Microfluidic droplet encapsulation of highly motile single zoospores for phenotypic screening of an antioomycete chemical

Highly motile Phytophthora sojae (P. sojae) zoospores of an oomycete plant pathogen and antioomycete candidate chemicals were encapsulated into microdroplets. Random fast self-motion of P. sojae zoospores was overcome by choosing an appropriate flow rate for a zoospore suspension. To influence stochastic loading of zoospores into a microfluidic channel, a zoospore suspension was directly preloaded into a microtubing with a largely reduced inner diameter. A relatively high single zoospore encapsulation rate of 60.5% was achieved on a most trivial T-junction droplet generator platform, without involving any specially designed channel geometry. We speculated that spatial reduction in the diameter direction of microtubing added a degree of zoospore ordering in the longitudinal direction of microtubing and thus influenced positively to change the inherent limitation of stochastic encapsulation of zoospores. Comparative phenotypic study of a plant oomycete pathogen at a single zoospore level had not been achieved earlier. Phenotypic changes of zoospores responding to various chemical concentration conditions were measured in multiple droplets in parallel, providing a reliable data set and thus an improved statistic at a low chemical consumption. Since each droplet compartment contained a single zoospore, we were able to track the germinating history of individual zoospores without being interfered by other germinating zoospores, achieving a high spatial resolution. By adapting some existing droplet immobilization and concentration gradient generation techniques, the droplet approach could potentially lead to a medium-to-high throughput, reliable screening assay for chemicals against many other highly motile zoospores of pathogens.     

高校创业教育社会支持体系构建

高校创业教育有着显著的社会实践性,对社会资源的依赖性强,提高高校创业教育质量,破解大学生低创业率与低成功率"两低"问题需要广泛的社会支持.对创业教育认识不够、内生动力不足以及互动机制不健全等原因,导致高校创业教育社会支持的不足.实现优势互补、相互配合、资源共享,增强高校创业教育社会支持效果,需要构建构成要素明确、角色定位清晰、保障机制健全的"六方协同"的高校创业教育社会支持体系.     

烟草商业企业财务共享服务中心建设探究与实践

近年来,随着财务数字化和财务职能转型,烟草行业财务智能化建设不断深入,多数商业企业探索建设FSSC(财务共享服务中心).FSSC作为一种起源于20世纪80年代的先进管理模式,在实践中有着成熟的应用经验,但是烟草行业作为特殊行业,兼具企业和行政双重职能,在建设过程中存在较多特殊性,无法照搬照抄.从烟草行业智能财务建设背景出发,阐明建设财务共享服务中心的必要性,梳理实践中遇到的困境,提出"小步快走,分步实施"的总体思路,从顶层设计、配套制度、数据挖掘、应用导向等方面提出针对性措施.     

加强新建地方本科院校思想政治理论课网络实践教学资源建设和利用的探索

实践教学是实现高校思想政治理论课教育教学目的的必要环节.当前信息网络化形势下新建地方本科院校应建设并充分利用网络平台开展思想政治理论课实践教学,这对于提高大学生对思想政治理论课的学习兴趣和增强其教学实效性有着重要意义.     

现代汉语双宾句分类研究综述

双宾句是现代汉语中比较有特色的一种句式,长久以来一直受到许多语法学家的重视。对双宾句的研究不仅有助于人们了解现代汉语语法体系,而且有助于人们解释更多的现代汉语语法现象。随着研究的深入,双宾句分类研究也取得了丰硕成果,但同时也存在一些问题,需要学者们去克服,以提升近代汉语研究的水平和层次。     

从“有机运动”到“有机规制”:马克思主义新闻观视域下的新闻自由

互联网通信技术及其应用的迭代发展,使得自媒体语境下的新闻传播活动有了更多元的生存空间和表现形态,也给新闻自由带来了更多矛盾问题.文章基于既有的文献和当下的新闻实践,在马克思主义新闻观的范式下分析社会主义新闻自由的本质及其发展现状,并指出中国当下自媒体新闻的“非有机运动”问题.泛滥的自由会导致人民的自由本身受损,文章继承和发展了马克思主义的“有机运动”理论,针对性地提出“有机规制”这一治理路径,保障社会主义的新闻自由.     

刘咸炘《吕氏春秋》研究述要

刘咸炘关于《吕氏春秋》的研究成果极为丰富,集中反映在所著《吕氏春秋发微》一书中。该书论列各篇,胜义纷呈,然而迄今尚未引起学界关注。本文将其研究成果归结为五个方面：辨杂家、论体式、明主旨、撮篇义、贯全书,并结合相关资料,进行了论述。期于引起学界注意,以便加强进一步的研究。     

我国城镇化发展及其实现路径

马克思的城市化理论具有较强的生命力,对于我国城镇化有重要的分析与指导作用。自新中国成立至今,我国城镇化已经历了四个阶段,当前正处于快速发展的第五个阶段。回顾城镇化发展历程,发现我国城镇化的实现路径主要有政策导向与市场推动、农村剩余劳动力转移为主要人口来源、人口的增加与土地利用的扩大、城乡差距扩大以及城市与城市差距扩大等方面。为了实现城镇化的可持续,必须走新型城镇化道路,即以人的城镇化为根本、以良好的生态为基础、以提高城镇承载量为手段,以及以城乡融合为目标。     

Does evaluating peer assessment accuracy and taking it into account in calculating assessor’s final score enhance online peer assessment quality?

Education and Information Technologies - Peer assessment has an important role in teaching and learning nowadays. However, the existing techniques tend to be limited due to a lack of a suitable...     

The discourse of design: Patterns of TPACK Contribution during pre-service teacher learning design conversations

Education and Information Technologies - Analysing pre-service teachers’ learning design conversations in relation to Technological Pedagogical and Content Knowledge (TPACK) framework to...