleaved caspase-3,Bcl-2,Bcl-xL,Bax,total-ERK,phospho-ERK,totaI-Akt,phospho-Akt,and Hsp90 were detected by Western blot.The production of nitric oxide was measured by spectrophotometric assay.Hsp90 improves MSC viability and protects MSCs against apoptosis induced by serum deprivation and hypoxia.The protective role of Hsp90 not only elevates Bcl-2/Bax and Bcl-xL/Bax expression and attenuates cleaved caspase-3 expression via down-regulating membrane TLR-4 and ErbB2 receptors and then activating their downstream PI3K/Akt and ERK1/2 pathways,but also enhances the paracrine effect of MSCs.These findings demonstrated a novel and effective treatment strategy against MSC apoptosis in cell transplantation.     

Clinicopathological features of atypical lipomatous tumors of the laryngopharynx

Atypical lipomatous tumor (ALT) of the laryngopharynx is rare. Here we report five cases to demonstrate their clinicopathological features. The patients were four males and one female, aged 41 to 69 years (median 53.6 years). All tumors (two in the hypopharynx and three in the larynx) presented as a slowly growing, painless mass. Symptoms included dysphagia (2/5), dysphonia (3/5), and the feeling of a foreign body in the throat (5/5). Tumors were well circumscribed or focally infiltrative, ranging from 2.0 to 5.0 cm (median, 3.4 cm) in size, and microscopically showed the typical features of lipoma-like ALT. Immunohistochemically, tumor cells were stained with S-100, vimentin, murine double minute 2 (MDM-2), and cyclin-dependent kinase 4 (CDK4). Two patients had local tumor recurrences at 6 and 14 months after initial surgery during follow-up. ALT of laryngopharynx is an indolent tumor. Immunohistochemical staining for MDM-2 and CDK4 is helpful in pathological diagnosis.