Effects of purposeful soccer heading on circulating small extracellular vesicle concentration and cargo

BackgroundConsidering the potential cumulative effects of repetitive head impact (HI) exposure, we need sensitive biomarkers to track short- and long-term effects. Circulating small extracellular vesicles (sEVs) (<200 nm) traffic biological molecules throughout the body and may have diagnostic value as biomarkers for disease. The purpose of this study was to identify the microRNA (miRNA) profile in circulating sEVs derived from human plasma following repetitive HI exposure.MethodsHealthy adult (aged 18–35 years) soccer players were randomly assigned to one of 3 groups: the HI group performed 10 standing headers, the leg impact group performed 10 soccer ball trapping maneuvers over 10 min, and the control group did not participate in any soccer drills. Plasma was collected before testing and 24 h afterward, and sEVs were isolated and characterized via nanoparticle tracking analysis. Next-generation sequencing was utilized to identify candidate miRNAs isolated from sEVs, and candidate microRNAs were analyzed via quantitative polymerase chain reaction. In silico target prediction was performed using TargetScan (Version 7.0; targetscan.org) and miRWalk (http://mirwalk.umm.uni-heidelberg.de/) programs, and target validation was performed using luciferase reporter vectors with a miR-7844-5p mimic in human embryonic kidney (HEK) 293T/17 cells.ResultsPlasma sEV concentration and size were not affected across time and group following repetitive HI exposure. After 24 h, the HI read count from next-generation sequencing showed a 4-fold or greater increase in miR-92b-5p, miR-423-5p, and miR-24-3p and a 3-fold or greater decrease in miR-7844-5p, miR-144-5p, miR-221-5p, and miR-22-3p. Analysis of quantitative polymerase chain reaction revealed that leg impact did not alter the candidate miRNA levels. To our knowledge, miR-7844-5p is a previously unknown miRNA. We identified 8 miR-7844-5p mRNA targets: protein phosphatase 1 regulatory inhibitor subunit 1B (PPP1R1B), LIM and senescent cell antigen-like domains 1 (LIMS1), autophagy-related 12 (ATG12), microtubule-associated protein 1 light chain 3 beta (MAP1LC3B), integrin subunit alpha-1 (ITGA1), mitogen-activated protein kinase 1 (MAPK1), glycogen synthase kinase 3β (GSK3β), and mitogen-activated protein kinase 8 (MAPK8).ConclusionCollectively, these data indicate repetitive HI exposure alters plasma sEV miRNA content, but not sEV size or number. Furthermore, for the first time we demonstrate that previously unknown miR-7844-5p targets mRNAs known to be involved in mitochondrial apoptosis, autophagy regulation, mood disorders, and neurodegenerative disease.     

The relationship between peak height velocity and physical performance in youth soccer players

Longitudinal changes in height, weight and physical performance were studied in 33 Flemish male youth soccer players from the Ghent Youth Soccer Project. The players' ages at the start of the study ranged from 10.4 to 13.7 years, with a mean age of 12.2 +/- 0.7 years. Longitudinal changes were studied over a 5 year period. Peak height velocity and peak weight velocity were determined using non-smoothed polynomials. The estimations of peak height velocity, peak weight velocity and age at peak height velocity were 9.7 +/- 1.5 cm x year-1, 8.4 +/- 3.0 kg x year-1 and 13.8 +/- 0.8 years, respectively. Peak weight velocity occurred, on average, at the same age as peak height velocity. Balance, speed of limb movement, trunk strength, upper-body muscular endurance, explosive strength, running speed and agility, cardiorespiratory endurance and anaerobic capacity showed peak development at peak height velocity. A plateau in the velocity curves was observed after peak height velocity for upper-body muscular endurance, explosive strength and running speed. Flexibility exhibited peak development during the tear after peak height velocity. Trainers and coaches should be aware of the individual characteristics of the adolescent growth spurt and the training load should also be individualized at this time.     

An On-line Search for Optimum Operating Conditions of Noisy Systems

Optimum process operating conditions are often achieved by on-line computer programming. A general method has been developed which is capable of optimizing processes whose responses are corrupted by random error. The method is particularly useful for application to processes whose response functions contain sharp ridges. Application to ridge and non-ridge systems gives the same results in each case showing that the rate of convergence of the search is totally unaffected by the presence of a ridge. An accelarated gradient method is used which obtains its derivatives from a quadratic interpolation. The inability of the gradient method to move along a ridge is overcome by projecting a straight line through points on the ridge to obtain new starting points for a gradient search. Points on the ridge are located by finding the stationary point of the polynomial which is constructed by regression through points found by the gradient search. The method is applied to several test functions known to the literature—a fast convergence rate is obtained for a wide range of noise levels.     

The physics of brass wind instruments

The design of musical instruments owes more to art than to science and assessment of their quality is essentially subjective. Nevertheless, the physical principles of their operation are very interesting and may be of practical importance. This article reviews the acoustics of brass wind instruments, regarding the player and his instrument as an entity, and compares experimental and theoretical results.     

Three-axis platform simulation: Bond graph and Lagrangian approach

A bond graph model is derived for the geometric constraints of a three-axis flight table. Gimbal dynamics are easily added even in asymmetrical and unbalanced cases. A method is introduced to make the local dependent inertias computable. The bond graph compares favourably to the Lagrangian approach as to modelling effort and accessibility of intermediate variables as well as having computational advantages.     

Ladder realizations of multivariable positive real functions

Necessary and sufficient conditions are obtained for the realization of an m-variable positive real function (PRF) as the impedence function of a resistively-terminated ladder network of m lossless two-ports connected in cascade. Each two-port is a single-variable lossless ladder with all of its transmission zeros either at the origin or at finity. Conditions are also obtained when each of the two-ports is a Fujisawa-type lowpass ladder.