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171.
Jeonghun Nam Justin Kok Soon Tan Bee Luan Khoo Bumseok Namgung Hwa Liang Leo Chwee Teck Lim Sangho Kim 《Biomicrofluidics》2015,9(6)
A novel microfluidic device which consists of two stages for particle focusing and separation using a viscoelastic fluid has been developed. A circular capillary tube was used for three-dimensional particle pre-alignment before the separation process, which was inserted in a polydimethylsiloxane microchannel. Particles with diameters of 5 and 10 μm were focused at the centerline in the capillary tube, and the location of particles was initialized at the first bifurcation. Then, 5 and 10 μm particles were successfully separated in the expansion region based on size-dependent lateral migration, with ∼99% separation efficiency. The proposed device was further applied to separation of MCF-7 cells from leukocytes. Based on the cell size distribution, an approximate size cutoff for separation was determined to be 16 μm. At 200 μl/min, 94% of MCF-7 cells were separated with the purity of ∼97%. According to the trypan blue exclusion assay, high viability (∼90%) could be achieved for the separated MCF-7 cells. The use of a commercially available capillary tube enables the device to be highly versatile in dealing with particles in a wide size range by using capillary tubes with different inner diameters. 相似文献
172.
173.
金沙方言属于西南官话川黔片成渝小片,虽然其基本特点与普通话一致性较高,但其语音、词汇、语法与普通话都存在一定的差异。就语音方面的声母看,两者共同的声母有17个,普通话特有的声母有5个,金沙方言特有的声母有3个。金沙方言特有的声母与普通话有比较复杂的对应关系,厘清这种对应关系,对于金沙方言区的人学习和推广普通话具有一定现实意义。 相似文献
174.
谭本超 《安顺师范高等专科学校学报》2015,17(2)
欠发达地区新建地方高校的大学生英语口语学习始终难以克服本土化困境,如语言环境差、高中英语学习的惯性、特殊的心理障碍、教育评价系缺失等不利因素一直制约着他们英语口语学习成效的提高.大学生们只有针对这些在在的问题,有针对性地加以解决,才能提高英语口语的交际能力. 相似文献
175.
Yue ZHANG Hai-peng LV Cheng-ying MA Li GUO Jun-feng TAN Qun-hua PENG Zhi LIN 《Journal of Zhejiang University. Science. B》2015,16(2):103-112
目的:从茶树中克隆一种可以催化表没食子儿茶素没食子酸酯(EGCG)生成甲基化EGCG的酶——咖啡酰辅酶A氧甲基转移酶(CCo AOMT),实现甲基化EGCG的酶学合成,为甲基化EGCG的进一步开发利用提供理论依据和技术指导。创新点:本研究首次从茶树中克隆了一条CCo AOMT基因组序列;分析了CCo AOMT基因在不同茶树品种和不同成熟度茶鲜叶中的基因表达规律;证明了CCo AOMT具有催化合成甲基化EGCG的生物活性。方法:采用聚合酶链式反应(PCR)和序列分析获得CCo AOMT的编码序列和基因组序列;采用高效液相色谱-四级杆-飞行时间串联质谱技术(HPLC-QTOF-MS)分析酶促反应生成的甲基化EGCG产物(图4);采用实时荧光定量PCR分析CCo AOMT基因的表达差异(图5)。结论:本研究从茶树中克隆了CCo AOMT基因的编码序列(738 bp)和基因组序列(2678 bp),明确了该基因具有4个内含子和5个外显子;揭示了CCo AOMT可以催化EGCG生成EGCG4"Me、EGCG3"Me和EGCG3’Me等多种甲基化产物;证明了CCo AOMT具有催化生成甲基化EGCG的活性;并发现该基因的表达量高低与茶鲜叶的成熟度呈正相关关系。 相似文献
176.
Due to their toxicity, the increased distribution of microcystins (MCs) has become an important worldwide problem. MCs have
been recognized as inhibitors of protein phosphatase 2A (PP2A) through their binding to the PP2A catalytic subunit. However,
the exact mechanism of MC toxicity has not been elucidated, especially concerning the cellular response and its autoregulation.
To further dissect the role of PP2A in MC-induced toxicity, the present study was undertaken to determine the response of
PP2A in human amniotic epithelial (FL) cells treated with microcystin-LR (MCLR), one of the MC congeners. The results show
that a low-dose treatment of MCLR in FL cells for 6 h induced an increase in PP2A activity, and a high-dose treatment of MCLR
for 24 h decreased the activity of PP2A, as expected. The increased mRNA and protein levels of the PP2A C subunit may explain
the increased activity of PP2A. Furthermore, MCLR altered microtubule post-translational modifications through PP2A. These
results further clarify the underlying mechanism how MCLR affects PP2A and may be helpful for elucidating the complex toxicity
of MCLR. 相似文献
177.
The Bacillus strain BH072 isolated from a honey sample showed strong antifungal activity against phytopathogen. Gene cloning test demonstrated that the strain had a tasA gene encoding an antifungal TasA protein. Although the wild strain simultaneously produced various antifungal substances, only the physicochemical property and antifungal activity of TasA protein were unclear due to the difficulty in extraction. In this study, tasA gene encoding the protein from Bacillus sp. BH072 was amplified by using the polymerase chain reaction (PCR) method and cloned into pET 28a (+) vector, and then expressed in host cells Escherichia coli BL21 (DE3). The expressed proteins were collected by centrifugation and ultrasonic treatment, and then purified by using nickel-nitrilotriacetic acid (Ni-NTA) metal affinity column and dialysis methods. The result of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) test showed that an expected protein band appeared with a size of 31 kDa. The expressed products possessed antifungal activity against the phytopathogenic indicator strain Botrytis cinerea. A genetically engineered strain tasA of E. coli was established in this study which can efficiently express Tas A protein. 相似文献
178.
以过量表达 AtHsfAla 及 AtHsfAla 基因沉默的转基因植株为材料,通过控制土壤水分进行干旱处理,结果显示,过量表达型干旱处理后的存活率最大,而基因沉默型最小,表明 AtHsfA1a 能增强植物的抗旱性。为了从生理水平研究 AtHsfA1a 提高抗旱性的机制,研究发现过量表达型的叶片相对含水量和叶绿素含量最高,而沉默型最低。不同基因型植株的 H2 O2和 MDA 的含量正好相反,说明 AtHsfA1a 通过保护细胞膜而减小细胞所受氧化伤害来提高抗旱能力。 相似文献
179.
1990年,澳大利亚联邦政府把残疾人视为高等教育中的弱势群体,自此澳大利亚联邦政府开始制定政策来保障残疾人的高等教育入学机会。《澳大利亚残疾人支持计划》的目的就是保障该类群体的入学机会。本文通过分析的政策文本,政策实施,政策理念和特点来分析《高等教育残疾人》政策。 相似文献
180.
Daniel Tan Lei Shek Florence Ka Yu Wu Wen Yu Chai 《Assessment & Evaluation in Higher Education》2015,40(8):1017-1031
Under the new four-year undergraduate programme, a general education framework titled ‘General University Requirements’ (GUR) has been developed and implemented since 2012/2013 at Hong Kong Polytechnic University (PolyU). To evaluate the implementation and effectiveness of the GUR in its first year, focus group interviews with students were carried out. In 13 focus groups, 62 first-year students from eight faculties/schools who took GUR subjects in 2012/2013 academic year were interviewed by trained researchers. Results showed that students generally had good perceptions of the GUR in terms of the subject content, teaching methods and the overall effectiveness of the subjects. The interactive teaching and learning initiatives adopted in GUR subjects were particularly appreciated, although students expressed minor concerns on the administration of GUR subjects. The results suggest that the first-year implementation of the GUR at PolyU was generally smooth and successful from the perspectives of the students. 相似文献