几种药用植物基因组DNA提取方法研究

采用改良2×CTAB法和改良SDS法提取富含多糖和次生代谢物质的几种药用植物不同器官基因组DNA,并用紫外分光光度计分析,凝胶电泳和PCR扩增进行鉴定。结果表明:改良2×CTAB法和改良SDS法均能有效去除不同药用植物材料中的蛋白质、多糖、酚类及其他次生代谢物质,获得较高质量基因组DNA。但与改良SDS法相比,改良2×CTAB法提取的基因组DNA质量更好。     

Optimal ϵ -stealthy attack in cyber-physical systems

This paper is concerned with the optimal stealthy attack problems of cyber-physical system, which is represented as discrete-time linear systems. It is considered that a deceptive attack assumed to be able to hijack and modify the nominal control signals from the controller-actuator channel with the metric of ? -stealthiness. Different from the existing literatures, some optimal attack strategies with objectives such that maintaining a fixed level of stealthiness and achieving the maximized performance degradation are designed under some constraints. It is further derived that the results of ours are more relaxed than some methods proposed before. Finally, some numerical simulations are given to illustrate the validity of the theoretical results.     

Electrophoretic separation of neurotransmitters on a polystyrene nano-sphere∕polystyrene sulphonate coated poly(dimethylsiloxane) microchannel

In this paper, a poly(dimethylsiloxane) microchip with amperometric detector was developed for the electrophoretic separation and determination of neurotransmitters. For increasing the separation efficiency, the microchannel is modified by polystyrene sulphonate∕polystyrene nano-sphere self-assembly coating. A stable electro-osmotic flow (EOF) and higher separation efficiency are obtained in proposed modified microchannel. Under optimized conditions, dopamine, epinephrine, catechol, and serotonin are acceptably baseline separated in this 3.5 cm length separation channel with the theoretical plate number from 4.6?×?10(4) to 2.1?×?10(5) per meter and resolution from 1.29 to 12.5. The practicability of proposed microchip is validated by the recovery test with cerebrospinal fluid as real sample which resulted from 91.7% to 106.5%.     

Electrophoretic separation of neurotransmitters on a polystyrene nano-sphere�Mpolystyrene sulphonate coated poly(dimethylsiloxane) microchannel

In this paper, a poly(dimethylsiloxane) microchip with amperometric detector was developed for the electrophoretic separation and determination of neurotransmitters. For increasing the separation efficiency, the microchannel is modified by polystyrene sulphonate∕polystyrene nano-sphere self-assembly coating. A stable electro-osmotic flow (EOF) and higher separation efficiency are obtained in proposed modified microchannel. Under optimized conditions, dopamine, epinephrine, catechol, and serotonin are acceptably baseline separated in this 3.5 cm length separation channel with the theoretical plate number from 4.6 × 10⁴ to 2.1 × 10⁵ per meter and resolution from 1.29 to 12.5. The practicability of proposed microchip is validated by the recovery test with cerebrospinal fluid as real sample which resulted from 91.7% to 106.5%.     

Beijing's West District Library Enhances International Cooperation

<正>The West District Library of Beijing is China’s first county/district-level public library granted with the member status of the International Federation of ...     

高职制药校内外生产性实训基地功能定位探析

针对高职制药类专业生产性实训基地建设水平低的现状,分析制药校内外生产性实训基地发展缓慢的原因,提出制药生产性实训基地建设首先要解决功能定位问题;制药类专业生产性实训基地应以药物研发、社会服务为方向,将“药品生产”型实训基地通过深化校企合作建在校外企业。     

任务型教学法在高级英语教学中的应用

以学生为中心的任务型教学法在独立学院高校英语专业教学中的应用日渐普遍。文章以高级英语教学实践为例,重点分析根据任务型教学法的原则和结构模式,合理设计独立学院高级英语课堂教学任务。     

江苏省立国学图书馆与中国文化传承研究

近代西方欧美文化输入中国后,绵延数千年的传统文化面临空前的危机,中国文化传承成为时人思考的深切主题。本文重点探讨江苏省立国学图书馆民国时期古籍收藏及出版、木刻版片保存等方面的业绩,以阐扬其传承中国文化的卓越贡献。     

“新工科”与高职院校产教融合的机理耦合、现实困境与优化路径

随着教育部首批“新工科”项目的正式公布,推动落实高等工程教育改革的脚步进一步加快。高职院校作为培育高等工程技术人才的重要机构,同样承担着推动高职工程教育改革的使命。文章以高校“新工科”建设为切入点,从理论、实践、功能价值三方面系统阐述了“新工科”建设与高职产教融合的内在耦合性,并针对产教融合背景下高职院校“新工科”建设面临的学科壁垒、制度僵化、资源劣势等现实困境,提出基于产教融合命运共同体的高职“新工科”建设优化路径,以期为高职工程教育改革加大助力,同时不断推动我国高职产教融合的常态化、规范化、制度化发展。     

后控规范的计算机处理

分析了建立情报检索系统时受控主题标引的不足之处：标引效率低、语义网得不到扩充、不一致的组配标引。提出了以后控规范为基础的标引体系。为使后控规范能方便地由计算机实现，研究了利用相似性匹配技术找出语义上有一定联系的术语，并由计算机半自动地建立用、代、属、分、参等语义关系的方法。