Size and shape based chromosome separation in the inertial focusing device

In this paper, we use a spiral channel inertial focusing device for isolation and purification of chromosomes, which are highly asymmetric. The method developed is proposed as a sample preparation process for transchromosomic research. The proposed microfluidics-based chromosome separation approach enables rapid, label-free isolation of bioactive chromosomes and is compatible with chromosome buffer. As part of this work, particle force analysis during the separation process is performed utilizing mathematic models to estimate the expected behavior of chromosomes in the channel and the model validated with experiments employing fluorescent beads. The chromosome sample is further divided into subtypes utilizing fluorescent activated cell sorting , including small condensed chromosomes, single chromosomes, and groups of two chromosomes (four sister chromatids). The separation of chromosome subtypes is realized based on their shape differences in the spiral channel device under high flow rate conditions. When chromosomes become aligned in the shear flow, the balance between the inertial focusing force and the Dean flow drag force is determined by the chromosome projection area and aspect ratio, or shape difference, leading to different focusing locations in the channel. The achieved results indicate a new separation regime in inertial microfluidics that can be used for the separation of non-spherical particles based on particle aspect ratios, which could potentially be applied in fields such as bacteria subtype separation and chromosome karyotyping.     

Classing schools

School prospectuses and promotional videos appeal to parents by presenting idealised images of the education a school provides. These educational idealisations visually realise the form of discipline a school is expected to provide, depending on the social habitus of the parents. This paper presents a content analysis of the images used in 33 sets of marketing materials from a maximally diverse sample of schools from the state of Victoria. These images are interpreted using the lenses of Bernstein's control and Bourdieu's habitus and cultural capital. The promotional images are found to vary systematically in terms of content and form depending on the perceived social class of the students which the schools attract.     

Calculating student aspiration: Bourdieu,spatiality and the politics of recognition

This paper reports on a recent study of aspirations for higher education by secondary school students from disadvantaged backgrounds in regional Australia. At the same time, it goes in search of explanations that transcend a Bourdieuian account of aspirations as produced by and reproductive of cultural histories and dominance, given the apparent inadequacy of these accounts in redressing disadvantage. To this end the authors distinguish between historicising and spatialising aspirations, taking up Appadurai’s notion of navigational capacity as a way of advancing greater agency for disadvantaged groups. Data from the research inform the analysis, including the mediation of students’ desired futures by their perception of what is possible given their differentiated locations and access to resources. It is concluded that while this spatial turn in theorising aspiration has potential for changing the terms of recognition internal to disadvantaged communities, there remain structural limits on change ‘from below’.     

Addressing Challenges to Public Understanding of Science: Epistemic Cognition,Motivated Reasoning,and Conceptual Change

Science is of critical importance to daily life in a knowledge society and has a significant influence on many everyday decisions. As scientific problems increase in their number and complexity, so do the challenges facing the public in understanding these issues. Our objective is to focus on 3 of those challenges: the challenge of reasoning about knowledge and the processes of knowing, the challenge of overcoming biases in that reasoning, and the challenge of overcoming misconceptions. We propose that research in epistemic cognition, motivated reasoning, and conceptual change can help to identify, understand, and address these obstacles for public understanding of science. We explain the contributions of each of these areas in providing insights into the public's understandings and misunderstandings about knowledge, the nature of science, and the content of science. We close with educational recommendations for promoting scientific literacy.     

Improving and Maintaining Torque Accuracy in Aerospace Manufacturing: A Case Study

Needs assessment is a systematic and systemic process intended to identify and define gaps between current performance and desired performance. Needs assessments are most beneficial if they address issues that affect successful accomplishment of operational or tactical processes as well as outcomes that allow an organization to meet its overall strategic goals. A team of graduate students took on a needs assessment project to ascertain the causes of declining accuracy in a key task in an aerospace manufacturing process. The project encountered numerous barriers within the client organization, but the team systematically applied human performance technology methods in multiple stages to gather and analyze the available data. The end result was a set of identified root causes and recommended interventions focused on improving performance.