Separation and enrichment of sodium-motile bacteria using cost-effective microfluidics

Many motile bacteria are propelled by the rotation of flagellar filaments. This rotation is driven by a membrane protein known as the stator-complex, which drives the rotor of the bacterial flagellar motor. Torque generation is powered in most cases by proton transit through membrane protein complexes known as stators, with the next most common ionic power source being sodium. Sodium-powered stators can be studied through the use of synthetic chimeric stators that combine parts of sodium- and proton-powered stator proteins. The most well studied example is the use of the sodium-powered PomA-PotB chimeric stator unit in the naturally proton-powered Escherichia coli. Here we designed a fluidics system at low cost for rapid prototyping to separate motile and non-motile populations of bacteria while varying the ionic composition of the media and thus the sodium-motive force available to drive this chimeric flagellar motor. We measured separation efficiencies at varying ionic concentrations and confirmed using fluorescence that our device delivered eightfold enrichment of the motile proportion of a mixed population. Furthermore, our results showed that we could select bacteria from reservoirs where sodium was not initially present. Overall, this technique can be used to implement the selection of highly motile fractions from mixed liquid cultures, with applications in directed evolution to investigate the adaptation of motility in bacterial ecosystems.     

A Symbiosis of Access: Proliferating STEM PhD Training in the U.S. from 1920–2010

Minerva - Over the course of the 20th century, unprecedented growth in scientific discovery was fueled by broad growth in the number of university-based scientists. During this period the American...     

Comparison of methods for determining power generated on a rope-braked cycle ergometer during low-intensity exercise

This study compares the instrumentation and analysis techniques used when determining the power expended pedalling a rope-braked ergometer manufactured by Monark (Sweden) during a low intensity test. Power values were generated by eight subjects. The instrumentation consisted of load cells to measure the rope brake forces, a tachometer to measure the flywheel velocity and instrumented pedal cranks manufactured by Schoberer Rad Messtechnik (SRM). The subjects pedalled a rope-braked ergometer at 60 rev min^-1, against a resistance of 3 kg, for 5 minutes. Three different measurements of the mean power were recorded and these were compared with the value given by Monark. The SRM cranks provided two sets of results using different software packages supplied with the cranks. SRM standard software is used for taking measurements during training and cycle races over long time periods. An additional piece of software is provided by SRM called Ptnew, which gives readings of torque and pedal cadence over periods up to 30 seconds. Using the values supplied by Monark each subject generated 180 W of power. The mean power for the eight subjects, measured using the SRM cranks, was 170.36 W (SD 4.11) using the alternative SRM software (Ptnew) over a 30 second period and 173.68 W (SD 2.21) using the standard SRM software. From the direct measurement of the brake forces and flywheel velocity the mean power across the eight subjects was 148.90 W (SD 5.89). The SRM cranks measure the input power, whereas the direct measurement system measures the power output excluding mechanical losses. These values give a figure for the mechanical efficiency for the roped-braked ergometer of 88%. It was found that Monark overestimates the power generated by the subjects when compared with both the SRM systems and the direct measurement instrumentation.     

Romanticism and Pessimism: Community Education and Women

This article considers a variety of recent research projects attempting to explain the differences in examination outcomes between boys and girls, and describes some of the policies that have been introduced to overcome the 'problem'. When viewed in the light of recent findings that change the accepted picture of what, and where, these gender gaps in attainment actually are, the article discovers that there are, as yet, no totally convincing explanations of the phenomenon, and therefore little hope of an effective strategy to deal with it. This conclusion has important implications for the conduct of research, and for the links between research and evidence-based policy, and therefore for school leaders and managers.     

The effect of music therapy services on classroom behaviours of newly arrived refugee students in Australia—a pilot study

This pilot study examined the effects of a short‐term music therapy program on the classroom behaviours of newly arrived refugee students who were attending an intensive ‘English as a Second Language’ secondary school. A cross‐over design with two five‐week intervention periods was employed with group music therapy sessions conducted one or two times per week. Data from the Behaviour Assessment Scale for Children were used to evaluate a range of positive and negative school behaviours. A significant decrease in externalising behaviours was found with particular reference to hyperactivity and aggression. No significant differences were found in other behaviours. Explanations and implications of these findings are discussed.