DNA capture-probe based separation of double-stranded polymerase chain reaction amplification products in poly(dimethylsiloxane) microfluidic channels

Herein, we describe the development of a novel primer system that allows for the capture of double-stranded polymerase chain reaction (PCR) amplification products onto a microfluidic channel without any preliminary purification stages. We show that specially designed PCR primers consisting of the main primer sequence and an additional “tag sequence” linked through a poly(ethylene glycol) molecule can be used to generate ds-PCR amplification products tailed with ss-oligonucleotides of two forensically relevant genes (amelogenin and human c-fms (macrophage colony-stimulating factor) proto-oncogene for the CSF-1 receptor (CSF1PO). Furthermore, with a view to enriching and eluting the ds-PCR products of amplification on a capillary electrophoretic-based microfluidic device we describe the capture of the target ds-PCR products onto poly(dimethylsiloxane) microchannels modified with ss-oligonucleotide capture probes.     

Comparisons of achievement, effort, and self-perceptions among students with learning disabilities and their peers from different achievement groups

This study compared the social-emotional implications of academic achievement for students with and without learning disabilities (LD) and identified predictors of effort investment. Students with LD showed lower levels of achievement, effort investment, academic self-efficacy, sense of coherence, positive mood, and hope, and higher levels of loneliness and negative mood. When compared to peers without LD (n = 447) at four different academic achievement levels, students with LD showed higher achievement than the low-average group, but their social-emotional profiles were similar to the low and low-average groups. Hierarchical multiple regression analysis showed that achievement, academic self-efficacy, negative mood, and hope predicted effort investment for students with LD. These results demonstrated the importance of hope in understanding the functioning of students with LD.     

Relationships among childhood maltreatment, PTSD, and health in female veterans in primary care

OBJECTIVE: Women with histories of childhood maltreatment (CM) have higher rates of physical health problems and greater medical utilization compared to women without abuse histories. This study examined whether current post-traumatic stress disorder (PTSD) symptoms mediate the relationship between CM and indicators of physical health and medical utilization in female veterans. METHOD: Respondents were 221 female veterans (56% of the potential sample), who received medical care from the San Diego VA Healthcare System during a 12-month period. Respondents provided self-report information about CM, PTSD symptoms, use of pain medication, and physical symptoms and functioning. Additional information about medical utilization was extracted from respondents' medical charts. Regression-based models were conducted to test whether PTSD symptoms mediate the relationships between CM and physical symptoms and between CM and medical utilization. RESULTS: Emotional abuse was associated with poorer role-physical functioning, increased bodily pain and greater odds of using pain medication in the past 6 months. Physical abuse was associated with poorer general health. Contrary to prediction, emotional neglect was associated with better role-physical functioning, and CM was not associated with increased healthcare utilization. PTSD was shown to mediate the relationship between emotional and physical abuse and health outcomes. CONCLUSIONS: PTSD, or psychopathology more generally, appears to be an important factor in the negative health impact of CM. Given that several empirically supported interventions are available for PTSD, there may be physical health benefits in early identification and treatment of psychopathology related to CM.     

British Colonial Administrations’ registry systems: a comparative study of Northern Rhodesia and Nyasaland

Recordkeeping in twentieth century colonial states has been ignored by scholars, or at best regarded as the result of a monolithic process of migration of metropolitan practices. Registries provided the principal organ of recordkeeping in British government systems of administration. This comparative study of the registries in the neighbouring territories of Northern Rhodesia (now Zambia) and Nyasaland (now Malawi) reveals the true extent of metropolitan British influence, exposing an absence of centralised advice, and a consequent dependence upon individual innovation. This engendered divergent and sometimes weak recordkeeping regimes. The effects of this included the diminution of fiduciary responsibility and administrative accountability.     

Imperatives for regular collection inventories: A New Zealand university library's experience

This paper describes three imperatives for collection inventory work at the University of Canterbury (UC) Library. Learning experiences from UC Library's first full inventory in 2005 were used to develop a regular and sustainable method of conducting future inventories. The first need for inventory arose as a result of an organized crime ring that targeted valuable books in libraries throughout New Zealand. Findings from library customer surveys in 2003 and 2005 gave rise to a second imperative which was to take prompt, proactive action regarding items missing from the shelves. As UC Library begins moving items to offsite storage, a third imperative arose for correctly accounting for what UC Library actually holds. As pressure comes on staffing resources this additional and significant collection management task has had to be achieved without extra staff. Good communication, clarity of responsibilities and breaking down the work into manageable amounts all became vital to the overall success of managing inventory work.