生物安全实验室建设与运行管理实践

生物安全实验室为传染病的防治研究提供了必需的公共研究平台。中山大学生物安全实验室结合自身的实际情况,建立了严格的管理制度及各项措施,使实验室的运行管理更规范化、标准化,并实现了实验室资源共享平台的网络预约,确保了实验室设施和仪器设备的有效共享及安全、高效的运行。     

基于行动导向的《信息沟通辅助技术》课程设计与实施

目的:应用行动导向方法进行《信息沟通辅助技术》课程设计与实施,探讨行动导向教学法在教学中的应用效果。方法:采用访谈法、实地研究法等进行课程涉及的岗位分析、典型职业任务归纳和学习情境的设计。使用行动研究法不断改进行动导向的教学实施。结果:根据教学效果的观察以及对学生、实习单位负责人的访谈结果表明,行动导向教学有利于知识的理解掌握和应用;同时培养了学生的方法能力与社会能力。结论:行动导向教学法的课程改革已经在教学一线凸显实效,但仍需要不断探索和实践。     

外商在华投资现状研究与分析

文章的主题是"外商在华投资"。主要包括四个部分:首先分析外商在华投资的现状,提出了外资进入中国的主要原因,分析外商投资存在的问题以及对中国经济环境造成的影响,最后提出解决方法以及如何正确应对这些问题。     

Teachers’ and Students’ Conceptions of Good Science Teaching

Capitalizing on the comments made by teachers on videos of exemplary science teaching, a video-based survey instrument on the topic of ‘Density’ was developed and used to investigate the conceptions of good science teaching held by 110 teachers and 4,024 year 7 students in Hong Kong. Six dimensions of good science teaching are identified from the 55-item questionnaire, namely, ‘focussing on science learning’, ‘facilitating students’ understanding’, ‘encouraging students’ involvement’, ‘creating conducive environment’, ‘encouraging active experimentation’ and ‘preparing students for exam (PSE)’. Significant gaps between teachers’ and students’ conceptions on certain dimensions have been revealed. The inconsistency on the dimension ‘PSE’ is particularly evident and possible reasons for the phenomenon are suggested. This study raises the important questions of how the gap can be addressed, and who is to change in order to close the gaps. Answers to these questions have huge implications for teacher education and teacher professional development.     

Exploring foreign undergraduate students’ experiences of university

Although international students are an important source of income to universities in the UK, the emotional impact of their experiences may be ignored and unacknowledged. This study explored the personal experiences of international students studying for an undergraduate degree in the UK. Semi-structured interviews with five participants were analysed using interpretative phenomenological analysis, which aimed to capture the personal perceptions of these participants. Two analytical themes are presented: “adaptation and identity”, which reflects the difficulties in acculturation, adaptation and development of identity in a foreign country; and “us and them”, which pays close attention to the perception of disconnectedness, unfairness and exclusion encountered in trying to integrate into a different way of life. Findings are discussed in relation to the impact that participants’ experiences have had on their self-worth, confidence and ability to engage with domestic individuals.     

构建基于大数据的智能高校信息化管理服务系统

在大数据的背景下,结合对高校信息化中服务发展的需求,提出建立智能高校信息化管理服务系统的思路。依据大数据的基本思想,首先提出高校大数据的概念并分析其基本特征。对智能高校信息化服务具体体现方式进行研究,详细阐述建设智能高校信息化管理服务系统的原则和构架设计的逻辑框架,进一步探讨在实践中ETL、数据仓库建模等关键技术,以实例说明智能信息化管理中的数据应用服务和决策支持,为全面建立智能化的高校信息化管理服务系统提供探索和实践基础。     

Confirmatory Factor Analysis of the Test of Gross Motor Development-2

The purpose of this study was to examine the underlying structure of the second edition of the Test of Gross Motor Development-2 (Ulrich, 2000 Ulrich, D. A. 2000. Test of gross motor development: Examiner's manual, 2nd, Austin, TX: Pro-.  [Google Scholar]) as applied to Chinese children. The Test of Gross Motor Development-2 was administered to 626 Hong Kong Chinese children. The outlier test with standard scoring was utilized. After data screening, a total of 614 cases (N = 614) were used for further analysis. The two-factor structure of the Test of Gross Motor Development-2 was tested using confirmatory factor analysis with maximum likelihood estimation to compute parameter estimates and to select the appropriate item for each factor. The goodness-of-fit indices supported that the model was tenable (goodness-of-fit index = .95, root mean square error of approximation = .06, standardized root mean square residual = .04, comparative-fit index = .97). The findings of this study suggested that the two-factor structure proposed by Ulrich (2000 Ulrich, D. A. 2000. Test of gross motor development: Examiner's manual, 2nd, Austin, TX: Pro-.  [Google Scholar]) fit the data of Hong Kong Chinese children.     

太极拳入门功法创编标准的研究

随着人们生活质量的不断提高,太极拳的健身价值在世界舞台上也越来越受人关注。与此同时,由太极拳衍生而来的入门功法也是层出不穷。但是,太极拳的入门功法并不能随意编创,需要一个标准对其约束,力求使功法具备科学性、规范性、适用性等。本研究主要采用文献资料法,以太极拳拳理、技法特征为理论立足点,为太极拳入门功法制定了较为妥善的标准,为太极拳入门功法的编创指明了方向。     

A negative-pressure-driven microfluidic chip for the rapid detection of a bladder cancer biomarker in urine using bead-based enzyme-linked immunosorbent assay

This paper describes an integrated microfluidic chip that is capable of rapidly and quantitatively measuring the concentration of a bladder cancer biomarker, apolipoprotein A1, in urine samples. All of the microfluidic components, including the fluid transport system, the micro-valve, and the micro-mixer, were driven by negative pressure, which simplifies the use of the chip and facilitates commercialization. Magnetic beads were used as a solid support for the primary antibody, which captured apolipoprotein A1 in patients'' urine. Because of the three-dimensional structure of the magnetic beads, the concentration range of the target that could be detected was as high as 2000 ng ml⁻¹. Because this concentration is 100 times higher than that quantifiable using a 96-well plate with the same enzyme-linked immunosorbent assay (ELISA) kit, the dilution of the patient''s urine can be avoided or greatly reduced. The limit of detection was determined to be approximately 10 ng ml⁻¹, which is lower than the cutoff value for diagnosing bladder cancer (11.16 ng ml⁻¹). When the values measured using the microfluidic chip were compared with those measured using conventional ELISA using a 96-well plate for five patients, the deviations were 0.9%, 6.8%, 9.4%, 1.8%, and 5.8%. The entire measurement time is 6-fold faster than that of conventional ELISA. This microfluidic device shows significant potential for point-of-care applications.     

中职学校会计专业技能训练之我见

会计具有专业性、实用性很强的特点,教师在会计教学过程中,不仅要向学生全面系统地传授基本会计理论和会计方法,而且更注重培养应用会计理论和方法、解决会计实践问题的能力。