Haemoglobin A1c-based screening for prediabetes and diabetes mellitus: a multi-center study in Croatian adult population

IntroductionBased on the hypothesis that there is a substantial rate of adults with prediabetes and undiagnosed diabetes mellitus (DM), our aim was to perform haemoglobin A1c (HbA_1c)-based screening in a cohort of Croatian adults and estimate the prevalence of prediabetes and undiagnosed DM according to American Diabetes Association criteria.Materials and methodsThis multi-center, cross-sectional study performed in six Croatian hospitals included 5527 patients aged 40 to 70 years admitted to the Emergency Department or undergoing a primary care check-up. Haemoglobin A1c was measured from leftover whole blood samples using the enzymatic method on either Alinity c or Architect c-series analyser (Abbott Laboratories, Chicago, USA). Haemoglobin A1c between 39-47 mmol/mol was classified as prediabetes, while ≥ 48 mmol/mol as undiagnosed DM.ResultsAfter exclusion of 435 patients with known DM, the final cohort included 5092 patients (median age 57; 56% males). A total of 882 (17.3%) patients had HbA_1c values between 39 and 47 mmol/mol. There were 214 (4.2%) patients with HbA_1c ≥ 48 mmol/mol. Prediabetes prevalence ranged from 14.2% to 20.5%, while undiagnosed DM from 3.3% to 7.3%, with statistically significant differences among settings (P < 0.001). Age-stratified analysis showed that prediabetes and undiagnosed DM prevalence increase with age (P < 0.001), being 25.4% and 5.8%, respectively, in patients aged 60 to 70 years.ConclusionUnderlying impairment of glucose metabolism was identified in about one in five adults, with significant number of patients with already overt DM. These results should serve as a starting point for further steps directed towards promotion of preventive measures for DM in Croatia.     

Comparison of diagnostic accuracy for eight SARS-CoV-2 serological assays

IntroductionSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) serological tests have been suggested as an additional diagnostic tool in highly suspected cases with a negative molecular test and determination of seroprevalence in population. We compared the diagnostic performance of eight commercial serological assays for IgA, IgM, and IgG antibodies to the SARS-CoV-2 virus.Materials and methodsThe comparison study was performed on a total of 76 serum samples: 30 SARS-CoV-2 polymerase chain reaction (PCR)-negative and 46 SARS-CoV-2 PCR-positive patients with asymptomatic to severe disease and symptoms duration from 3-30 days. The study included: three rapid lateral flow immunochromatographic assays (LFIC), two enzyme-linked immunosorbent assays (ELISA), and three chemiluminescence immunoassays (CLIA).ResultsAgreement between IgM assays were minimal to moderate (kappa 0.26 to 0.63) and for IgG moderate to excellent (kappa 0.72 to 0.92). Sensitivities improved with > 10 days of symptoms and were: 30% to 89% for IgM; 89% to 100% for IgG; 96% for IgA; 100% for IgA/IgM combination; 96% for total antibodies. Overall specificities were: 90% to 100% for IgM; 85% to 100% for IgG; 90% for IgA; 70% for IgA/IgM combination; 100% for total antibodies. Diagnostic accuracy for IgG ELISA and CIA assays were excellent (AUC ≥ 0.90), without significant difference. IgA showed significantly better diagnostic accuracy than IgM (P < 0.001).ConclusionThere is high variability between IgM assays independently of the assay format, while IgG assays showed moderate to perfect agreement. The appropriate time for testing is crucial for the proper immunity investigation.     

The effects of two different correction strategies on the snatch technique in weightlifting

Improving motor skills represents one of the major issues in motor control and motor learning literature. The aim of this study was to investigate which of two strategies, method of amplification of error (MAE) or direct instruction (DI), would be more beneficial for error correction of the snatch technique. Thirty well-trained male weightlifters were randomly assigned to one of three training conditions (MAE, DI and Control). The experiment took place in only one practice session in which each lifter performed 3 pretraining trials, 8 training intervention trials, and 3 post-training trials, and a retention test session after 1 week. An optoelectronic motion capture system was used to measure the kinematic parameters of the weightlifting performance. After the training intervention, data showed that the MAE group revealed a greater improvement in several kinematic parameters when compared to the DI and Control groups, and the benefits derived from its application were still present 1 week later in the retention test. Nevertheless, the findings of the present study should be interpreted with caution due to the relatively small sample size; further research will also be necessary to evaluate the effects of MAE with different ability levels and other sport skills. The present findings could have practical implications for sport psychology and physical education because while practice is obviously necessary for improving learning, the efficacy of the learning process is essential in enhancing learners’ motivation and sport enjoyment.     

National Guidelines for the Performance of the Sweat Test in Diagnosis of Cystic Fibrosis on behalf of the Croatian Society of Medical Biochemistry and Laboratory Medicine and the Cystic Fibrosis Centre - Paediatrics and adults,University Hospital Centre Zagreb

The sweat test (ST) is a cornerstone in the diagnosis of cystic fibrosis (CF), together with newborn screening and genetic testing. However, the performance of the ST can depend on the operator’s skill, so several international guidelines have been published to standardise the ST, but inconsistencies remain. The joint Working Group for ST Standardisation (WG STS) of the Croatian Society of Medical Biochemistry and Laboratory Medicine, in association with cistic fybrosis health professional and the Cistic Fibrosis Centre for Paediatrics and Adults, have issued National Guidelines for the Performance of the Sweat Test in order to ensure consistency in ST performance and accuracy of reported results. Many of the standards were taken from the 2nd Edition of the UK Guidelines for Performance of the ST for the Diagnosis of CF, while others were taken from independent consensus statements from the WG STS based on local ST equipment and practices. The standards cover every step of the ST, from the indications for testing to reporting of results and their interpretation, including the analytical phase and quality control. In addition, National Guidelines include appendices with practical examples in order to aid implementation of the recommendations in routine practice.     

Functioning of the attentional networks at rest vs. during acute bouts of aerobic exercise

The present study explored the effects of three different activity conditions on three attentional functions: alerting, orienting, and executive control. A group of highly experienced cyclists performed the Attention Network Test-Interactions (Callejas, Lupiá?ez, & Tudela, 2004) at rest, during moderate aerobic exercise, and during intense aerobic exercise. Results indicated that aerobic exercise accelerated reaction time and reduced the alerting effect compared with the rest condition. However, aerobic exercise did not modulate the functioning of either the orienting or the executive control attentional networks. No differences in reaction time or attentional functioning were observed between the two aerobic exercise workloads. The present results suggest that moderate aerobic exercise modulates the functioning of phasic alertness by increasing the general state of tonic vigilance.     

Applicability assessment of Semantic Web technologies

The Semantic Web is one of the fastest developing fields within the Information and Communication Technology sector and, as such, under constant examination by scientists and IT professionals. This article aims to provide a better understanding of the applicability of Semantic Web tools and technologies in practice. This aim will be achieved by surveying the recommended and emerging W3C standards, presenting an overview of the state-of-the-art in the Semantic Web research in the European Union, analysing the W3C collection of Case studies and Use Cases, and discussing the extent of adoption of Semantic Web technologies. The overall technology maturity level assessment has shown that Semantic Web technologies are finding their ways into real-world applications, and that, rather than being merely a fashionable research issue, the Semantic Web, slowly but surely, becomes our reality.     

Moderation and consistency of teacher judgement: Teachers’ views

Major curriculum and assessment reforms in Australia have generated research interest in issues related to standards, teacher judgement and moderation. This article is based on one related inquiry of a large‐scale Australian Research Council Linkage project conducted in Queensland. This qualitative study analysed interview data to identify teachers’ views on standards and moderation as a means to achieving consistency of teacher judgement. A complementary aspect of the research involved a blind review that was conducted to determine the degree of teacher consistency without the experience of moderation. Empirical evidence was gained that most teachers, of the total interviewed, articulated a positive attitude towards the use of standards in moderation and perceived that this process produces consistency in teachers’ judgements. Context was identified as an important influential factor in teachers’ judgements and it was concluded that teachers’ assessment beliefs, attitudes and practices impact on their perceptions of the value of moderation practice and the extent to which consistency can be achieved.     

Apples and oranges: Educational enrollment and attainment across countries in Latin America and the Caribbean

This paper uses household survey data to rank LAC countries’ performance in two areas: (i) getting children into school on time and keeping them there, and (ii) turning their contact with the educational system into years of schooling. It presents multiple rankings because most countries’ performance is not uniform across these dimensions. For instance, the Dominican Republic performs almost as well as the richest countries when it comes to keeping children in school, but as badly as the poorest few in terms of turning attendance into years of schooling. Further, the rankings, which are based on conventional and new measures of educational systems’ performance, are occasionally quite different from those obtained using more widely available administrative data.     

Self-regulated learning and the role of ePortfolios in business studies

Students’ work in ePortfolios was assessed through a case study supported by observation techniques and eQuestionnaires to gather data from a sample of eighty students over a period of 4 years (20 students per academic year). The main purpose of the study was to explore whether ePortfolios can be used efficiently to support the learning process of postgraduate business students taking a course where finance modules were a major component. The use, role and learning value of integrating ePortfolios into the learning process in a higher education institution was analysed over the period of study. The empirical findings suggest that ePortfolios could be used to facilitate and enhance students’ self-regulated learning. Additionally, the role of the instructor was found to be fundamental during early stages of the learning process to guide and coach students on how to use the tool. This role diminished as students became familiar with the course requirements. Overall, students judged the ePortfolio as being positive to their education, noting a significant improvement in their learning experience when compared to a traditional learning approach. The evidence suggests that ePortfolios could be used to support technical and complex modules in a controlled environment. At the same time, ePortfolios were found to be flexible enough to give students creative space by helping them to integrate their own ideas and views through their learning process. Students were also able to offer their instructors feedback on their learning achievements.     

Predictors of reading and comprehension abilities in bilingual and monolingual children: a longitudinal study on a transparent language

Many studies have shown that learning to read in a second language (L2) is similar, in many ways, to learning to read in a first language (L1). Nevertheless, reading development also relies upon oral language proficiency and is greatly influenced by orthographic consistency. This longitudinal study aimed to analyze the role of linguistic predictors (phonological awareness, letter knowledge, pseudoword repetition, morphosyntactic comprehension, lexical knowledge and rapid naming) in reading outcomes (fluency, accuracy and comprehension) in a group of bilingual children (n = 30) reading Italian as an L2, compared to a group of monolingual children (n = 56). We ran a multi-group structural equation model. Our findings showed that rapid automatized naming was a significant predictor of reading speed in both groups. However, the study revealed different patterns of predictors for reading accuracy, predictors for monolinguals being LK, phonological awareness and lexical knowledge, while pseudoword repetition was a predictor for bilinguals. Morphosyntactic comprehension was the most significant predictor of comprehension skills in bilingual children. Implications for clinical and educational settings are discussed.